MetaCyc Protein Class: an apo-[aryl-carrier protein]

Superclasses: a polypeptideall acyl-carrier proteinsan apo-[acyl-carrier protein]
a polypeptideall acyl-carrier proteinsan aryl-carrier protein
a proteinall acyl-carrier proteinsan apo-[acyl-carrier protein]
a proteinall acyl-carrier proteinsan aryl-carrier protein

Aryl carrier proteins (ArCP) are a common component of non-ribosomal peptide synthetase (NRPS) complexes. The protein is expressed in the inactive apo form and the essential prosthetic group 4'-phosphopantetheine must be post-translationally attached to a conserved serine residue by the action of a phosphopantetheinyl transferase.

an <i>apo</i>-[aryl-carrier protein] compound structure

Child Classes: a 3-hydroxy-4-methyl-anthranilyl-L-threonyl-D-valyl-[AcmD acyl-carrier protein] (0), an apo-AsbD acyl-carrier protein (1), an apo-[AcmD acyl-carrier protein] (1), an apo-[EntB isochorismatase/aryl-carrier protein] (1), an apo-[SibE aryl-carrier protein] (1), an NRPS containing an [aryl-carrier-protein] domain (0)

Alternative forms of an apo-[aryl-carrier protein]: a holo-[aryl-carrier protein]

SMILES: C(O)C(N[an aryl-carrier protein])C(=O)[an aryl-carrier protein]

Reactions known to consume the compound:

acyl carrier protein activation , acyl carrier protein metabolism :
an acyl-carrier protein + coenzyme A → adenosine 3',5'-bisphosphate + a holo-[acyl-carrier protein] + H+

Reactions known to produce the compound:

acyl carrier protein metabolism :
a holo-[acyl-carrier protein] + H2O → 4'-phosphopantetheine + an acyl-carrier protein + H+

Enzymatic reactions inhibited by an apo-[aryl-carrier protein], sorted by the type of inhibition, are:

Inhibitor (Mechanism unknown) of: acyl-CoA:1-acyl-sn-glycerol-3-phosphate 2-O-acyltransferase [Rock81, Comment 1] Inhibitor (Other types) of: acetyl-CoA carboxylase [Davis01]

Gene-Reaction Schematic

Gene-Reaction Schematic

Instance reactions of [an acyl-carrier protein + coenzyme A → adenosine 3',5'-bisphosphate + a holo-[acyl-carrier protein] + H+] (
i1: AsbD acyl-carrier protein + coenzyme A → adenosine 3',5'-bisphosphate + a holo-[AsbD acyl-carrier protein] + H+ (no EC#)

i2: [EntB aryl-carrier protein] + coenzyme A → a holo-[EntB isochorismatase/aryl-carrier protein] + adenosine 3',5'-bisphosphate + H+ (


Byers07: Byers DM, Gong H (2007). "Acyl carrier protein: structure-function relationships in a conserved multifunctional protein family." Biochem Cell Biol 85(6);649-62. PMID: 18059524

Davis01: Davis MS, Cronan, Jr. JE (2001). "Inhibition of Escherichia coli acetyl coenzyme A carboxylase by acyl-acyl carrier protein." J Bacteriol. 183(4):1499-503. PMID: 11157970

Gehring97: Gehring AM, Bradley KA, Walsh CT (1997). "Enterobactin biosynthesis in Escherichia coli: isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoate." Biochemistry 1997;36(28);8495-503. PMID: 9214294

Gehring98: Gehring AM, Mori I, Walsh CT (1998). "Reconstitution and characterization of the Escherichia coli enterobactin synthetase from EntB, EntE, and EntF." Biochemistry 1998;37(8);2648-59. PMID: 9485415

Giessen11: Giessen TW, Kraas FI, Marahiel MA (2011). "A four-enzyme pathway for 3,5-dihydroxy-4-methylanthranilic acid formation and incorporation into the antitumor antibiotic sibiromycin." Biochemistry 50(25);5680-92. PMID: 21612226

OvesCostales08: Oves-Costales D, Kadi N, Fogg MJ, Song L, Wilson KS, Challis GL (2008). "Petrobactin biosynthesis: AsbB catalyzes condensation of spermidine with N8-citryl-spermidine and its N1-(3,4-dihydroxybenzoyl) derivative." Chem Commun (Camb) (34);4034-6. PMID: 18758617

Pfennig99: Pfennig F, Schauwecker F, Keller U (1999). "Molecular characterization of the genes of actinomycin synthetase I and of a 4-methyl-3-hydroxyanthranilic acid carrier protein involved in the assembly of the acylpeptide chain of actinomycin in Streptomyces." J Biol Chem 274(18);12508-16. PMID: 10212227

Pfleger07: Pfleger BF, Lee JY, Somu RV, Aldrich CC, Hanna PC, Sherman DH (2007). "Characterization and analysis of early enzymes for petrobactin biosynthesis in Bacillus anthracis." Biochemistry 46(13);4147-57. PMID: 17346033

Rock81: Rock CO, Goelz SE, Cronan JE (1981). "Phospholipid synthesis in Escherichia coli. Characteristics of fatty acid transfer from acyl-acyl carrier protein to sn-glycerol 3-phosphate." J Biol Chem 1981;256(2);736-42. PMID: 7005223

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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