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MetaCyc Polypeptide: chitobiose PTS permease - ChbB subunit

Gene: chbB Accession Numbers: EG10140 (MetaCyc), b1738, ECK1736

Synonyms: celA, Enzyme IIBcel, EIIBcel, EIIchb, Enzyme IIBchb

Species: Escherichia coli K-12 substr. MG1655

Component of: chitobiose PTS permease (extended summary available)

Summary:
ChbB contains a PTS Enzyme IIB domain. Cys10 is the site of phosphotransfer during the PTScbh reaction sequence [Keyhani00].

The 3-dimensional structure of IIBChb has been determined by NMR spectroscopy [Ab94, Ab97, Ab01] and by X-ray crystallography to a resolution of 1.8 Å [vanMontfort94, vanMontfort97]. A solution structure of a ChbB-ChbA complex has been obtained and the interaction sites mapped. The protruding active site of ChbB containing the active site cysteine residue (Cys10) is buried within the cleft formed by the adjacent subunits of the ChbA trimer [Jung10a].

chbB: N,N'-diacetylchitobiose B

Locations: cytosol

Map Position: [1,819,323 <- 1,819,643]

Molecular Weight of Polypeptide: 11.427 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0005797 , CGSC:34873 , EchoBASE:EB0138 , EcoGene:EG10140 , EcoliWiki:b1738 , ModBase:P69795 , OU-Microarray:b1738 , PortEco:chbB , PR:PRO_000022273 , Protein Model Portal:P69795 , RefSeq:NP_416252 , RegulonDB:EG10140 , SMR:P69795 , String:511145.b1738 , UniProt:P69795

Relationship Links: InterPro:IN-FAMILY:IPR003501 , InterPro:IN-FAMILY:IPR013012 , PDB:Structure:1E2B , PDB:Structure:1H9C , PDB:Structure:1IIB , PDB:Structure:2WWV , PDB:Structure:2WY2 , Pfam:IN-FAMILY:PF02302 , Prosite:IN-FAMILY:PS51100

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA01, Keyhani00a, Keyhani00, Reizer90]
GO:0034219 - carbohydrate transmembrane transport Inferred from experiment Inferred by computational analysis [Reizer90, Keyhani00, GOA01a, GOA01]
GO:1902815 - N,N'-diacetylchitobiose import Inferred from experiment [Keyhani00a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Keyhani00b]
GO:0090566 - protein-phosphocysteine-N,N'-diacetylchitobiose phosphotransferase system transporter activity Inferred by computational analysis Inferred from experiment [Keyhani00, Reizer90]
GO:0008982 - protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred by computational analysis [GOA01a, GOA01]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Keyhani00b]
GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]

MultiFun Terms: metabolism carbon utilization carbon compounds
transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Subunit of: chitobiose PTS permease

Synonyms: EIIcel, EIIchb, Enzyme IIcel, Enzyme II chb

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of chitobiose PTS permease = [ChbA][ChbC][ChbB]
         chitobiose PTS permease - ChbA subunit = ChbA (extended summary available)
         chitobiose PTS permease - ChbC subunit = ChbC (summary available)
         chitobiose PTS permease - ChbB subunit = ChbB (summary available)

Summary:
ChbBCA belongs to the functional superfamily of the PEP-dependent, sugar transporting phosphotransferase system (PTSsugar). The N,N'-diacetylchitobiose (chitobiose) PTS transporter takes up exogenous chitobiose, the β1-4 linked dimer of N-acetylglucosamine and major breakdown product of chitin, releasing the phosphate ester into the cell cytoplasm in preparation for hydrolysis and metabolism, primarily via glycolysis [Keyhani97]. Diacetylchitobiose, triacetylchitobiose and the non-metabolizable analogue methyl β-diacetylthiochitobiose are all inducers and substrates of the chitobiose permease [Keyhani00a].

