MetaCyc Enzyme: 7-carboxy-7-deazaguanine synthase

Gene: queE Accession Number: BSU13740 (MetaCyc)

Synonyms: ykvL

Species: Bacillus subtilis subtilis 168

The queC, queD, queE, and queF genes of Bacillus subtilis were implicated by comparative genomics as candidates in the biosynthetic pathway of preQ1. Deletions of these genes resulted in mutants that did not contain queuosine in their tRNAs [Reader04, deCrecyLagard07].

A BLAST analysis of QueE showed that it belongs to the family of S-adenosyl-L-methionine (SAM)-dependent organic radical-generating enzymes. These enzymes contain a [4Fe-4S] cluster, which when reductively activated cleaves SAM to 5'-deoxyadenosyl radical, which in turn initiates radical-mediated transformations of the substrate.

The queE gene of Bacillus subtilis was cloned and expressed as a His-tagged fusion protein in a strain of Escherichia coli optimized for expression of iron-sulfur cluster-containing enzymes. The Recombinant QueE was purified under strictly anaerobic conditions and used within a few hours of purification to preserve the [4Fe-4S] cluster. The product of the purified enzyme was shown to be 7-carboxy-7-deazaguanine [McCarty09].

Map Position: [1,440,542 -> 1,441,273]

Molecular Weight of Polypeptide: 27.122 kD (from nucleotide sequence)

Unification Links: Entrez:2633745 , GenoList (SubtiList):BSU13740 , GOA:O31677 , Protein Model Portal:O31677 , String:224308.BSU13740 , String:BSU13740 , SubtilisWiki:queE , SubtiWiki:queE , UniProt:O31677

Relationship Links: InterPro:IN-FAMILY:IPR007197 , InterPro:IN-FAMILY:IPR013785 , InterPro:IN-FAMILY:IPR017742 , InterPro:IN-FAMILY:IPR024924 , Pfam:IN-FAMILY:PF04055

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0008616 - queuosine biosynthetic process Inferred by computational analysis [GOA00]
Molecular Function: GO:0003824 - catalytic activity Inferred by computational analysis [GOA01a]
GO:0016829 - lyase activity Inferred by computational analysis [GOA00]
GO:0046872 - metal ion binding Inferred by computational analysis [GOA00]
GO:0051536 - iron-sulfur cluster binding Inferred by computational analysis [GOA00, GOA01a]
GO:0051539 - 4 iron, 4 sulfur cluster binding Inferred by computational analysis [GOA00]


Created 24-Jan-2011 by Caspi R , SRI International

Enzymatic reaction of: 7-carboxy-7-deazaguanine synthase

EC Number:

6-carboxy-5,6,7,8-tetrahydropterin + H+ <=> 7-carboxy-7-deazaguanine + ammonium

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: preQ0 biosynthesis

Cofactors or Prosthetic Groups: a [4Fe-4S] iron-sulfur cluster [McCarty09]


deCrecyLagard07: de Crecy-Lagard V (2007). "Identification of genes encoding tRNA modification enzymes by comparative genomics." Methods Enzymol 425;153-83. PMID: 17673083

GOA00: GOA (2000). "Gene Ontology annotation based on Swiss-Prot keyword mapping."

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

McCarty09: McCarty RM, Somogyi A, Lin G, Jacobsen NE, Bandarian V (2009). "The deazapurine biosynthetic pathway revealed: in vitro enzymatic synthesis of PreQ(0) from guanosine 5'-triphosphate in four steps." Biochemistry 48(18);3847-52. PMID: 19354300

Reader04: Reader JS, Metzgar D, Schimmel P, de Crecy-Lagard V (2004). "Identification of four genes necessary for biosynthesis of the modified nucleoside queuosine." J Biol Chem 279(8);6280-5. PMID: 14660578

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sun Oct 4, 2015, biocyc13.