MetaCyc Gene-Ontology-Terms Class: GO:0016787 - hydrolase activity

Definition: Catalysis of the hydrolysis of various bonds, e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc. Hydrolase is the systematic name for any enzyme of EC class 3.

Parent Classes:
GO:0003824 - catalytic activity

Child Classes:
GO:0008233 - peptidase activity (78),
GO:0016788 - hydrolase activity, acting on ester bonds (191),
GO:0016798 - hydrolase activity, acting on glycosyl bonds (55),
GO:0016801 - hydrolase activity, acting on ether bonds (5),
GO:0016810 - hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds (82),
GO:0016817 - hydrolase activity, acting on acid anhydrides (198),
GO:0016822 - hydrolase activity, acting on acid carbon-carbon bonds (4),
GO:0017171 - serine hydrolase activity (30),
GO:0019213 - deacetylase activity (7),
GO:0051747 - cytosine C-5 DNA demethylase activity (1)

Term Members:
endochitinase (chiA),
N-acetylmuramyl-L-alanine amidase C (amiC),
30S ribosomal subunit protein S16 (rpsP),
MazF toxin of the MazF-MazE toxin-antitoxin system that exhibits ribonuclease activity (chpA),
N-acetylmuramoyl-L-alanine amidase A (amiA),
penicillin-binding protein 6 (dacC),
primosome factor N' (priA),
EnvC divisome associated factor, activator of peptidoglycan hydrolases,
N-acetylmuramoyl-L-alanine amidase B (amiB),
D-Ala-D-Ala dipeptidase (ddpX),
DD-carboxypeptidase, penicillin-binding protein 6b (dacD),
RPnTP hydrolase (phnM),
5-phospho-α-D-ribosyl 1,2-cyclic phosphate phosphodiesterase (phnP),
acyl carrier protein phosphodiesterase (acpH),
GTP cyclohydrolase I monomer (folE),
NMN aminohydrolase (pncC),
nicotinamidase / pyrazinamidase (pncA),
periplasmic trehalase (treA),
cytoplasmic trehalase (treF),
trehalose-6-phosphate hydrolase (treC),
β-D-glucuronidase (uidA),
glucosamine-6-phosphate deaminase (nagB),
N-acetylglucosamine-6-phosphate deacetylase (nagA),
glutaminase (yneH),
glutaminase (ybaS),
fructoselysine 6-phosphate deglycase monomer (frlB),
N-acetyl-anhydromuramyl-L-alanine amidase (ampD),
anhydro-N-acetylmuramoyl-L-alanine amidase,
γ-glutamyl-γ-aminobutyrate hydrolase (puuD),
guanosine 3'-diphosphate 5'-triphosphate 3'-diphosphatase [multifunctional] (spoT),
α-galactosidase monomer (melA),
α-D-glucose-1-phosphatase (yihX),
α-mannosidase (mngB),
L,D-transpeptidase ErfK (ldtA),
L,D-transpeptidase YbiS (ldtB),
L,D-transpeptidase YcfS (ldtC),
allantoinase monomer (allB),
allantoate amidohydrolase monomer (allC),
S-ureidoglycine aminohydrolase (allE),
predicted adenosylcobalamin phosphatase/α-ribazole phosphatase (cobC),
asparaginase II (ansB),
L-ascorbate 6-phosphate lactonase (ulaG),
2-hydroxy-6-ketonona-2,4-dienedioate hydrolase (mhpC),
guanine deaminase (guaD),
adenosine deaminase (add),
glyoxalase III, Hsp31 molecular chaperone (hchA),
AICAR transformylase / IMP cyclohydrolase (purH),
glyoxalase II (gloB),
glyoxalase II (gloC),
isopentenyl diphosphate isomerase (idi),
phosphatidylglycerophosphatase A (pgpA),
phosphatidylglycerophosphatase C (pgpC),
