Species: Aromatoleum aromaticum EbN1
The enzymes involved in the 1-ethyl-4-hydroxybenzene degradation pathway of Aromatoleum aromaticum EbN1 are encoded in a single large operon ( approximately 15 kb). They include a p-cresol methylhydroxylase-like protein (encoded by pchC and pchF), proposed to be a p-ethylphenol methylhydroxylase, two predicted alcohol dehydrogenases (encoded by chnA and ebA309), a biotin-dependent carboxylase (encoded by xccA, xccB and xccC), an acetoacetyl-CoA synthetase-like protein ( acsA) and a thiolase ( tioL).
None of these enzymes has been verified biochemically yet. However, expression of these proteins was shown to be upregulated in cells adapted to anaerobic growth with 1-ethyl-4-hydroxybenzene and 4-hydroxyacetophenone [Wohlbrand08].
Enzymatic reaction of: p-ethylphenol methylhydroxylase
EC Number: 1.17.99.-1-ethyl-4-hydroxybenzene + an oxidized unknown electron acceptor + H2O → S-1-(4-hydroxyphenyl)-ethanol + an reduced unknown electron acceptor
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.
The reaction is favored in the direction shown.
In Pathways: 4-ethylphenol degradation (anaerobic)
|Gene:||pchC||Accession Number: G-10958 (MetaCyc)|
Molecular Weight: 12.371 kD (from nucleotide sequence)
|Gene:||pchF||Accession Number: G-10959 (MetaCyc)|
Molecular Weight: 61.737 kD (from nucleotide sequence)
Relationship Links: InterPro:IN-FAMILY:IPR004113, InterPro:IN-FAMILY:IPR006094, InterPro:IN-FAMILY:IPR016164, InterPro:IN-FAMILY:IPR016166, InterPro:IN-FAMILY:IPR016167, InterPro:IN-FAMILY:IPR016169, InterPro:IN-FAMILY:IPR016170, InterPro:IN-FAMILY:IPR016171, Pfam:IN-FAMILY:PF01565, Pfam:IN-FAMILY:PF02913, Prosite:IN-FAMILY:PS51387
Wohlbrand08: Wohlbrand L, Wilkes H, Halder T, Rabus R (2008). "Anaerobic degradation of p-ethylphenol by "Aromatoleum aromaticum" strain EbN1: pathway, regulation, and involved proteins." J Bacteriol 190(16);5699-709. PMID: 18539747
©2016 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493