Species: Aromatoleum aromaticum EbN1
The enzymes involved in the 1-ethyl-4-hydroxybenzene degradation pathway of Aromatoleum aromaticum EbN1 are encoded in a single large operon ( approximately 15 kb). They include a p-cresol methylhydroxylase-like protein (encoded by pchC and pchF), proposed to be a p-ethylphenol methylhydroxylase, two predicted alcohol dehydrogenases (encoded by chnA and ebA309), a biotin-dependent carboxylase (encoded by xccA, xccB and xccC), an acetoacetyl-CoA synthetase-like protein (acsA) and a thiolase (tioL).
None of these enzymes has been verified biochemically yet. However, expression of these proteins was shown to be upregulated in cells adapted to anaerobic growth with 1-ethyl-4-hydroxybenzene and 4-hydroxyacetophenone [Wohlbrand08].
Enzymatic reaction of: p-ethylphenol methylhydroxylase
EC Number: 1.17.99.-
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is favored in the direction shown.
In Pathways: 4-ethylphenol degradation (anaerobic)
|Gene:||pchC||Accession Number: G-10958 (MetaCyc)|
Molecular Weight: 12.371 kD (from nucleotide sequence)
|Gene:||pchF||Accession Number: G-10959 (MetaCyc)|
Molecular Weight: 61.737 kD (from nucleotide sequence)
Relationship Links: InterPro:IN-FAMILY:IPR004113 , InterPro:IN-FAMILY:IPR006094 , InterPro:IN-FAMILY:IPR016164 , InterPro:IN-FAMILY:IPR016166 , InterPro:IN-FAMILY:IPR016167 , InterPro:IN-FAMILY:IPR016169 , InterPro:IN-FAMILY:IPR016170 , InterPro:IN-FAMILY:IPR016171 , Pfam:IN-FAMILY:PF01565 , Pfam:IN-FAMILY:PF02913 , Prosite:IN-FAMILY:PS51387
Wohlbrand08: Wohlbrand L, Wilkes H, Halder T, Rabus R (2008). "Anaerobic degradation of p-ethylphenol by "Aromatoleum aromaticum" strain EbN1: pathway, regulation, and involved proteins." J Bacteriol 190(16);5699-709. PMID: 18539747
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