MetaCyc Enzyme: malate thiokinase

Synonyms: MTK, Malate--CoA ligase

Species: Methylobacterium extorquens AM1

Subunit composition of malate thiokinase = [MtkB][MtkA]
         malate thiokinase α subunit = MtkB
         malate thiokinase β subunit = MtkA

Malate thiokinase (MTK) has not been purified from M. extorquens. However, the genes encoding it were cloned, and mutants were constructed. The mutants were unable to grow on C1 compounds. Complementation of the mutants with the cloned genes restored MTK activity, verifying the correlation between the genes and MTK activity [Chistoserdova94b]. The protein is very similar to succinyl-CoA synthetase from Escherichia coli and Thermus thermophilus.

Gene-Reaction Schematic

Gene-Reaction Schematic

Enzymatic reaction of: malate thiokinase

Inferred from experiment

EC Number:

(S)-malate + ATP + coenzyme A ⇄ (S)-malyl-CoA + ADP + phosphate

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the opposite direction.

In Pathways: formaldehyde assimilation I (serine pathway), ethylmalonyl-CoA pathway

Subunit of malate thiokinase: malate thiokinase α subunit

Synonyms: MtkB

Gene: mtkB Accession Number: G-3285 (MetaCyc)

Unification Links: ModBase:P53595, Protein Model Portal:P53595, SMR:P53595, Swiss-Model:P53595, UniProt:P53595

Relationship Links: InterPro:IN-FAMILY:IPR003781, InterPro:IN-FAMILY:IPR005810, InterPro:IN-FAMILY:IPR005811, InterPro:IN-FAMILY:IPR016040, InterPro:IN-FAMILY:IPR016102, InterPro:IN-FAMILY:IPR017440, Pfam:IN-FAMILY:PF00549, Pfam:IN-FAMILY:PF02629, Prints:IN-FAMILY:PR01798, Prosite:IN-FAMILY:PS00399, Prosite:IN-FAMILY:PS01216, Smart:IN-FAMILY:SM00881

Subunit of malate thiokinase: malate thiokinase β subunit

Synonyms: MtkA

Gene: mtkA Accession Number: G-3284 (MetaCyc)

Unification Links: ModBase:P53594, Protein Model Portal:P53594, Swiss-Model:P53594, UniProt:P53594

Relationship Links: InterPro:IN-FAMILY:IPR005809, InterPro:IN-FAMILY:IPR005811, InterPro:IN-FAMILY:IPR011761, InterPro:IN-FAMILY:IPR013650, InterPro:IN-FAMILY:IPR013815, InterPro:IN-FAMILY:IPR013816, InterPro:IN-FAMILY:IPR016102, InterPro:IN-FAMILY:IPR017866, Panther:IN-FAMILY:PTHR11815, Pfam:IN-FAMILY:PF00549, Pfam:IN-FAMILY:PF08442, Prosite:IN-FAMILY:PS01217, Prosite:IN-FAMILY:PS50975


Chistoserdova94b: Chistoserdova LV, Lidstrom ME (1994). "Genetics of the serine cycle in Methylobacterium extorquens AM1: identification, sequence, and mutation of three new genes involved in C1 assimilation, orf4, mtkA, and mtkB." J Bacteriol 176(23);7398-404. PMID: 7961516

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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