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH),
1,4-dihydroxy-2-naphthoyl-CoA thioesterase (menI),
phosphoserine phosphatase (serB),
ribonucleoside hydrolase 1 (pyrimidine-specific) (rihA),
RNase P protein component; processes tRNA, 4.5S RNA (rnpA),
fructose-1,6-bisphosphatase monomer (fbp),
fructose 1,6-bisphosphatase II (glpX),
fructose-1,6-bisphosphatase (yggF),
6-phosphogluconolactonase (pgl),
N-succinyl-L-diaminopimelate desuccinylase subunit (dapE),
trehalose-6-phosphate phosphatase (otsB),
phosphoribosyl-AMP cyclohydrolase / phosphoribosyl-ATP pyrophosphatase (hisI),
D,D-heptose 1,7-bisphosphate phosphatase (gmhB),
UDP-2,3-diacylglucosamine hydrolase (lpxH),
UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC),
thioesterase III (fadM),
S-formylglutathione hydrolase (frmB),
fused diaminohydroxyphosphoribosylaminopyrimidine deaminase / 5-amino-6-(5-phosphoribosylamino)uracil reductase (ribD),
GTP cyclohydrolase II (ribA),
succinylglutamate desuccinylase (astE),
limit dextrin α-1,6-glucohydrolase (glgX),
undecaprenyl pyrophosphate phosphatase (bacA),
undecaprenyl pyrophosphate phosphatase (ybjG),
glucose-1-phosphatase (agp),
oligoribonuclease monomer (orn),
β-D-glucoside glucohydrolase, periplasmic (bglX),
exonuclease VIII, ds DNA exonuclease, 5' --> 3' specific (recE),
α-amylase (malS),
α-amylase (amyA),
protease IV (sppA),
endonuclease IV (nfo),
formamidopyrimidine DNA glycosylase (mutM),
leader peptidase (signal peptidase I) (lepB),
aminopeptidase (ypdF),
crRNA endonuclease (casE),
pimeloyl-[acp] methyl ester esterase (bioH),
uracil-DNA glycosylase (ung),
alkaline phosphatase isozyme conversion protein (iap),
branch migration of Holliday structures; repair (ruvA),
branch migration of Holliday structures; repair helicase (ruvB),
Holliday junction nuclease; resolution of structures; repair (ruvC),
peptidase T,
leader peptidase, integral membrane protein (gspO),
5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase (yigB),
5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase (ybjI),
ribonucleoside hydrolase 2 (pyrimidine-specific) (rihB),
protein-lysine deacetylase and desuccinylase (cobB),
phosphoglycolate phosphatase (gph),
RNase III (rnc),
peptidase E, a dipeptidase where amino-terminal residue is aspartate (pepE),
peptide deformylase,
L,D-transpeptidase LdtD,
L,D-transpeptidase LdtE,
mRNA interferase, toxin of the MqsR-MqsA toxin-antitoxin system,
CP4-57 prophage; RNase LS, toxin of the RnlAB toxin-antitoxin system,
antitoxin of the GhoTS toxin-antitoxin system (ghoS),
chito-oligosaccharide mono-deacetylase (chbG),
DNA repair; excision nuclease subunit B (uvrB),
exonuclease I, 3' --> 5' specific; deoxyribophosphodiesterase (sbcB),
ssDNA/RNA exonuclease, 3' → 5' specific (tatD),
inorganic triphosphatase (ygiF),
dipeptidyl carboxypeptidase II (dcp),
adenosine-3'(2'),5'-bisphosphate nucleotidase (cysQ),
ADP-sugar pyrophosphatase (nudF),
ADP-sugar pyrophosphorylase (nudE),
β-phosphoglucomutase (ycjU),
ribonuclease G (RNAse G) monomer (rng),
RNA/ssDNA exonuclease 5' → 3' specific (yciV),
