MetaCyc Enzyme: p-ethylphenol methylhydroxylase

Species: Aromatoleum aromaticum EbN1

Subunit composition of p-ethylphenol methylhydroxylase = [PchC][PchF]
         p-ethylphenol methylhydroxylase subunit 1 = PchC
         p-ethylphenol methylhydroxylase subunit 2 = PchF

The enzymes involved in the 1-ethyl-4-hydroxybenzene degradation pathway of Aromatoleum aromaticum EbN1 are encoded in a single large operon ( approximately 15 kb). They include a p-cresol methylhydroxylase-like protein (encoded by pchC and pchF), proposed to be a p-ethylphenol methylhydroxylase, two predicted alcohol dehydrogenases (encoded by chnA and ebA309), a biotin-dependent carboxylase (encoded by xccA, xccB and xccC), an acetoacetyl-CoA synthetase-like protein ( acsA) and a thiolase ( tioL).

None of these enzymes has been verified biochemically yet. However, expression of these proteins was shown to be upregulated in cells adapted to anaerobic growth with 1-ethyl-4-hydroxybenzene and 4-hydroxyacetophenone [Wohlbrand08].

Gene-Reaction Schematic

Gene-Reaction Schematic

Created 06-Nov-2008 by Caspi R, SRI International

Enzymatic reaction of: p-ethylphenol methylhydroxylase

Inferred from experiment

EC Number: 1.17.99.-

1-ethyl-4-hydroxybenzene + an oxidized unknown electron acceptor + H2O → S-1-(4-hydroxyphenyl)-ethanol + an reduced unknown electron acceptor

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: 4-ethylphenol degradation (anaerobic)

Subunit of p-ethylphenol methylhydroxylase: p-ethylphenol methylhydroxylase subunit 1

Synonyms: PchC

Gene: pchC Accession Number: G-10958 (MetaCyc)

Molecular Weight: 12.371 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:Q5P8T3, UniProt:Q5P8T3

Relationship Links: InterPro:IN-FAMILY:IPR009056, Pfam:IN-FAMILY:PF13442, Prosite:IN-FAMILY:PS51007

Subunit of p-ethylphenol methylhydroxylase: p-ethylphenol methylhydroxylase subunit 2

Synonyms: PchF

Gene: pchF Accession Number: G-10959 (MetaCyc)

Molecular Weight: 61.737 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:Q5P8T2, UniProt:Q5P8T2

Relationship Links: InterPro:IN-FAMILY:IPR004113, InterPro:IN-FAMILY:IPR006094, InterPro:IN-FAMILY:IPR016164, InterPro:IN-FAMILY:IPR016166, InterPro:IN-FAMILY:IPR016167, InterPro:IN-FAMILY:IPR016169, InterPro:IN-FAMILY:IPR016170, InterPro:IN-FAMILY:IPR016171, Pfam:IN-FAMILY:PF01565, Pfam:IN-FAMILY:PF02913, Prosite:IN-FAMILY:PS51387


Wohlbrand08: Wohlbrand L, Wilkes H, Halder T, Rabus R (2008). "Anaerobic degradation of p-ethylphenol by "Aromatoleum aromaticum" strain EbN1: pathway, regulation, and involved proteins." J Bacteriol 190(16);5699-709. PMID: 18539747

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by Pathway Tools version 19.5 (software by SRI International) on Wed Nov 25, 2015, biocyc12.