Abbrev Name: AsbD acyl-carrier protein
|Superclasses:||a polypeptide → all acyl-carrier proteins → an apo-[acyl-carrier protein] → an apo-[aryl-carrier protein]|
|a polypeptide → all acyl-carrier proteins → an aryl-carrier protein → an apo-[aryl-carrier protein]|
|a protein → all acyl-carrier proteins → an apo-[acyl-carrier protein] → an apo-[aryl-carrier protein]|
|a protein → all acyl-carrier proteins → an aryl-carrier protein → an apo-[aryl-carrier protein]|
AsbD is an aryl-carrier protein that activates protocatechuate prior to its incorporation into petrobactin. The AsbD protein itself needs to be activated by the transfer of a 4'-phosphopantetheine group from coenzyme A to serine 40 of the apo form, resulting in the active holo form, in a manner similar to other acyl- and aryl-carrier proteins.
The AsbC protein catalyzes the adenylation of protocatechuate, followed by the transfer of the adenylated intermediate to the holo-asbD protein, resulting in a 3,4-dihydroxybenzoyl-[AsbD acyl-carrier protein] [Pfleger07].
Once activated, the protocatechuate moiety can be transferred by AsbE from a 3,4-dihydroxybenzoyl-[AsbD acyl-carrier protein] to different receipient molecules, including N-citryl-spermidine, N8,N'8-citryl-bis(spermidine) and N1-(3,4-dihydroxybenzoyl)-N8,N'8-citryl-bis(spermidine) [OvesCostales08].
AsbD aryl-carrier protein (Bacillus anthracis)
Alternative forms of an apo-AsbD acyl-carrier protein: a holo-[AsbD acyl-carrier protein] (summary available)
SMILES: C(O)C(N[an apo-AsbD acyl-carrier protein])C(=O)[an apo-AsbD acyl-carrier protein]
Reactions known to consume the compound:
Reactions known to produce the compound:
Enzymes inhibited by an apo-AsbD acyl-carrier protein, sorted by the type of inhibition, are:
Inhibitor (Mechanism unknown) of: acyl-CoA:1-acyl-sn-glycerol-3-phosphate 2-O-acyltransferase [Rock81, Comment 1]
OvesCostales08: Oves-Costales D, Kadi N, Fogg MJ, Song L, Wilson KS, Challis GL (2008). "Petrobactin biosynthesis: AsbB catalyzes condensation of spermidine with N8-citryl-spermidine and its N1-(3,4-dihydroxybenzoyl) derivative." Chem Commun (Camb) (34);4034-6. PMID: 18758617
Pfleger07: Pfleger BF, Lee JY, Somu RV, Aldrich CC, Hanna PC, Sherman DH (2007). "Characterization and analysis of early enzymes for petrobactin biosynthesis in Bacillus anthracis." Biochemistry 46(13);4147-57. PMID: 17346033
Rock81: Rock CO, Goelz SE, Cronan JE (1981). "Phospholipid synthesis in Escherichia coli. Characteristics of fatty acid transfer from acyl-acyl carrier protein to sn-glycerol 3-phosphate." J Biol Chem 1981;256(2);736-42. PMID: 7005223
©2014 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493