DLP12 prophage; lysozyme (rrrD),
cAMP phosphodiesterase (cpdA),
GDP-mannose hydrolase (nudK),
monoacetylchitobiose-6-phosphate hydrolase (chbF),
poly-β-1,6-N-acetyl-D-glucosamine N-deacetylase (pgaB),
RNase I, cleaves phosphodiester bond between any two nucleotides,
proofreading thioesterase in enterobactin biosynthesis (entH),
exopolyphosphatase (ppx),
phenylhydantoinase (hyuA),
Deg S monomer (degS),
serine endoprotease, periplasmic monomer (degQ),
DNA-specific endonuclease I (endA),
3-methyl-adenine DNA glycosylase II, inducible (alkA),
outer membrane protease VII (outer membrane protein 3b) (ompT),
broad-specificity exoaminopeptidase (ypdE),
D-Tyr-tRNATyr deacylase (dtd),
toxin of the YoeB-YefM toxin-antitoxin pair,
RNA 2',3'-cyclic phosphodiesterase (thpR),
aminopeptidase A/I (pepA),
PII uridylyltransferase / uridylyl removing enzyme (glnD),
protein-tyrosine phosphatase (wzb),
phosphotyrosine-protein phosphatase (etp),
γ-glutamyltranspeptidase (ggt),
aminopeptidase B (pepB),
phosphoprotein phosphatase 1 (pphA),
phosphoprotein phosphatase 2 (pphB),
endonuclease V (nfi),
prolipoprotein signal peptidase II (lspA),
fructose-1-phosphatase (yqaB),
6-phospho-β-glucosidase B; cryptic (bglB),
endo-1,4-D-glucanase (bcsZ),
O-acetyl-ADP-ribose deacetylase, regulator of RNase III activity (ymdB),
restriction of DNA at 5-methylcytosine residues (mcrA),
hydrogenase 3 maturation protease (hycI),
peptidase D (pepD),
oligopeptidase A (prlC),
phenylacetyl-CoA thioesterase (paaI),
peptidyl-tRNA hydrolase (pth),
peptidyl-tRNA hydrolase, ribosome rescue factor (arfB),
arylsulfatase (aslA),
ribosome-dependent mRNA interferase, toxin of the YafO-YafN toxin-antitoxin system,
translation-dependent mRNA interferase, toxin of the HigB-HigA toxin-antitoxin system,
toxin of the YafQ-DinJ toxin-antitoxin system,
Qin prophage; toxin of the RelE-RelB toxin-antitoxin system and cofactor to enhance the repressor activity of RelB,
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase monomer (mtn),
5-hydroxy-CTP diphosphatase (nudG),
DNA polymerase III, ε subunit (dnaQ),
SOS mutagenesis; error-prone repair; processed to UmuD'; forms complex with UmuC,
DNA polymerase I, 5' --> 3' polymerase, 5' --> 3' and 3' --> 5' exonuclease (polA),
phosphatidylglycerophosphatase [multifunctional] (pgpB),
L,D-carboxypeptidase A (ldcA),
Xaa-Pro dipeptidase (pepQ),
diadenosine tetraphosphatase (apaH),
acyl-CoA thioesterase (yciA),
β-lactamase; penicillin resistance (ampC),
D-alanyl-D-alanine carboxypeptidase, fraction A; penicillin-binding protein 5 (dacA),
predicted NUDIX hydrolase with low 3-phosphohydroxypyruvate phosphatase activity (nudL),
GTPase, involved in modification of U34 in tRNA (mnmE),
exonuclease VII, small subunit (xseB),
exonuclease VII, large subunit (xseA),
pyridoxal phosphatase / fructose 1,6-bisphosphatase (ybhA),
phosphodiesterase, c-di-GMP-specific (pdeL),
c-di-GMP phosphodiesterase, heme-regulated (dosP),
cyclic di-GMP phosphodiesterase (pdeR),
c-di-GMP phosphodiesterase (pdeH),
