Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

MetaCyc Enzyme: phytoene synthase

Gene: PSY Accession Number: AT5G17230 (MetaCyc)

Synonyms: AT5G17230

Species: Arabidopsis thaliana col

Summary:
Phytoene synthase (PSY) catalyzes the committed step to carotenoids. The enzyme converts geranylgeranyl diphosphate, produced by the upstream methylerythritol phosphate pathway I and geranylgeranyl pyrophosphate synthase, to 15-cis-phytoene.

A cDNA encoding phytoene synthase (PSY) was isolated from Arabidopsis thaliana [Scolnik94] based on the PSY1 cDNA of tomato. Phytoene synthase catalyzes the dimerization of two molecules of geranylgeranyl pyrophosphate to phytoene. The PSY gene was overexpressed in Arabidopsis seedlings resulting in a significant increase of one the phytoene successor intermediate β-carotene [Lindgren03]. It has been demonstrated in phytochrome mutants of Arabidopsis thaliana that the expression of PSY is under the control of phytochrome [vonLintig97].
phytoene synthase (gb|AAB65697.1)

Gene Citations: [Bartley99]

Unification Links: ArrayExpress:P37271 , Entrez:AAA32836 , PhylomeDB:P37271 , Pride:P37271 , Protein Model Portal:P37271 , SMR:P37271 , String:3702.AT5G17230.3-P , TAIR:AT5g17230 , UniProt:P37271

Relationship Links: Entrez-Nucleotide:PART-OF:L25812 , InterPro:IN-FAMILY:IPR002060 , InterPro:IN-FAMILY:IPR008949 , InterPro:IN-FAMILY:IPR019845 , Pfam:IN-FAMILY:PF00494 , Prosite:IN-FAMILY:PS01044 , Prosite:IN-FAMILY:PS01045

Gene-Reaction Schematic: ?


Enzymatic reaction of: phytoene synthase

prephytoene diphosphate <=> 15-cis-phytoene + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: superpathway of carotenoid biosynthesis , trans-lycopene biosynthesis II (plants)


Enzymatic reaction of: prephytoene synthase

2 geranylgeranyl diphosphate <=> prephytoene diphosphate + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: superpathway of carotenoid biosynthesis , trans-lycopene biosynthesis II (plants)


References

Bartley99: Bartley GE, Scolnik PA, Beyer P (1999). "Two Arabidopsis thaliana carotene desaturases, phytoene desaturase and zeta-carotene desaturase, expressed in Escherichia coli, catalyze a poly-cis pathway to yield pro-lycopene." Eur J Biochem 1999;259(1-2);396-403. PMID: 9914519

Lindgren03: Lindgren LO, Stalberg KG, Hoglund AS (2003). "Seed-specific overexpression of an endogenous Arabidopsis phytoene synthase gene results in delayed germination and increased levels of carotenoids, chlorophyll, and abscisic acid." Plant Physiol 132(2);779-85. PMID: 12805607

Scolnik94: Scolnik PA, Bartley GE (1994). "Nucleotide sequence of an Arabidopsis cDNA for phytoene synthase." Plant Physiol 104(4);1471-2. PMID: 8016277

vonLintig97: von Lintig J, Welsch R, Bonk M, Giuliano G, Batschauer A, Kleinig H (1997). "Light-dependent regulation of carotenoid biosynthesis occurs at the level of phytoene synthase expression and is mediated by phytochrome in Sinapis alba and Arabidopsis thaliana seedlings." Plant J 12(3);625-34. PMID: 9351247


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 21, 2014, BIOCYC14A.