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MetaCyc Polypeptide: Cytochrome b6

Gene: petB Accession Number: CHRECP008 (MetaCyc)

Species: Chlamydomonas reinhardtii

Component of:
Cyt b6f monomer
Cyt b6f complex (summary available)

Summary:
PetB is one the large subunits of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI). It binds 2 heme groups.

Citations: [deVitry04, Stroebel03, Maul02, Zito97, Pierre95, Buschlen91, Huang92a]

Locations: chloroplast, chloroplast thylakoid membrane

Map Position: [20,007 <- 20,654]

Molecular Weight of Polypeptide: 24.16 kD (from nucleotide sequence)

Unification Links: DIP:DIP-58593N , Entrez-gene:2717017 , Pride:Q00471 , Protein Model Portal:Q00471 , RefSeq:NP_958365.1 , SMR:Q00471 , UniProt:Q00471

Relationship Links: InterPro:IN-FAMILY:IPR005797 , InterPro:IN-FAMILY:IPR016174 , InterPro:IN-FAMILY:IPR016175 , InterPro:IN-FAMILY:IPR023530 , InterPro:IN-FAMILY:IPR027387 , Panther:IN-FAMILY:PTHR19271:SF0 , PDB:Structure:1Q90 , Pfam:IN-FAMILY:PF13631 , Prosite:IN-FAMILY:PS51002 , RefSeq:PART-OF:NC_005353.1

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0009507 - chloroplast
GO:0042651 - thylakoid membrane

MultiFun Terms: metabolism energy production/transport

Credits:
Created 12-Jan-2010 by May P , MPI-MP
Revised 04-May-2011 by Weerasinghe D


Subunit of: Cyt b6f monomer

Species: Chlamydomonas reinhardtii

Subunit composition of Cyt b6f monomer = [PetC][PetM][PetN][PetL][PetG][PetD][PetB][PetA]
         Cytochrome b6f complex iron-sulfur subunit = PetC (summary available)
         Cytochrome b6f complex subunit 7 = PetM (summary available)
         Cytochrome b6f complex subunit 8 = PetN (summary available)
         Cytochrome b6f complex subunit 6 = PetL (summary available)
         Cytochrome b6f complex subunit 5 = PetG (summary available)
         Cytochrome b6f complex subunit 4 = PetD (summary available)
         Cytochrome b6 = PetB (summary available)
         apocytochrome f = PetA (summary available)

Component of: Cyt b6f complex (summary available)

Locations: chloroplast, chloroplast thylakoid membrane

GO Terms:

Cellular Component: GO:0009507 - chloroplast
GO:0042651 - thylakoid membrane

Credits:
Created 12-Jan-2010 by May P , MPI-MP


Subunit of: Cyt b6f complex

Species: Chlamydomonas reinhardtii

Subunit composition of Cyt b6f complex = [(PetC)(PetM)(PetN)(PetL)(PetG)(PetD)(PetB)(PetA)]2
         Cyt b6f monomer = (PetC)(PetM)(PetN)(PetL)(PetG)(PetD)(PetB)(PetA)
                 Cytochrome b6f complex iron-sulfur subunit = PetC (summary available)
                 Cytochrome b6f complex subunit 7 = PetM (summary available)
                 Cytochrome b6f complex subunit 8 = PetN (summary available)
                 Cytochrome b6f complex subunit 6 = PetL (summary available)
                 Cytochrome b6f complex subunit 5 = PetG (summary available)
                 Cytochrome b6f complex subunit 4 = PetD (summary available)
                 Cytochrome b6 = PetB (summary available)
                 apocytochrome f = PetA (summary available)

Summary:
Cytochrome b6f is a plastohydroquinone:plastocyanin oxidoreductase present in chloroplasts and plays a major role in the photosynthetic electron transport chain [Kuras07]. It contributes to the proton motive force via the Q-cycle [Malnoe11]. The Q-cycle is a chemic process for proton translocation from the chloroplast stroma into the lumen across the thylakoid membrane [Allen04]. Specifically it couples electron transfer with proton translocation to generate a transmembrane electrochemical H+ gradient [Stroebel03]. X-ray structure of cytochrome b6f has revealed the presence of an atypical heme [deLacroix09]. Furthermore, there is biochemical evidence to suggest that the cytochrome b6f complex contains within its folds a chlorophyll and β carotene molecule, though the function of these two pigments is not known [Pierre97][Zhang99].