cyclic di-GMP phosphodiesterase (pdeF),
tail-specific protease (prc),
pyrimidine deoxynucleoside triphosphate pyrophosphohydrolase (nudI),
acylphosphatase (yccX),
β-galactosidase monomer (lacZ),
lysophospholipase L2 (pldB),
deoxyguanosine triphosphate triphosphohydrolase (dgt),
Rac prophage; DNA endonuclease toxin of the RalRA toxin-antitoxin system, restriction alleviation and modification enhancement,
tRNA-specific adenosine deaminase monomer (tadA),
RNase D (rnd),
β-N-acetylhexosaminidase (nagZ),
Rep helicase,
ATP-dependent DNA helicase (recQ),
oligopeptidase B (ptrB),
p-aminobenzoyl-glutamate hydrolase subunit A (abgA),
p-aminobenzoyl-glutamate hydrolase subunit B (abgB),
acetyl esterase (aes),
cryptic adenine deaminase monomer (adeD),
YhaV toxic ribonuclease,
stationary phase mismatch/uracil DNA glycosylase (mug),
sugar phosphatase (ybiV),
sugar phosphatase (yfbT),
sugar phosphatase (yidA),
phosphosugar phosphatase (yigL),
inositol monophosphatase (suhB),
ATP-dependent zinc metalloprotease FtsH,
outer membrane metallopeptidase (loiP),
host restriction; endonuclease R (hsdR),
dihydroneopterin triphosphate pyrophosphohydrolase (nudB),
methionine aminopeptidase (map),
peroxyureidoacrylate / ureidoacrylate amido hydrolase (rutB),
8-oxo-dGTP diphosphatase (mutT),
6-phosphogluconate phosphatase (yieH),
maltodextrin glucosidase (malZ),
6-phospho-β-glucosidase; cryptic (ascB),
regulatory ATPase (ravA),
alkyl sulfatase (yjcS),
endonuclease III (nth),
MrcB subunit of 5-methylcytosine restriction system (mcrB),
oxepin-CoA hydrolase/3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase (paaZ),
NADH pyrophosphatase (nudC),
protease III (ptrA),
ribonucleoside hydrolase 3 (rihC),
UDP-sugar hydrolase / 5'-ribonucleotidase / 5'-deoxyribonucleotidase (ushA),
pyrimidine nucleotidase (yjjG),
UMP phosphatase (umpH),
3-methyl-adenine DNA glycosylase I, constitutive (tag),
dCMP phosphohydrolase (yfbR),
glycoside hydrolase (ygjK),
GTPase that interacts with methylmalonyl-CoA mutase (argK),
ribosome small subunit-dependent GTPase A (rsgA),
accessory protein for nickel incorporation into hydrogenase isoenzymes (hypB),
CDP-diacylglycerol pyrophosphatase (cdh),
purine nucleotidase (yrfG),
ATP-dependent RNA helicase, specific for 23S rRNA (dbpA),
formyltetrahydrofolate deformylase (purU),
broad specificity 5'(3')-nucleotidase and polyphosphatase (umpG),
meso-diaminopimelic acid-D-alanyl endopeptidase / D-alanyl-D-alanine carboxypeptidase (dacB),
penicillin-binding protein 7 (pbpG),
DD-endopeptidase / DD-carboxypeptidase (ampH),
murein DD-endopeptidase MepM,
murein DD-endopeptidase MepH,
murein DD-endopeptidase / murein LD-carboxypeptidase (mepS),
RNase HI, degrades RNA of DNA-RNA hybrids, participates in DNA replication (rnhA),
RNAse HII, degrades RNA of DNA-RNA hybrids (rnhB),
nucleoside triphosphate pyrophosphohydrolase (mazG),
ITPase/XTPase (yjjX),
glycerophosphodiester phosphodiesterase, cytosolic (ugpQ),
aminopeptidase N (pepN),
AphA monomer,
RNA pyrophosphohydrolase (rppH),
RNase E (rne),
