Escherichia coli K-12 substr. MG1655 RNA: McaS small regulatory RNA

Gene: mcaS Accession Numbers: G0-8899 (EcoCyc), b4426, ECK1336

Synonyms: IS061, isrA

Superclasses: a regulatory RNA

Regulation Summary Diagram: ?

Regulation summary diagram for mcaS

McaS is a Hfq-binding small RNA that regulates the expression of curli by inhibiting translation and enhancing degradation of csgD mRNA. Conversely, it positively regulates flagellar motility and biofilm formation [Jorgensen12, Thomason12, De12]. McaS not only acts as a direct regulator of translation by base-pairing with the 5' UTRs of mRNAs, but also indirectly by binding and sequestering CsrA, a global post-transcriptional regulator. In addition to pgaABCD, other regulatory targets of CsrA, including dgcZ, ycdT and glgC, are thereby indicrectly regulated by McaS [Jorgensen13].

McaS has at least two GGA sequence elements that function as CsrA-binding sequences; McaS binds CsrA with an apparent Kd of 0.71 nM [Jorgensen13].

The half life of McaS is strongly reduced in the absence of Hfq. mcaS expression in rich medium is highest during the transition from log to stationary phase; in minimal medium with glycerol, expression is highest during logarithmic growth [Jorgensen12, Thomason12]. Overexpression of mcaS inhibits production of curli and lowers the expression of csgD [Jorgensen12]. Conversely, it positively regulates expression of flhD and pgaA, leading to increased motility and biofilm formation [Thomason12, De12, Jorgensen13].

McaS (IS061) was first identified as a potential small RNA of approximately 85-90 nt in length [Chen02].

Using a network biology approach, both GlmZ and McaS (IsrA) were predicted to be involved in the DNA damage response. A glmZ mcaS double mutant is less sensitive to DNA damaging agents and has a lower basal mutation rate than wild type [Modi11].

McaS: "multi-cellular adhesive sRNA" [Jorgensen12, Thomason12]

Reviews: [Hershberg03, Holmqvist13]

Map Position: [1,403,683 <- 1,403,775] (30.25 centisomes, 109°)
Length: 93 bp

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for mcaS

Unification Links: ASAP:ABE-0047247 , EchoBASE:EB4510 , EcoGene:EG31143 , EcoliWiki:b4426 , PortEco:isrA , RegulonDB:G0-8899

GO Terms:

Biological Process: GO:0045947 - negative regulation of translational initiation Inferred from experiment [Jorgensen12]
GO:0045948 - positive regulation of translational initiation Inferred from experiment [Thomason12]
GO:0051100 - negative regulation of binding Inferred from experiment [Jorgensen13]
GO:0070928 - regulation of mRNA stability, ncRNA-mediated Inferred from experiment [Jorgensen12]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Jorgensen13, Jorgensen12]
GO:0048027 - mRNA 5'-UTR binding Inferred from experiment [Jorgensen12]

MultiFun Terms: information transfer RNA related antisense RNA
regulation type of regulation posttranscriptional antisense RNA
regulation type of regulation posttranscriptional inhibition / activation of enzymes
regulation type of regulation posttranscriptional translation attenuation and efficiency

Regulated Transcription Units (2 total): ?


Transcription-unit diagram

Transcription-unit diagram

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Ingrid Keseler on Wed Jan 25, 2012:
Gene coordinates updated based on information in [Jorgensen12 ].
Suzanne Paley on Thu Oct 21, 2004:
Position updated based on U00096.2 release of genome

Last-Curated ? 02-Aug-2013 by Keseler I , SRI International


Chen02: Chen S, Lesnik EA, Hall TA, Sampath R, Griffey RH, Ecker DJ, Blyn LB (2002). "A bioinformatics based approach to discover small RNA genes in the Escherichia coli genome." Biosystems 65(2-3);157-77. PMID: 12069726

De12: De Lay N, Gottesman S (2012). "A complex network of small non-coding RNAs regulate motility in Escherichia coli." Mol Microbiol 86(3);524-38. PMID: 22925049

Hershberg03: Hershberg R, Altuvia S, Margalit H (2003). "A survey of small RNA-encoding genes in Escherichia coli." Nucleic Acids Res 31(7);1813-20. PMID: 12654996

Holmqvist13: Holmqvist E, Vogel J (2013). "A small RNA serving both the Hfq and CsrA regulons." Genes Dev 27(10);1073-8. PMID: 23699406

Jorgensen12: Jorgensen MG, Nielsen JS, Boysen A, Franch T, Moller-Jensen J, Valentin-Hansen P (2012). "Small regulatory RNAs control the multi-cellular adhesive lifestyle of Escherichia coli." Mol Microbiol 84(1);36-50. PMID: 22250746

Jorgensen13: Jorgensen MG, Thomason MK, Havelund J, Valentin-Hansen P, Storz G (2013). "Dual function of the McaS small RNA in controlling biofilm formation." Genes Dev 27(10);1132-45. PMID: 23666921

Modi11: Modi SR, Camacho DM, Kohanski MA, Walker GC, Collins JJ (2011). "Functional characterization of bacterial sRNAs using a network biology approach." Proc Natl Acad Sci U S A 108(37);15522-7. PMID: 21876160

Thomason12: Thomason MK, Fontaine F, De Lay N, Storz G (2012). "A small RNA that regulates motility and biofilm formation in response to changes in nutrient availability in Escherichia coli." Mol Microbiol 84(1);17-35. PMID: 22289118

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Mon Mar 30, 2015, biocyc14.