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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: (R,S)-reticuline 7-O-methyltransferase

Gene: 7OMT Accession Number: G-9902 (MetaCyc)

Synonyms: PSOMT1

Species: Papaver somniferum

Subunit composition of (R,S)-reticuline 7-O-methyltransferase = [7OMT]2
         (R,S)-reticuline 7-O-methyltransferase = 7OMT

Summary:
The enzyme is a homodimer. It methylates (S)- and (R)-reticuline with equal efficiency.

Molecular Weight of Polypeptide: 43 kD (experimental) [Ounaroon03 ]

Molecular Weight of Multimer: 85 kD (experimental) [Ounaroon03]

Relationship Links: Entrez-Nucleotide:PART-OF:AY268893

Gene-Reaction Schematic: ?

Credits:
Created 01-Feb-2007 by Zhang P , TAIR


Enzymatic reaction of: (R)-reticuline 7-O-methyltransferase ((R,S)-reticuline 7-O-methyltransferase)

EC Number: 2.1.1.291

(R)-reticuline + S-adenosyl-L-methionine <=> (R)-laudanine + S-adenosyl-L-homocysteine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

Kinetic Parameters:

Substrate
Km (μM)
Citations
(R)-reticuline
17.0
[Ounaroon03]
S-adenosyl-L-methionine
310.0
[Ounaroon03]

T(opt): 37 °C [Ounaroon03]

pH(opt): 8 [Ounaroon03]


Enzymatic reaction of: (S)-reticuline 7-O-methyltransferase ((R,S)-reticuline 7-O-methyltransferase)

EC Number: 2.1.1.291

(S)-reticuline + S-adenosyl-L-methionine <=> (S)-laudanine + S-adenosyl-L-homocysteine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: papaverine biosynthesis

Kinetic Parameters:

Substrate
Km (μM)
Citations
(S)-reticuline
16.0
[Ounaroon03]
S-adenosyl-L-methionine
360.0
[Ounaroon03]

T(opt): 37 °C [Ounaroon03]

pH(opt): 8 [Ounaroon03]


References

Ounaroon03: Ounaroon A, Decker G, Schmidt J, Lottspeich F, Kutchan TM (2003). "(R,S)-Reticuline 7-O-methyltransferase and (R,S)-norcoclaurine 6-O-methyltransferase of Papaver somniferum - cDNA cloning and characterization of methyl transfer enzymes of alkaloid biosynthesis in opium poppy." Plant J 36(6);808-19. PMID: 14675446


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc14.