6-phospho-β-glucosidase A (bglA),
RNase R (rnr),
exonuclease III (xthA),
nucleoside di- and triphosphate hydrolase [multifunctional] (nudJ),
pyridoxal phosphatase / HMP-PP hydrolase (cof),
fused tRNA nucleotidyltransferase / 2',3'-cyclic phosphodiesterase / 2' nucleotidase and phosphatase (cca),
2-deoxyglucose-6-phosphatase (yniC),
purine nucleoside phosphoramidase monomer (hinT),
deubiquitinase (elaD),
α-xylosidase (yicI),
intramembrane serine protease GlpG,
peptidase component of the HslVU protease,
2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase (cpdB),
EntB monomer,
ChpB toxin of the ChpB-ChpS toxin-antitoxin system,
ribonuclease II (rnb),
xylose ABC transporter - ATP binding subunit (xylG),
taurine ABC transporter - ATP binding subunit (tauB),
ABC complex for formation and release of holoCcmE - ATP binding subunit (ccmA),
arabinose ABC transporter - ATP binding subunit (araG),
glutathione ABC transporter - ATP binding subunit (gsiA),
phosphate ABC transporter - ATP binding subunit (pstB),
D-allose ABC transporter - ATP binding subunit (alsA),
Zn2+ ABC transporter - ATP binding subunit (znuC),
glycine betaine ABC transporter - putative ATP binding subunit (yehX),
ATP synthase F1 complex - alpha subunit (atpA),
ATP synthase F1 complex - beta subunit (atpD),
Cu+ efflux ATPase (copA),
molybdate ABC transporter - ATP binding subunit (modC),
L-arginine ABC transporter - ATP binding subunit (artP),
AI-2 ABC transporter - ATP binding subunit (lsrA),
K+ transporting ATPase - ATP binding subunit (kdpB),
phosphonate ABC transporter - ATP binding subunit (phnC),
maltose ABC transporter - ATP binding subunit (malK),
aliphatic sulfonate ABC transporter - ATP binding subunit (ssuB),
glycerol-3-phosphate ABC transporter - ATP binding subunit (ugpC),
zinc, cadmium and lead efflux system (zntA),
L-methionine / D-methionine ABC transporter - ATP binding subunit (metN),
L-cystine ABC transporter - predicted ATP binding subunit (tcyN),
galactose ABC transporter - ATP binding subunit (mglA),
nickel ABC transporter - ATP binding subunit (nikE),
nickel ABC transporter - ATP binding subunit (nikD),
sulfate / thiosulfate ABC transporter - ATP binding subunit (cysA),
putrescine / spermidine ABC transporter - ATP binding subunit (potA),
ATP-binding lipopolysaccharide transport protein (msbA),
ribose ABC transporter - putative ATP binding subunit (rbsA),
vitamin B12 ABC transporter - ATP binding subunit (btuD),
iron (III) hydroxamate ABC transporter - ATP binding subunit (fhuC),
2-oxoglutaramte:glutamine aminotransferase (mtnU),
2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX),
Dihydropyrimidinase (DPYS),
glycosylasparaginase beta chain (AGA),
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 (PFKFB2),
Fructose-2,6-bisphosphatase (PFKFB4),
Fructose-2,6-bisphosphatase (PFKFB3),
Beta-Ala-His dipeptidase (CNDP1),
Fumarylacetoacetase (FAH),
6-phosphogluconolactonase (PGLS),
Arginase-1 (ARG1),
Arginase-2, mitochondrial (ARG2),
bifunctional purine biosynthesis protein purh (ATIC),
