Pathway Tools
Intro Tutorial
discounted registration ends Sept 5, 2015
Pathway Tools
Intro Tutorial
discounted registration ends Sept 5, 2015
Pathway Tools
Intro Tutorial
discounted registration ends Sept 5, 2015
Pathway Tools
Intro Tutorial
discounted registration ends Sept 5, 2015
Pathway Tools
Intro Tutorial
discounted registration ends Sept 5, 2015

MetaCyc Enzyme: carbonic anhydrase 2

Gene: yadF Accession Number: EG12319 (MetaCyc)

Synonyms: b0126, can

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of carbonic anhydrase 2 = [YadF]4
         carbonic anhydrase 2 monomer = YadF

E. coli encodes two carbonic anhydrases, Can (carbonic anhydrase 2) and CynT (carbonic anhydrase 1). Can belongs to Clade B of the β class of carbonic anhydrases [Smith00a]. Carbonic anhydrase 2 is essential for growth at amospheric pCO2 [Hashimoto03, Merlin03, Baba06]. Enzymatic activity of carbonic anhydrase 2 is pH-dependent [Cronk01].

Crystal structures of carbonic anhydrase 2 have been solved. The crystal structure data as well as size exclusion chromatography indicate that the protein exists as a homotetramer in solution [Cronk01]. There is structural evidence for a noncatalytic binding site for bicarbonate [Cronk06].

A can mutant does not grow under normal atmospheric conditions, but the mutant can grow under conditions with a high exogenous or endogenous supply of CO2. Expression of cynT suppresses phenotypes of a can mutant [Merlin03]. A can cynT double mutant is only viable under high CO2 conditions [Hashimoto03].

Expression of can is inversely proportional to the growth rate and does not depend on CO2 levels [Merlin03]. Under anaerobiosis, FNR represses can gene expression, but it is not known if this regulation is direct or indirect [Salmon03].

Can: "carbonic anhydrase" [Merlin03]

Reviews: [Smith00a, Kozliak00, Tripp01]

Citations: [BolanosGarcia06]

Locations: cytosol

Map Position: [142,008 <- 142,670]

Molecular Weight of Polypeptide: 25.097 kD (from nucleotide sequence)

Unification Links: DIP:DIP-36168N , EcoGene:EG12319 , EcoliWiki:b0126 , ModBase:P61517 , OU-Microarray:b0126 , Pride:P61517 , Protein Model Portal:P61517 , RefSeq:NP_414668 , SMR:P61517 , String:511145.b0126 , UniProt:P61517

Relationship Links: InterPro:IN-FAMILY:IPR001765 , InterPro:IN-FAMILY:IPR015892 , Panther:IN-FAMILY:PTHR11002 , PDB:Structure:1I6O , PDB:Structure:1I6P , PDB:Structure:1T75 , PDB:Structure:2ESF , Pfam:IN-FAMILY:PF00484 , Prosite:IN-FAMILY:PS00704 , Prosite:IN-FAMILY:PS00705 , Smart:IN-FAMILY:SM00947

Ortholog Links: EcoO157Cyc (Escherichia coli O157:H7 str. EDL933):YADF

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred from experiment Author statement Inferred by computational analysis [UniProtGOA11a, GOA01, GOA01a, Hashimoto03, Cronk01]
GO:0015976 - carbon utilization Inferred by computational analysis [GOA01a]
Molecular Function: GO:0004089 - carbonate dehydratase activity Inferred from experiment Author statement Inferred by computational analysis [GOA01, GOA01a, Hashimoto03, Cronk01]
GO:0008270 - zinc ion binding Inferred from experiment Inferred by computational analysis [GOA01a, Cronk01, Sevcenco11]
GO:0016829 - lyase activity Inferred by computational analysis [UniProtGOA11a]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08, Lasserre06]

MultiFun Terms: metabolism central intermediary metabolism unassigned reversible reactions

Created 29-Mar-2007 by Keseler I , SRI International
Last-Curated ? 29-Mar-2007 by Keseler I , SRI International

Enzymatic reaction of: carbonic anhydrase

Synonyms: carbonate dehydratase, anhydrase, carbonate anhydrase, carbonic acid anhydrase, carboxyanhydrase, carbonate hydro-lyase

EC Number:

carbonic acid <=> CO2 + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Reversibility of this reaction is unspecified.

Sequence Features

Feature Class Location Citations Comment
Metal-Binding-Site 42
UniProt: Zinc.
Metal-Binding-Site 44
UniProt: Zinc.
Metal-Binding-Site 98
UniProt: Zinc.
Metal-Binding-Site 101
UniProt: Zinc.

10/20/97 Gene b0126 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12319; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

BolanosGarcia06: Bolanos-Garcia VM, Davies OR (2006). "Structural analysis and classification of native proteins from E. coli commonly co-purified by immobilised metal affinity chromatography." Biochim Biophys Acta 1760(9);1304-13. PMID: 16814929

Cronk01: Cronk JD, Endrizzi JA, Cronk MR, O'neill JW, Zhang KY (2001). "Crystal structure of E. coli beta-carbonic anhydrase, an enzyme with an unusual pH-dependent activity." Protein Sci 10(5);911-22. PMID: 11316870

Cronk06: Cronk JD, Rowlett RS, Zhang KY, Tu C, Endrizzi JA, Lee J, Gareiss PC, Preiss JR (2006). "Identification of a novel noncatalytic bicarbonate binding site in eubacterial beta-carbonic anhydrase." Biochemistry 45(14);4351-61. PMID: 16584170

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hashimoto03: Hashimoto M, Kato J (2003). "Indispensability of the Escherichia coli carbonic anhydrases YadF and CynT in cell proliferation at a low CO2 partial pressure." Biosci Biotechnol Biochem 67(4);919-22. PMID: 12784642

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Kozliak00: Kozliak EI, Guilloton MB, Fuchs JA, Anderson PM (2000). "Bacterial carbonic anhydrases." EXS (90);547-65. PMID: 11268536

Lasserre06: Lasserre JP, Beyne E, Pyndiah S, Lapaillerie D, Claverol S, Bonneu M (2006). "A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis." Electrophoresis 27(16);3306-21. PMID: 16858726

Merlin03: Merlin C, Masters M, McAteer S, Coulson A (2003). "Why is carbonic anhydrase essential to Escherichia coli?." J Bacteriol 185(21);6415-24. PMID: 14563877

Salmon03: Salmon K, Hung SP, Mekjian K, Baldi P, Hatfield GW, Gunsalus RP (2003). "Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR." J Biol Chem 278(32);29837-55. PMID: 12754220

Sevcenco11: Sevcenco AM, Pinkse MW, Wolterbeek HT, Verhaert PD, Hagen WR, Hagedoorn PL (2011). "Exploring the microbial metalloproteome using MIRAGE." Metallomics 3(12);1324-30. PMID: 22094925

Smith00a: Smith KS, Ferry JG (2000). "Prokaryotic carbonic anhydrases." FEMS Microbiol Rev 24(4);335-66. PMID: 10978542

Tripp01: Tripp BC, Smith K, Ferry JG (2001). "Carbonic anhydrase: new insights for an ancient enzyme." J Biol Chem 276(52);48615-8. PMID: 11696553

UniProt15: UniProt Consortium (2015). "UniProt version 2015-01 released on 2015-01-16 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue Sep 1, 2015, biocyc14.