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discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: phosphate acetyltransferase

Gene: eutD Accession Numbers: G7288 (MetaCyc), b2458, ECK2453

Synonyms: ypfA, eutI

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of phosphate acetyltransferase = [EutD]2
         phosphate acetyltransferase monomer = EutD

Summary:
The EutD protein has phosphotransacetylase activity. Expression of EutD - either from a plasmid or by inducing expression of the eut operon with ethanolamine and vitamin B12 - can rescue the growth defect of a pta acs mutant for growth on acetate as the sole source of carbon [Bologna10].

eutD shows differential codon adaptation, resulting in differential translation efficiency signatures, in thermophilic microbes. It was therefore predicted to play a role in the heat shock response. A eutD deletion mutant was shown to be more sensitive than wild-type specifically to heat shock, but not other stresses [Krisko14].

Citations: [Wang14b]

Locations: cytosol

Map Position: [2,570,511 <- 2,571,527]

Molecular Weight of Polypeptide: 36.067 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008097 , EchoBASE:EB3940 , EcoGene:EG14188 , EcoliWiki:b2458 , ModBase:P77218 , OU-Microarray:b2458 , PortEco:eutD , PR:PRO_000022538 , Protein Model Portal:P77218 , RefSeq:NP_416953 , RegulonDB:G7288 , SMR:P77218 , String:511145.b2458 , UniProt:P77218

Relationship Links: InterPro:IN-FAMILY:IPR002505 , InterPro:IN-FAMILY:IPR004614 , InterPro:IN-FAMILY:IPR012147 , PDB:Structure:1VMI , Pfam:IN-FAMILY:PF01515

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0009408 - response to heat Inferred from experiment [Krisko14]
GO:0008152 - metabolic process Inferred by computational analysis [GOA01]
GO:0046336 - ethanolamine catabolic process Inferred by computational analysis [UniProtGOA12]
Molecular Function: GO:0008959 - phosphate acetyltransferase activity Inferred from experiment [Bologna10]
GO:0042803 - protein homodimerization activity Inferred from experiment [Bologna10]
GO:0016407 - acetyltransferase activity Inferred by computational analysis [GOA01]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
GO:0016746 - transferase activity, transferring acyl groups Inferred by computational analysis [UniProtGOA11, GOA01]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism carbon utilization

Credits:
Created in EcoCyc 01-Dec-2010 by Keseler I , SRI International
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: phosphate acetyltransferase

Synonyms: phosphotransacetylase

EC Number: 2.3.1.8

acetyl-CoA + phosphate <=> acetyl phosphate + coenzyme A

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible. [Bologna10]

In Pathways: superpathway of acetate utilization and formation , pyruvate fermentation to acetate IV , superpathway of N-acetylneuraminate degradation , superpathway of lysine degradation , superpathway of taurine degradation , acetate formation from acetyl-CoA I , mixed acid fermentation

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International

Summary:
The acetyl-phosphate forming reaction is catalyzed at nearly 4-times lower rates that the acetyl-CoA forming reaction; however, differences in Km result in similar catalytic efficiency in both directions [Bologna10].

Activators (Unknown Mechanism): pyruvate [Bologna10]

Kinetic Parameters:

Substrate
Km (μM)
Citations
acetyl-CoA
9.5
[Bologna10]
coenzyme A
32.7
[Bologna10]
acetyl phosphate
311.7
[Bologna10]

pH(opt): 7.8 [Bologna10]

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Bologna10: Bologna FP, Campos-Bermudez VA, Saavedra DD, Andreo CS, Drincovich MF (2010). "Characterization of Escherichia coli EutD: a phosphotransacetylase of the ethanolamine operon." J Microbiol 48(5);629-36. PMID: 21046341

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Krisko14: Kri Ko A, Copi T, Gabaldon T, Lehner B, Supek F (2014). "Inferring gene function from evolutionary change in signatures of translation efficiency." Genome Biol 15(3);R44. PMID: 24580753

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Wang14b: Wang JF, Meng HL, Xiong ZQ, Zhang SL, Wang Y (2014). "Identification of novel knockout and up-regulated targets for improving isoprenoid production in E. coli." Biotechnol Lett 36(5);1021-7. PMID: 24658737


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC13B.