MetaCyc Enzyme: p-ethylphenol methylhydroxylase

Species: Aromatoleum aromaticum EbN1

Subunit composition of p-ethylphenol methylhydroxylase = [PchC][PchF]
         p-ethylphenol methylhydroxylase subunit 1 = PchC
         p-ethylphenol methylhydroxylase subunit 2 = PchF

The enzymes involved in the 1-ethyl-4-hydroxybenzene degradation pathway of Aromatoleum aromaticum EbN1 are encoded in a single large operon ( approximately 15 kb). They include a p-cresol methylhydroxylase-like protein (encoded by pchC and pchF), proposed to be a p-ethylphenol methylhydroxylase, two predicted alcohol dehydrogenases (encoded by chnA and ebA309), a biotin-dependent carboxylase (encoded by xccA, xccB and xccC), an acetoacetyl-CoA synthetase-like protein (acsA) and a thiolase (tioL).

None of these enzymes has been verified biochemically yet. However, expression of these proteins was shown to be upregulated in cells adapted to anaerobic growth with 1-ethyl-4-hydroxybenzene and 4-hydroxyacetophenone [Wohlbrand08].

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 06-Nov-2008 by Caspi R , SRI International

Enzymatic reaction of: p-ethylphenol methylhydroxylase

EC Number: 1.17.99.-

1-ethyl-4-hydroxybenzene + an oxidized unknown electron acceptor + H2O <=> S-1-(4-hydroxyphenyl)-ethanol + an reduced unknown electron acceptor

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: 4-ethylphenol degradation (anaerobic)

Subunit of p-ethylphenol methylhydroxylase: p-ethylphenol methylhydroxylase subunit 1

Synonyms: PchC

Gene: pchC Accession Number: G-10958 (MetaCyc)

Molecular Weight: 12.371 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:Q5P8T3 , String:76114.ebA299 , UniProt:Q5P8T3

Relationship Links: InterPro:IN-FAMILY:IPR009056 , Pfam:IN-FAMILY:PF13442 , Prosite:IN-FAMILY:PS51007

Subunit of p-ethylphenol methylhydroxylase: p-ethylphenol methylhydroxylase subunit 2

Synonyms: PchF

Gene: pchF Accession Number: G-10959 (MetaCyc)

Molecular Weight: 61.737 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:Q5P8T2 , String:76114.ebA300 , UniProt:Q5P8T2

Relationship Links: InterPro:IN-FAMILY:IPR004113 , InterPro:IN-FAMILY:IPR006094 , InterPro:IN-FAMILY:IPR016164 , InterPro:IN-FAMILY:IPR016166 , InterPro:IN-FAMILY:IPR016167 , InterPro:IN-FAMILY:IPR016169 , InterPro:IN-FAMILY:IPR016170 , InterPro:IN-FAMILY:IPR016171 , Pfam:IN-FAMILY:PF01565 , Pfam:IN-FAMILY:PF02913 , Prosite:IN-FAMILY:PS51387


Wohlbrand08: Wohlbrand L, Wilkes H, Halder T, Rabus R (2008). "Anaerobic degradation of p-ethylphenol by "Aromatoleum aromaticum" strain EbN1: pathway, regulation, and involved proteins." J Bacteriol 190(16);5699-709. PMID: 18539747

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sun Oct 4, 2015, BIOCYC14A.