The chitobiose PTS transporter requires the general PTS enzymes Enzyme I (PtsI) and HPr (PtsH) as well as Enzyme IIchb for transport and phosphorylation of its substrates [Keyhani00a]. The Enzyme IIchb complex, possesses three polypeptide chains: ChbA, ChbB, and ChbC [Parker90, Reizer90]. It is homologous to the well-characterized lactose-specific PTS Enzyme II of Gram-positive bacteria [Reizer90]. ChbC is an integral membrane transport protein while IIA (ChbA) and IIB (ChbB) are soluble [Reizer90, Keyhani00b]. Enzyme IIchb mediates a phosphotransfer and transport reaction whereby a phosphoryl group is transferred from phospho-HPr to ChbA, from ChbA to ChbB and then onto the incoming sugar substrate [Keyhani00a, Keyhani00c, Keyhani00]. ChbCBA is a member of the PTS Lactose - N,N'-diacetylchitobiose-β-glycoside (Lac) family of transporters [Saier14].

E. coli is chemotactic towards chitobiose [Keyhani97].

The chb operon contains in addition to chbBCA, the chbR gene, which encodes a negative regulatory protein, the chbF gene encoding an enzyme that functions as an acetylchitobiose-6-phosphate hydrolase and the chbG encoding a chitooligosaccharide deacetylase. The operon gene order is chbBCARFG. The chb operon is subject to complex regulation by three transcription factors: ChbR, NagC and cyclic AMP-cyclic AMP receptor protein complex [Plumbridge04].

Mutations can occur within the chb system which allow the uptake of the β-glucosides arbutin, salicin, and cellobiose. Such mutations include integration of insertion sequences upstream of the transcriptional start site and point mutations of the ChbR repressor that allow recognition of these β-glucosides as inducers [Kricker84, Hall86, Kricker87, Hall87, Hall87a, Parker90]. Cellobiose positive strains contain mutations that alter the regulation of chb genes. Specifically the mutations result in loss of NagC repression and gain of function in ChbR. This suggests that the wild-type permease can recognize and transport cellobiose [Kachroo07]

chb: N,N'-diacetylchitobiose

Citations: [Parker90a]

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from experiment Inferred by computational analysis [Reizer90, Keyhani00a, Keyhani00]
GO:1902815 - N,N'-diacetylchitobiose import Inferred from experiment [Keyhani97, Keyhani00a]
Molecular Function: GO:0090566 - protein-phosphocysteine-N,N'-diacetylchitobiose phosphotransferase system transporter activity Inferred from experiment Inferred by computational analysis [Reizer90, Keyhani00, Keyhani00a]

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: transport and phosphorylation of chitobiose (chitobiose PTS permease)

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International

Summary:
The Km for uptake of the non-metabolizable analogue methyl β-diacetylthiochitobiose is 50-100µM [Keyhani00a]


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 3 -> 106
[UniProt09]
UniProt: PTS EIIB type-3;
Active-Site 10
[UniProt10]
UniProt: Phosphocysteine intermediate; Non-Experimental Qualifier: by similarity;

History:
10/20/97 Gene b1738 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10140; confirmed by SwissProt match.


References

Ab01: Ab E, Schuurman-Wolters GK, Nijlant D, Dijkstra K, Saier MH, Robillard GT, Scheek RM (2001). "NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli." J Mol Biol 308(5);993-1009. PMID: 11352587

Ab94: Ab E, Schuurman-Wolters GK, Saier MH, Reizer J, Jacuinod M, Roepstorff P, Dijkstra K, Scheek RM, Robillard GT (1994). "Enzyme IIBcellobiose of the phosphoenol-pyruvate-dependent phosphotransferase system of Escherichia coli: backbone assignment and secondary structure determined by three-dimensional NMR spectroscopy." Protein Sci 3(2);282-90. PMID: 8003964

Ab97: Ab E, Schuurman-Wolters G, Reizer J, Saier MH, Dijkstra K, Scheek RM, Robillard GT (1997). "The NMR side-chain assignments and solution structure of enzyme IIBcellobiose of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli." Protein Sci 6(2);304-14. PMID: 9041631

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Hall86: Hall BG, Betts PW, Kricker M (1986). "Maintenance of the cellobiose utilization genes of Escherichia coli in a cryptic state." Mol Biol Evol 3(5);389-402. PMID: 2832693

Hall87: Hall BG, Betts PW (1987). "Cryptic genes for cellobiose utilization in natural isolates of Escherichia coli." Genetics 115(3);431-9. PMID: 3552874

