|Gene:||queE||Accession Number: BSU13740 (MetaCyc)|
Species: Bacillus subtilis subtilis 168
The queC, queD, queE, and queF genes of Bacillus subtilis were implicated by comparative genomics as candidates in the biosynthetic pathway of preQ1. Deletions of these genes resulted in mutants that did not contain queuosine in their tRNAs [Reader04, deCrecyLagard07].
A BLAST analysis of QueE showed that it belongs to the family of S-adenosyl-L-methionine (SAM)-dependent organic radical-generating enzymes. These enzymes contain a [4Fe-4S] cluster, which when reductively activated cleaves SAM to 5'-deoxyadenosyl radical, which in turn initiates radical-mediated transformations of the substrate.
The queE gene of Bacillus subtilis was cloned and expressed as a His-tagged fusion protein in a strain of Escherichia coli optimized for expression of iron-sulfur cluster-containing enzymes. The Recombinant QueE was purified under strictly anaerobic conditions and used within a few hours of purification to preserve the [4Fe-4S] cluster. The product of the purified enzyme was shown to be 7-carboxy-7-deazaguanine [McCarty09a].
|Map Position: [1,440,542 -> 1,441,273]|
Molecular Weight of Polypeptide: 27.122 kD (from nucleotide sequence)
Unification Links: Entrez:2633745 , GenoList (SubtiList):BSU13740 , GOA:O31677 , Protein Model Portal:O31677 , String:224308.BSU13740 , String:BSU13740 , SubtilisWiki:queE , SubtiWiki:queE , UniProt:O31677
|Biological Process:||GO:0008616 - queuosine biosynthetic process [GOA00]|
|Molecular Function:||GO:0003824 - catalytic activity
GO:0016829 - lyase activity [GOA00]
GO:0046872 - metal ion binding [GOA00]
GO:0051536 - iron-sulfur cluster binding [GOA00, GOA01a]
GO:0051539 - 4 iron, 4 sulfur cluster binding [GOA00]
Enzymatic reaction of: 7-carboxy-7-deazaguanine synthase
EC Number: 126.96.36.199
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: preQ0 biosynthesis
McCarty09a: McCarty RM, Somogyi A, Lin G, Jacobsen NE, Bandarian V (2009). "The deazapurine biosynthetic pathway revealed: in vitro enzymatic synthesis of PreQ(0) from guanosine 5'-triphosphate in four steps." Biochemistry 48(18);3847-52. PMID: 19354300
Reader04: Reader JS, Metzgar D, Schimmel P, de Crecy-Lagard V (2004). "Identification of four genes necessary for biosynthesis of the modified nucleoside queuosine." J Biol Chem 279(8);6280-5. PMID: 14660578
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