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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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for maintenance.
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MetaCyc Enzyme: (R,S)-reticuline 7-O-methyltransferase

Gene: 7OMT Accession Number: G-9902 (MetaCyc)

Synonyms: PSOMT1

Species: Papaver somniferum

Subunit composition of (R,S)-reticuline 7-O-methyltransferase = [7OMT]2
         (R,S)-reticuline 7-O-methyltransferase = 7OMT

Summary:
The enzyme is a homodimer. It methylates (S)- and (R)-reticuline with equal efficiency.

Molecular Weight of Polypeptide: 43 kD (experimental) [Ounaroon03 ]

Molecular Weight of Multimer: 85 kD (experimental) [Ounaroon03]

Relationship Links: Entrez-Nucleotide:PART-OF:AY268893

Gene-Reaction Schematic: ?

Credits:
Created 01-Feb-2007 by Zhang P , TAIR


Enzymatic reaction of: (R)-reticuline 7-O-methyltransferase ((R,S)-reticuline 7-O-methyltransferase)

EC Number: 2.1.1.291

(R)-reticuline + S-adenosyl-L-methionine <=> (R)-laudanine + S-adenosyl-L-homocysteine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

Kinetic Parameters:

Substrate
Km (μM)
Citations
(R)-reticuline
17.0
[Ounaroon03]
S-adenosyl-L-methionine
310.0
[Ounaroon03]

T(opt): 37 °C [Ounaroon03]

pH(opt): 8 [Ounaroon03]


Enzymatic reaction of: (S)-reticuline 7-O-methyltransferase ((R,S)-reticuline 7-O-methyltransferase)

EC Number: 2.1.1.291

(S)-reticuline + S-adenosyl-L-methionine <=> (S)-laudanine + S-adenosyl-L-homocysteine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: papaverine biosynthesis

Kinetic Parameters:

Substrate
Km (μM)
Citations
(S)-reticuline
16.0
[Ounaroon03]
S-adenosyl-L-methionine
360.0
[Ounaroon03]

T(opt): 37 °C [Ounaroon03]

pH(opt): 8 [Ounaroon03]


References

Ounaroon03: Ounaroon A, Decker G, Schmidt J, Lottspeich F, Kutchan TM (2003). "(R,S)-Reticuline 7-O-methyltransferase and (R,S)-norcoclaurine 6-O-methyltransferase of Papaver somniferum - cDNA cloning and characterization of methyl transfer enzymes of alkaloid biosynthesis in opium poppy." Plant J 36(6);808-19. PMID: 14675446


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc13.