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Escherichia coli K-12 substr. MG1655 Pathway: EvgSA Two-Component Signal Transduction System
Inferred from experimentInferred by computational analysis

Pathway diagram: EvgSA Two-Component Signal Transduction System

Diagram Key:

Locations of Mapped Genes:

Schematic showing all replicons, marked with selected genes

Genetic Regulation Schematic

Genetic regulation schematic for EvgSA Two-Component Signal Transduction System

Superclasses: Signal transduction pathways

The EvgSA two component signal transduction system (TCS) comprises EvgS - the membrane associated sensor kinase - and EvgA - the cytoplasmic response regulator. The EvgSA TCS is believed to initiate a complex transcriptional cascade that functions for acid and drug resistance in E.coli [Itou09]. Activation of the EvgSA system occurs under mild acidic conditions (pH 5-6) and in the presence of high concentrations of alkali metals [Eguchi14].

Activation of the EvgSA signal transduction system (through use of a constitutively active EvgS mutant) directly upregulates at least six genes/operons including the transcriptional regulator ydeO which then upregulates gadE - the master regulator of acid resistance genes [Itou09].

EvgS is a member of the 'unorthodox' sensor kinase class where a multistep phosphorelay occurs between alternating histidine and aspartate residues before the EvgA response regulator is activated [Appleby96]

Interaction between the EvgSA and PhoQP two-component systems has been observed in E.coli and is mediated by the small protein B1500 [Eguchi04, Eguchi07, Eguchi08].

Created 19-Jul-2010 by Mackie A, Macquarie University


Appleby96: Appleby JL, Parkinson JS, Bourret RB (1996). "Signal transduction via the multi-step phosphorelay: not necessarily a road less traveled." Cell 86(6);845-8. PMID: 8808618

Eguchi04: Eguchi Y, Okada T, Minagawa S, Oshima T, Mori H, Yamamoto K, Ishihama A, Utsumi R (2004). "Signal transduction cascade between EvgA/EvgS and PhoP/PhoQ two-component systems of Escherichia coli." J Bacteriol 186(10);3006-14. PMID: 15126461

Eguchi07: Eguchi Y, Itou J, Yamane M, Demizu R, Yamato F, Okada A, Mori H, Kato A, Utsumi R (2007). "B1500, a small membrane protein, connects the two-component systems EvgS/EvgA and PhoQ/PhoP in Escherichia coli." Proc Natl Acad Sci U S A 104(47);18712-7. PMID: 17998538

Eguchi08: Eguchi Y, Utsumi R (2008). "Introduction to bacterial signal transduction networks." Adv Exp Med Biol 631;1-6. PMID: 18792678

Eguchi14: Eguchi Y, Utsumi R (2014). "Alkali metals in addition to acidic pH activate the EvgS histidine kinase sensor in Escherichia coli." J Bacteriol. PMID: 24957621

Itou09: Itou J, Eguchi Y, Utsumi R (2009). "Molecular mechanism of transcriptional cascade initiated by the EvgS/EvgA system in Escherichia coli K-12." Biosci Biotechnol Biochem 73(4);870-8. PMID: 19352034

Utsumi92: Utsumi R, Katayama S, Ikeda M, Igaki S, Nakagawa H, Miwa A, Taniguchi M, Noda M (1992). "Cloning and sequence analysis of the evgAS genes involved in signal transduction of Escherichia coli K-12." Nucleic Acids Symp Ser (27);149-50. PMID: 1289796

Yamamoto05: Yamamoto K, Hirao K, Oshima T, Aiba H, Utsumi R, Ishihama A (2005). "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." J Biol Chem 280(2);1448-56. PMID: 15522865

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Bantscheff00: Bantscheff M, Perraud AL, Bock A, Rippe K, Weiss V, Glocker M, Gross R (2000). "Structure-function relationships in the Bvg and Evg two-component phosphorelay systems." Int J Med Microbiol 290(4-5);317-23. PMID: 11111905

Bock02: Bock A, Gross R (2002). "The unorthodox histidine kinases BvgS and EvgS are responsive to the oxidation status of a quinone electron carrier." Eur J Biochem 269(14);3479-84. PMID: 12135487

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Eguchi03: Eguchi Y, Oshima T, Mori H, Aono R, Yamamoto K, Ishihama A, Utsumi R (2003). "Transcriptional regulation of drug efflux genes by EvgAS, a two-component system in Escherichia coli." Microbiology 149(Pt 10);2819-28. PMID: 14523115

Foster04: Foster JW (2004). "Escherichia coli acid resistance: tales of an amateur acidophile." Nat Rev Microbiol 2(11);898-907. PMID: 15494746

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Hirakawa03: Hirakawa H, Nishino K, Hirata T, Yamaguchi A (2003). "Comprehensive studies of drug resistance mediated by overexpression of response regulators of two-component signal transduction systems in Escherichia coli." J Bacteriol 185(6);1851-6. PMID: 12618449

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Johnson14: Johnson MD, Bell J, Clarke K, Chandler R, Pathak P, Xia Y, Marshall RL, Weinstock GM, Loman NJ, Winn PJ, Lund PA (2014). "Characterization of mutations in the PAS domain of the EvgS sensor kinase selected by laboratory evolution for acid resistance in Escherichia coli." Mol Microbiol 93(5);911-27. PMID: 24995530

Kato00: Kato A, Ohnishi H, Yamamoto K, Furuta E, Tanabe H, Utsumi R (2000). "Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12." Biosci Biotechnol Biochem 64(6);1203-9. PMID: 10923791

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Ma03: Ma Z, Gong S, Richard H, Tucker DL, Conway T, Foster JW (2003). "GadE (YhiE) activates glutamate decarboxylase-dependent acid resistance in Escherichia coli K-12." Mol Microbiol 49(5);1309-20. PMID: 12940989

Ma04: Ma Z, Masuda N, Foster JW (2004). "Characterization of EvgAS-YdeO-GadE branched regulatory circuit governing glutamate-dependent acid resistance in Escherichia coli." J Bacteriol 186(21);7378-89. PMID: 15489450

Makar75: Makar AB, McMartin KE, Palese M, Tephly TR (1975). "Formate assay in body fluids: application in methanol poisoning." Biochem Med 1975;13(2);117-26. PMID: 1

Martin01: Martin RG, Rosner JL (2001). "The AraC transcriptional activators." Curr Opin Microbiol 4(2);132-7. PMID: 11282467

Masuda02: Masuda N, Church GM (2002). "Escherichia coli gene expression responsive to levels of the response regulator EvgA." J Bacteriol 184(22);6225-34. PMID: 12399493

Masuda03: Masuda N, Church GM (2003). "Regulatory network of acid resistance genes in Escherichia coli." Mol Microbiol 48(3);699-712. PMID: 12694615

Nishino01: Nishino K, Yamaguchi A (2001). "Overexpression of the response regulator evgA of the two-component signal transduction system modulates multidrug resistance conferred by multidrug resistance transporters." J Bacteriol 183(4);1455-8. PMID: 11157960

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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