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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: methenyl-H4MPT cyclohydrolase

Gene: mch Accession Number: G-353 (MetaCyc)

Species: Methanothermobacter thermautotrophicus

Subunit composition of methenyl-H4MPT cyclohydrolase = [Mch]2

Locations: cytosol

Molecular Weight of Polypeptide: 34.098 kD (from nucleotide sequence) [Bairoch, 1993 ]

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0005829 - cytosol


Enzymatic reaction of: methenyl-H4MPT cyclohydrolase

Synonyms: methenyltetrahydromethanopterin cyclohydrolase, N5,N10-methenyltetrahydromethanopterin cyclohydrolase, 5,10-methenyltetrahydromethanopterin 10-hydrolase (decyclizing)

EC Number: 3.5.4.27

5,10-methenyltetrahydromethanopterin + H2O <=> 5-formyl-tetrahydromethanopterin + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

In Pathways: superpathway of methanogenesis , methanogenesis from CO2

Summary:
Methenyl-H4MPT cyclohydrolase catalyzes the third reaction in methanogenesis from CO2. In the hydrolytic direction it produces a 5-formyl derivative as contrasted to the tetrahydrofolate enzymes which produce a 10-formyl derivative. [DiMarco86, Donnelly85, Vaupel96, Thauer98]

Activators (Unknown Mechanism): Mg2+ [DiMarco86]

Inhibitors (Unknown Mechanism): 10-formyltetrahydromethanopterin [DiMarco86] , Mn2+ [DiMarco86]


References

Bairoch, 1993: Bairoch A, Boeckmann B (1993). "The SWISS-PROT protein sequence data bank, recent developments." Nucleic Acids Res. 21:3093-3096. PMID: 8332529

DiMarco86: DiMarco AA, Donnelly MI, Wolfe RS (1986). "Purification and properties of the 5,10-methenyltetrahydromethanopterin cyclohydrolase from Methanobacterium thermoautotrophicum." J Bacteriol 1986;168(3);1372-7. PMID: 3782039

Donnelly85: Donnelly MI, Escalante-Semerena JC, Rinehart KL, Wolfe RS (1985). "Methenyl-tetrahydromethanopterin cyclohydrolase in cell extracts of Methanobacterium." Arch Biochem Biophys 1985;242(2);430-9. PMID: 4062290

Thauer98: Thauer RK (1998). "Biochemistry of methanogenesis: a tribute to Marjory Stephenson." Microbiology 1998;144 ( Pt 9);2377-406. PMID: 9782487

Vaupel96: Vaupel M, Dietz H, Linder D, Thauer RK (1996). "Primary structure of cyclohydrolase (Mch) from Methanobacterium thermoautotrophicum (strain Marburg) and functional expression of the mch gene in Escherichia coli." Eur J Biochem 1996;236(1);294-300. PMID: 8617278


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, BIOCYC14A.