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Escherichia coli K-12 substr. MG1655 Pathway: ArcAB Two-Component Signal Transduction System, quinone dependent
Inferred from experiment

Pathway diagram: ArcAB Two-Component Signal Transduction System, quinone dependent

Diagram Key:

Locations of Mapped Genes:

Schematic showing all replicons, marked with selected genes

Genetic Regulation Schematic

Genetic regulation schematic for ArcAB Two-Component Signal Transduction System, quinone dependent

Superclasses: Signal transduction pathways

The ArcAB two-component system mediates E. coli's response to changing respiratory conditions of growth and is a global regulator of gene expression under microaerobic and anaerobic conditions. The system comprises ArcB - a membrane associated sensor kinase and phosphatase, and ArcA - a cytosolic transcription factor. Under anaerobiosis ArcB autophosphorylates and transphosphorylates ArcA via a four step His-Asp-His-Asp phosphorelay. Activated ArcA regulates the expression of numerous operons involved in respiratory and fermentative metabolism. During aerobiosis oxidised forms of quinone electron carriers signal the inhibition of ArcB autophosphorylation and ArcB acts as a specific ArcA-P phosphatase.

Reviews: [Malpica06, Gunsalus94, Alvarez10]

Created 15-Aug-2010 by Mackie A, Macquarie University


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Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Alexeeva03: Alexeeva S, Hellingwerf KJ, Teixeira de Mattos MJ (2003). "Requirement of ArcA for redox regulation in Escherichia coli under microaerobic but not anaerobic or aerobic conditions." J Bacteriol 185(1);204-9. PMID: 12486057

Alvarez13: Alvarez AF, Rodriguez C, Georgellis D (2013). "Ubiquinone and menaquinone electron carriers represent the yin and yang in the redox regulation of the ArcB sensor kinase." J Bacteriol 195(13);3054-61. PMID: 23645604

Bekker10: Bekker M, Alexeeva S, Laan W, Sawers G, Teixeira de Mattos J, Hellingwerf K (2010). "The ArcBA two-component system of Escherichia coli is regulated by the redox state of both the ubiquinone and the menaquinone pool." J Bacteriol 192(3);746-54. PMID: 19933363

Brondsted94: Brondsted L, Atlung T (1994). "Anaerobic regulation of the hydrogenase 1 (hya) operon of Escherichia coli." J Bacteriol 176(17);5423-8. PMID: 8071220

Cho06: Cho BK, Knight EM, Palsson BO (2006). "Transcriptional regulation of the fad regulon genes of Escherichia coli by ArcA." Microbiology 152(Pt 8);2207-19. PMID: 16849788

Compan94: Compan I, Touati D (1994). "Anaerobic activation of arcA transcription in Escherichia coli: roles of Fnr and ArcA." Mol Microbiol 1994;11(5);955-64. PMID: 8022271

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Drury85: Drury LS, Buxton RS (1985). "DNA sequence analysis of the dye gene of Escherichia coli reveals amino acid homology between the dye and OmpR proteins." J Biol Chem 1985;260(7);4236-42. PMID: 2984198

Favorov05: Favorov AV, Gelfand MS, Gerasimova AV, Ravcheev DA, Mironov AA, Makeev VJ (2005). "A Gibbs sampler for identification of symmetrically structured, spaced DNA motifs with improved estimation of the signal length." Bioinformatics 21(10);2240-5. PMID: 15728117

Gao08: Gao R, Tao Y, Stock AM (2008). "System-level mapping of Escherichia coli response regulator dimerization with FRET hybrids." Mol Microbiol 69(6);1358-72. PMID: 18631241

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Georgellis01: Georgellis D, Kwon O, Lin EC (2001). "Quinones as the redox signal for the arc two-component system of bacteria." Science 292(5525);2314-6. PMID: 11423658

Georgellis97: Georgellis D, Lynch AS, Lin EC (1997). "In vitro phosphorylation study of the arc two-component signal transduction system of Escherichia coli." J Bacteriol 1997;179(17);5429-35. PMID: 9286997

Georgellis98: Georgellis D, Kwon O, De Wulf P, Lin EC (1998). "Signal decay through a reverse phosphorelay in the Arc two-component signal transduction system." J Biol Chem 273(49);32864-9. PMID: 9830034

Georgellis99: Georgellis D, Kwon O, Lin EC (1999). "Amplification of signaling activity of the arc two-component system of Escherichia coli by anaerobic metabolites. An in vitro study with different protein modules." J Biol Chem 274(50);35950-4. PMID: 10585483

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Govantes00: Govantes F, Orjalo AV, Gunsalus RP (2000). "Interplay between three global regulatory proteins mediates oxygen regulation of the Escherichia coli cytochrome d oxidase (cydAB) operon." Mol Microbiol 38(5);1061-73. PMID: 11123679

Hoch95: Hoch, JA, Silhavy, TJ "Two-Component Signal Transduction." ASM Press, Washington, D.C. 1995.

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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