Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: trimethylamine--corrinoid protein Co-methyltransferase

Gene: mttB Accession Numbers: G-9437 (MetaCyc), Mbar_A1502

Species: Methanosarcina barkeri

Subunit composition of trimethylamine--corrinoid protein Co-methyltransferase = [MttB]
         trimethylamine methyltransferase mttB subunit = MttB

Summary:
Reconstitution of trimethylamine-dependent coenzyme M (CoM) methylation showed that three polypeptides were involved - trimethylamine--corrinoid protein Co-methyltransferase, trimethylamine-specific corrinoid protein and methylated [methylamine-specific corrinoid protein]:coenzyme M methyltransferase.

The first two proteins, of sizes 52 and 26 kDa, respectively, copurified as a single trimethylamine methyltransferase (TMA-MT). Gel permeation of the TMA-MT fraction demonstrated that the 52- kDa polypeptide eluted with an apparent molecular mass of 280 kDa. The 26-kDa protein eluted primarily as a monomer, but some 26-kDa polypeptides also eluted with the 280-kDa peak, indicating that the two proteins weakly associate [Ferguson97].

Molecular Weight of Polypeptide: 53.855 kD (from nucleotide sequence)

Unification Links: Entrez-gene:3627689 , UniProt:O93658

Relationship Links: Entrez-Nucleotide:RELATED-TO:AF102623 , InterPro:IN-FAMILY:IPR010426 , InterPro:IN-FAMILY:IPR012740 , Pfam:IN-FAMILY:PF06253

Gene-Reaction Schematic: ?


Enzymatic reaction of: trimethylamine methyltransferase (trimethylamine--corrinoid protein Co-methyltransferase)

EC Number: 2.1.1.250

trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] + H+ <=> a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of methanogenesis , methanogenesis from trimethylamine


References

Ferguson97: Ferguson DJ, Krzycki JA (1997). "Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri." J Bacteriol 179(3);846-52. PMID: 9006042


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc14.