|Gene:||sibB||Accession Numbers: G0-8885 (EcoCyc), b4437, ECK2069|
Synonyms: QUAD1b, tpe60, ryeD
Superclasses: a regulatory RNA
The SibB small RNA was first detected during a comparative genomics screen for sequence conservation [Wassarman01]. The yegL-yegM intergenic region was initially shown to contain two copies of a repeat sequence element (initially termed QUAD1 and later renamed SIB) that occurs five times in the E. coli K-12 genome [Rudd99, Fozo08]. Many sequence regions conserved between the SIB elements are predicted to form stem-loop structures [Fozo08].
Two RNA products arising from sibB have been detected [Wassarman01, Fozo08]; the shorter RNA product may be derived by processing of the longer transcript [Fozo08]. A small open reading frame encoding the predicted toxic peptide IbsB is located on the opposite strand [Fozo08].
Deletion of sibB has no detectable effect on cell growth. However, in a sibB deletion strain, expression of SibB from a plasmid must be highly induced to yield viable cells; this appears to be due to overexpression of the toxic peptide IbsB [Fozo08].
Ryx: "RNA of unknown function at X position (10 minute) in the genome" [Wassarman01]
SibA: "short, intergenic, abundant sequence" [Fozo08]
|Map Position: [2,151,670 -> 2,151,805] (46.38 centisomes, 167°)||Length: 136 bp|
|Biological Process:||GO:0035194 - posttranscriptional gene silencing by RNA [Fozo08]|
|MultiFun Terms:||information transfer → RNA related → antisense RNA|
Ingrid Keseler on Wed Aug 27, 2008:
Gene start and end positions corrected based on [Fozo08 ].
Ingrid Keseler on Thu May 31, 2007:
Gene position corrected based on information provided by Gisela Storz.
Suzanne Paley on Thu Oct 21, 2004:
Gene boundaries added from U00096.2 release of genome
Fozo08: Fozo EM, Kawano M, Fontaine F, Kaya Y, Mendieta KS, Jones KL, Ocampo A, Rudd KE, Storz G (2008). "Repression of small toxic protein synthesis by the Sib and OhsC small RNAs." Mol Microbiol 70(5):1076-93. PMID: 18710431
Gottesman01: Gottesman S, Storz G, Rosenow C, Majdalani N, Repoila F, Wassarman KM (2001). "Small RNA regulators of translation: mechanisms of action and approaches for identifying new small RNAs." Cold Spring Harb Symp Quant Biol 66;353-62. PMID: 12762038
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