MetaCyc Protein Class: an apo-AsbD acyl-carrier protein

Abbrev Name: AsbD acyl-carrier protein

Superclasses: a polypeptideall acyl-carrier proteinsan apo-[acyl-carrier protein]an apo-[aryl-carrier protein]
a polypeptideall acyl-carrier proteinsan aryl-carrier proteinan apo-[aryl-carrier protein]
a proteinall acyl-carrier proteinsan apo-[acyl-carrier protein]an apo-[aryl-carrier protein]
a proteinall acyl-carrier proteinsan aryl-carrier proteinan apo-[aryl-carrier protein]

AsbD is an aryl-carrier protein that activates protocatechuate prior to its incorporation into petrobactin. The AsbD protein itself needs to be activated by the transfer of a 4'-phosphopantetheine group from coenzyme A to serine 40 of the apo form, resulting in the active holo form, in a manner similar to other acyl- and aryl-carrier proteins.

The AsbC protein catalyzes the adenylation of protocatechuate, followed by the transfer of the adenylated intermediate to the holo-asbD protein, resulting in a 3,4-dihydroxybenzoyl-[AsbD acyl-carrier protein] [Pfleger07].

Once activated, the protocatechuate moiety can be transferred by AsbE from a 3,4-dihydroxybenzoyl-[AsbD acyl-carrier protein] to different receipient molecules, including N-citryl-spermidine, N8,N'8-citryl-bis(spermidine) and N1-(3,4-dihydroxybenzoyl)-N8,N'8-citryl-bis(spermidine) [OvesCostales08].

an apo-AsbD acyl-carrier protein compound structure

AsbD aryl-carrier protein (Bacillus anthracis)

Alternative forms of an apo-AsbD acyl-carrier protein: a holo-[AsbD acyl-carrier protein] (summary available)

SMILES: C(O)C(N[an apo-AsbD acyl-carrier protein])C(=O)[an apo-AsbD acyl-carrier protein]

Reactions known to consume the compound:

petrobactin biosynthesis :
AsbD acyl-carrier protein + coenzyme A → adenosine 3',5'-bisphosphate + a holo-[AsbD acyl-carrier protein] + H+

acyl carrier protein activation , acyl carrier protein metabolism :
an acyl-carrier protein + coenzyme A → adenosine 3',5'-bisphosphate + a holo-[acyl-carrier protein] + H+

Reactions known to produce the compound:

acyl carrier protein metabolism :
a holo-[acyl-carrier protein] + H2O → 4'-phosphopantetheine + an acyl-carrier protein + H+

Enzymes inhibited by an apo-AsbD acyl-carrier protein, sorted by the type of inhibition, are:

Inhibitor (Mechanism unknown) of: acyl-CoA:1-acyl-sn-glycerol-3-phosphate 2-O-acyltransferase [Rock81, Comment 1] Inhibitor (Other types) of: acetyl-CoA carboxylase [Davis01]

Gene-Reaction Schematic

Gene-Reaction Schematic

Created 30-Jun-2009 by Caspi R, SRI International


Byers07: Byers DM, Gong H (2007). "Acyl carrier protein: structure-function relationships in a conserved multifunctional protein family." Biochem Cell Biol 85(6);649-62. PMID: 18059524

Davis01: Davis MS, Cronan, Jr. JE (2001). "Inhibition of Escherichia coli acetyl coenzyme A carboxylase by acyl-acyl carrier protein." J Bacteriol. 183(4):1499-503. PMID: 11157970

OvesCostales08: Oves-Costales D, Kadi N, Fogg MJ, Song L, Wilson KS, Challis GL (2008). "Petrobactin biosynthesis: AsbB catalyzes condensation of spermidine with N8-citryl-spermidine and its N1-(3,4-dihydroxybenzoyl) derivative." Chem Commun (Camb) (34);4034-6. PMID: 18758617

Pfleger07: Pfleger BF, Lee JY, Somu RV, Aldrich CC, Hanna PC, Sherman DH (2007). "Characterization and analysis of early enzymes for petrobactin biosynthesis in Bacillus anthracis." Biochemistry 46(13);4147-57. PMID: 17346033

Rock81: Rock CO, Goelz SE, Cronan JE (1981). "Phospholipid synthesis in Escherichia coli. Characteristics of fatty acid transfer from acyl-acyl carrier protein to sn-glycerol 3-phosphate." J Biol Chem 1981;256(2);736-42. PMID: 7005223

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.5 on Mon Nov 30, 2015, BIOCYC14A.