kanosamine-6-phosphate phosphatase (ntdB),
V-type proton ATPase subunit S1 (ATP6AP1),
H+-translocating V-ATPase subunit G3 (ATP6V1G3),
H+-translocating V-ATPase subunit G2 (ATP6V1G2),
H+-translocating V-ATPase subunit B1 (ATP6V1B1),
H+-translocating V-ATPase subunit H (ATP6V1H),
H+-translocating V-ATPase subunit G1 (ATP6V1G1),
H+-translocating V-ATPase subunit F (ATP6V1F),
H+-translocating V-ATPase subunit E1 (ATP6V1E1),
H+-translocating V-ATPase subunit D (ATP6V1D),
H+-translocating V-ATPase subunit C1 (ATP6V1C1),
H+-translocating V-ATPase subunit B2 (ATP6V1B2),
H+-translocating V-ATPase subunit A (ATP6V1A),
H+-translocating V-ATPase subunit e1 (ATP6V0E1),
H+-translocating V-ATPase subunit c'' (ATP6V0B),
H+-translocating V-ATPase subunit d1 (ATP6V0D1),
glucose-6-phosphatase (G6PC),
fructose-1,6-bisphosphatase 2 (FBP2),
fructose-1,6-bisphosphatase 1 (FBP1),
phosphoglycerate mutase 2 (PGAM2),
Bisphosphoglycerate mutase (BPGM),
3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (iolD),
phosphoglycerate mutase 1 (PGAM1),
nucleoside triphosphate phosphohydrolase (ytkD),
cytidine/deoxycytidine deaminase monomer (cdd),
deoxycytidylate deaminase monomer (DCTD),
mitochondrial deoxyuridine 5'-triphosphate nucleotidohydrolase monomer (DUT),
cytidine deaminase monomer (CDA),
ectonucleoside triphosphate diphosphohydrolase 2 (ENTPD2),
ectonucleoside triphosphate diphosphohydrolase 4 (ENTPD4),
ectonucleoside triphosphate diphosphohydrolase 1 monomer (ENTPD1),
aminoacylase 2 monomer (ASPA),
aminoacylase 1 monomer (ACY1),
arabinogalactan endo-1,4-β-galactosidase (ganB),
8-oxo-dGTP diphosphatase / 2-hydroxy-dATP diphosphatase (MTH1),
mitochondrial glutaminase precursor (kidney isoform) (GLS),
N-formyl-4-amino-5-aminomethyl-2- methylpyrimidine deformylase (thiQ),
thiaminase II (tenA),
pancreatic triacylglycerol lipase (PNLIP),
liver carboxylesterase 1 monomer (CES1),
carboxylesterase 3 (CES3),
Dipeptidase 1 (DPEP1),
glucuronoarabinoxylan endo-1,4-β-xylanase (xynC),
α-L-arabinofuranosidase 2 (xsa),
α-L-arabinofuranosidase 1 (abfA),
arabinan-endo 1,5-α-L-arabinanase (abnA),
GTP cyclohydrolase I (folEA),
CAD protein,
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (bphD),
sphingomyelin phosphodiesterase 4 (Smpd4),
N-acylsphingosine amidohydrolase 1 (Asah1),
sphingomyelin phosphodiesterase 1, acid lysosomal (Smpd1),
sphingomyelin phosphodiesterase 3, neutral (Smpd3),
sphingomyelin phosphodiesterase 2, neutral (Smpd2),
sphingosine-1-phosphate phosphatase 1 (Sgpp1),
acyl-CoA thioesterase 2 (mitochondrial) (ACOT2)

Unification Links: GO:0016787

Relationship Links: ENZYME:RELATED-TO:3, Reactome:RELATED-TO:REACT_15331, Reactome:RELATED-TO:REACT_83734, Reactome:RELATED-TO:REACT_84047, Reactome:RELATED-TO:REACT_87959, Reactome:RELATED-TO:REACT_88159, Reactome:RELATED-TO:REACT_90118, Reactome:RELATED-TO:REACT_91045, Reactome:RELATED-TO:REACT_98572, Reactome:RELATED-TO:REACT_110436, Reactome:RELATED-TO:REACT_111159

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.5 on Sun Nov 29, 2015, BIOCYC13A.