|Superclasses:||a modified protein|
AsbD is an aryl-carrier protein that activates protocatechuate prior to its incorporation into petrobactin. The AsbD protein itself needs to be activated by the transfer of a 4'-phosphopantetheine group from coenzyme A to serine 40 of the apo form, resulting in the active holo form, in a manner similar to other acyl- and aryl-carrier proteins.
Once activated, the protocatechuate moiety can be transferred by AsbE from a 3,4-DHB-AsbD protein to different receipient molecules, including N-citryl-spermidine, N8,N'8-citryl-bis(spermidine) and N1-(3,4-dihydroxybenzoyl)-N8,N'8-citryl-bis(spermidine) [OvesCostales08].
Reactions known to consume the compound:
N8,N'8-citryl-bis(spermidine) + a 3,4-DHB-AsbD protein → N1-(3,4-dihydroxybenzoyl)-N8,N'8-citryl-bis(spermidine) + a holo-AsbD protein + H+
N1-(3,4-dihydroxybenzoyl)-N8,N'8-citryl-bis(spermidine) + a 3,4-DHB-AsbD protein → petrobactin + H2O
a 3,4-DHB-AsbD protein + N-citryl-spermidine → N1-(3,4-dihydroxybenzoyl)-N8-citryl-spermidine + a holo-AsbD protein
Reactions known to produce the compound:
OvesCostales08: Oves-Costales D, Kadi N, Fogg MJ, Song L, Wilson KS, Challis GL (2008). "Petrobactin biosynthesis: AsbB catalyzes condensation of spermidine with N8-citryl-spermidine and its N1-(3,4-dihydroxybenzoyl) derivative." Chem Commun (Camb) (34);4034-6. PMID: 18758617
Pfleger07: Pfleger BF, Lee JY, Somu RV, Aldrich CC, Hanna PC, Sherman DH (2007). "Characterization and analysis of early enzymes for petrobactin biosynthesis in Bacillus anthracis." Biochemistry 46(13);4147-57. PMID: 17346033
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