Hall87a: Hall BG, Faunce W (1987). "Functional genes for cellobiose utilization in natural isolates of Escherichia coli." J Bacteriol 169(6);2713-7. PMID: 3034866

Jung10a: Jung YS, Cai M, Clore GM (2010). "Solution structure of the IIAChitobiose-IIBChitobiose complex of the N,N'-diacetylchitobiose branch of the Escherichia coli phosphotransferase system." J Biol Chem 285(6);4173-84. PMID: 19959833

Kachroo07: Kachroo AH, Kancherla AK, Singh NS, Varshney U, Mahadevan S (2007). "Mutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose." Mol Microbiol 66(6);1382-95. PMID: 18028317

Keyhani00: Keyhani NO, Bacia K, Roseman S (2000). "The transport/phosphorylation of N,N'-diacetylchitobiose in Escherichia coli. Characterization of phospho-IIB(Chb) and of a potential transition state analogue in the phosphotransfer reaction between the proteins IIA(Chb) AND IIB(Chb)." J Biol Chem 275(42);33102-9. PMID: 10913119

Keyhani00a: Keyhani NO, Wang LX, Lee YC, Roseman S (2000). "The chitin disaccharide, N,N'-diacetylchitobiose, is catabolized by Escherichia coli and is transported/phosphorylated by the phosphoenolpyruvate:glycose phosphotransferase system." J Biol Chem 275(42);33084-90. PMID: 10913117

Keyhani00b: Keyhani N, Rodgers ME, Demeler B, Hansen JC, Roseman S (2000). "Analytical sedimentation of the IIAChb and IIBChb proteins of the Escherichia coli N,N'-diacetylchitobiose phosphotransferase system. Demonstration of a model phosphotransfer transition state complex." J Biol Chem 275(42);33110-5. PMID: 10913122

Keyhani00c: Keyhani NO, Boudker O, Roseman S (2000). "Isolation and characterization of IIAChb, a soluble protein of the enzyme II complex required for the transport/phosphorylation of N, N'-diacetylchitobiose in Escherichia coli." J Biol Chem 275(42);33091-101. PMID: 10913118

Keyhani97: Keyhani NO, Roseman S (1997). "Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon." Proc Natl Acad Sci U S A 1997;94(26);14367-71. PMID: 9405618

Kricker84: Kricker M, Hall BG (1984). "Directed evolution of cellobiose utilization in Escherichia coli K12." Mol Biol Evol 1(2);171-82. PMID: 6400650

Kricker87: Kricker M, Hall BG (1987). "Biochemical genetics of the cryptic gene system for cellobiose utilization in Escherichia coli K12." Genetics 115(3);419-29. PMID: 3552873

Parker90: Parker LL, Hall BG (1990). "Mechanisms of activation of the cryptic cel operon of Escherichia coli K12." Genetics 1990;124(3);473-82. PMID: 2179048

Parker90a: Parker LL, Hall BG (1990). "Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12." Genetics 124(3);455-71. PMID: 2179047

Plumbridge04: Plumbridge J, Pellegrini O (2004). "Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP." Mol Microbiol 52(2);437-49. PMID: 15066032

Reizer90: Reizer J, Reizer A, Saier MH (1990). "The cellobiose permease of Escherichia coli consists of three proteins and is homologous to the lactose permease of Staphylococcus aureus." Res Microbiol 1990;141(9);1061-7. PMID: 2092358

Saier14: Saier MH, Reddy VS, Tamang DG, Vastermark A (2014). "The transporter classification database." Nucleic Acids Res 42(Database issue);D251-8. PMID: 24225317

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

vanMontfort94: van Montfort RL, Pijning T, Kalk KH, Schuurman-Wolters GK, Reizer J, Saier MH, Robillard G, Dijkstra BW (1994). "Crystallization of enzyme IIB of the cellobiose-specific phosphotransferase system of Escherichia coli." J Mol Biol 239(4);588-90. PMID: 8006971

vanMontfort97: van Montfort RL, Pijning T, Kalk KH, Reizer J, Saier MH, Thunnissen MM, Robillard GT, Dijkstra BW (1997). "The structure of an energy-coupling protein from bacteria, IIBcellobiose, reveals similarity to eukaryotic protein tyrosine phosphatases." Structure 1997;5(2);217-25. PMID: 9032081


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc13.