Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: carotenoid isomerase

Gene: CRTISO Accession Number: AT1G06820 (MetaCyc)

Species: Arabidopsis thaliana col

Summary:
Mutants affected in the Arabidopsis thaliana CRTISO gene accumulate acyclic, poly-cis-carotene precursors: prolycopene, 7,9,9'-cis-neurosporene and a mix of ζ-carotene isomers [Park02]. The accumulation of these compounds leads to a distinctive orange-yellow coloration of the cotyledons, in constrast with the yellow coloration of those of wild type. The accumulation of cis-carotenes rapidly disappears upon exposure to light. The protein responsible for this mutant phenotype was identified as a carotenoid isomerase. Its activity was confirmed by heterologous expression in Escherichia coli: extracts of mutant, dark-grown Arabidopsis mutants were co-incubated with recombinant CRTISO-expressing Escherichia coli extracts; the mixture was shown to consistently contain an increase in all-trans-lycopene, all-trans neurosporene and all-trans-ζ-carotene [Park02].

Unification Links: Entrez-gene:CRTISO , Entrez:NP_172167 , Mint:MINT-8067289 , PhylomeDB:Q9M9Y8 , Pride:Q9M9Y8 , Protein Model Portal:Q9M9Y8 , SMR:Q9M9Y8 , String:3702.AT1G06820.1-P , TAIR:AT1G06820 , UniProt:Q9M9Y8

Relationship Links: Entrez-Nucleotide:PART-OF:NM_100559 , InterPro:IN-FAMILY:IPR014101 , Panther:IN-FAMILY:PTHR10668:SF30 , Pfam:IN-FAMILY:PF00890

Gene-Reaction Schematic: ?


Enzymatic reaction of: 7',9'-cis-lycopene isomerase (carotenoid isomerase)

EC Number: 5.2.1.-

7',9'-cis-lycopene <=> all-trans-lycopene

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.

Summary:
Although this activity has not been identified through direct experimentation using 7',9'-cis-lycopene as a substrate, CRTISO is hypothesized to catalyze this reaction as suggested for a comparable enzyme in tomato [Isaacson04].


Enzymatic reaction of: 7,9,9'-cis-neurosporene isomerase (carotenoid isomerase)

EC Number: 5.2.1.-

7,9,9'-cis-neurosporene <=> 9'-cis-neurosporene

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.

Summary:
Although this activity has not been identified through direct experimentation using 7,9,9'-cis-neurosporene as a substrate, CRTISO is hypothesized to catalyze this reaction as suggested for a comparable enzyme in tomato [Isaacson04].


Enzymatic reaction of: prolycopene isomerase (carotenoid isomerase)

EC Number: 5.2.1.13

prolycopene <=> all-trans-lycopene

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of carotenoid biosynthesis , trans-lycopene biosynthesis II (plants)


References

Isaacson04: Isaacson T, Ohad I, Beyer P, Hirschberg J (2004). "Analysis in vitro of the enzyme CRTISO establishes a poly-cis-carotenoid biosynthesis pathway in plants." Plant Physiol 136(4);4246-55. PMID: 15557094

Park02: Park H, Kreunen SS, Cuttriss AJ, DellaPenna D, Pogson BJ (2002). "Identification of the carotenoid isomerase provides insight into carotenoid biosynthesis, prolamellar body formation, and photomorphogenesis." Plant Cell 14(2);321-32. PMID: 11884677


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Dec 17, 2014, BIOCYC14B.