|Gene:||luxS||Accession Number: G-11132 (MetaCyc)|
Species: Vibrio harveyi
Subunit composition of
S-ribosylhomocysteine lyase = [LuxS]2
S-ribosylhomocysteine lyase monomer = LuxS
S-ribosylhomocysteine lyase (LuxS) is a small metalloenzyme with Fe2+ ion at its active site [Zhu03]. LuxS was shown to exist as a homodimer in its biologically active form, with two identical active sites being formed at the dimer interface [Lewis01a]. LuxS is unique in its ability to catalyze a non-redox cleavage of a stable thioether bond without the need for any redox-active cofactor [Pei04].
The luxS gene of Vibrio harveyi was cloned by its ability to confer AI-2 production on Escherichia coli K-12 strain DH5α, which contains a defective version of the gene [Surette99]. Inactivation of the gene in the Vibrio harveyi chromosome eliminated the ability to produce AI-2 [Surette99].
Molecular Weight of Polypeptide: 15.569 kD (from nucleotide sequence)
Unification Links: UniProt:B0LUE4
Enzymatic reaction of: S-ribosylhomocysteine lyase
EC Number: 184.108.40.206
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: autoinducer AI-2 biosynthesis II (Vibrio)
Cofactors or Prosthetic Groups: Fe2+
Lewis01a: Lewis HA, Furlong EB, Laubert B, Eroshkina GA, Batiyenko Y, Adams JM, Bergseid MG, Marsh CD, Peat TS, Sanderson WE, Sauder JM, Buchanan SG (2001). "A structural genomics approach to the study of quorum sensing: crystal structures of three LuxS orthologs." Structure 9(6);527-37. PMID: 11435117
Surette99: Surette MG, Miller MB, Bassler BL (1999). "Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production." Proc Natl Acad Sci U S A 96(4);1639-44. PMID: 9990077
©2015 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493