Citations: [Catherine09, Baniulis]

Credits:
Created 12-Jan-2010 by May P , MPI-MP
Revised 19-May-2011 by Weerasinghe D


Enzymatic reaction of: plastoquinol-plastocyanin reductase (Cyt b6f complex)

EC Number: 1.10.9.1

In Pathways: photosynthesis light reactions , hydrogen production VIII

Citations: [Baniulis]


References

Allen04: Allen JF (2004). "Cytochrome b6f: structure for signalling and vectorial metabolism." Trends Plant Sci 9(3);130-7. PMID: 15003236

Baniulis: Baniulis D, Yamashita E, Zhang H, Hasan SS, Cramer WA "Structure-function of the cytochrome b6f complex." Photochem Photobiol 84(6);1349-58. PMID: 19067956

Buschlen91: Buschlen S, Choquet Y, Kuras R, Wollman FA (1991). "Nucleotide sequences of the continuous and separated petA, petB and petD chloroplast genes in Chlamydomonas reinhardtii." FEBS Lett 284(2);257-62. PMID: 2060646

Catherine09: Catherine de Vitry, Richard Kuras (2009). "The Cytochrome b6f Complex." Chapter 17. In: The Chlamydomonas Sourcebook. Second Edition.

deLacroix09: de Lacroix de Lavalette A, Barucq L, Alric J, Rappaport F, Zito F (2009). "Is the redox state of the ci heme of the cytochrome b6f complex dependent on the occupation and structure of the Qi site and vice versa?." J Biol Chem 284(31);20822-9. PMID: 19478086

deVitry04: de Vitry C, Desbois A, Redeker V, Zito F, Wollman FA (2004). "Biochemical and spectroscopic characterization of the covalent binding of heme to cytochrome b6." Biochemistry 43(13);3956-68. PMID: 15049703

Huang92a: Huang C, Liu XQ (1992). "Nucleotide sequence of the frxC, petB and trnL genes in the chloroplast genome of Chlamydomonas reinhardtii." Plant Mol Biol 18(5);985-8. PMID: 1581576

Hurt81: Hurt E, Hauska G (1981). "A cytochrome f/b6 complex of five polypeptides with plastoquinol-plastocyanin-oxidoreductase activity from spinach chloroplasts." Eur J Biochem 117(3);591-5. PMID: 6269845

Kuras07: Kuras R, Saint-Marcoux D, Wollman FA, de Vitry C (2007). "A specific c-type cytochrome maturation system is required for oxygenic photosynthesis." Proc Natl Acad Sci U S A 104(23);9906-10. PMID: 17535914

Malnoe11: Malnoe A, Wollman FA, de Vitry C, Rappaport F (2011). "Photosynthetic growth despite a broken Q-cycle." Nat Commun 2;301. PMID: 21556055

Maul02: Maul JE, Lilly JW, Cui L, dePamphilis CW, Miller W, Harris EH, Stern DB (2002). "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats." Plant Cell 14(11);2659-79. PMID: 12417694

Pierre95: Pierre Y, Breyton C, Kramer D, Popot JL (1995). "Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii." J Biol Chem 270(49);29342-9. PMID: 7493968

Pierre97: Pierre Y, Breyton C, Lemoine Y, Robert B, Vernotte C, Popot JL (1997). "On the presence and role of a molecule of chlorophyll a in the cytochrome b6 f complex." J Biol Chem 272(35);21901-8. PMID: 9268323

Stroebel03: Stroebel D, Choquet Y, Popot JL, Picot D (2003). "An atypical haem in the cytochrome b(6)f complex." Nature 426(6965);413-8. PMID: 14647374

Zhang99: Zhang H, Huang D, Cramer WA (1999). "Stoichiometrically bound beta-carotene in the cytochrome b6f complex of oxygenic photosynthesis protects against oxygen damage." J Biol Chem 274(3);1581-7. PMID: 9880536

Zito97: Zito F, Kuras R, Choquet Y, Kossel H, Wollman FA (1997). "Mutations of cytochrome b6 in Chlamydomonas reinhardtii disclose the functional significance for a proline to leucine conversion by petB editing in maize and tobacco." Plant Mol Biol 33(1);79-86. PMID: 9037161


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Dec 17, 2014, BIOCYC14B.