Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
twitter

MetaCyc Enzyme: phosphate acetyltransferase

Gene: eutD Accession Numbers: G7288 (MetaCyc), b2458, ECK2453

Synonyms: ypfA, eutI

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of phosphate acetyltransferase = [EutD]2
         phosphate acetyltransferase monomer = EutD

Summary:
The EutD protein has phosphotransacetylase activity. Expression of EutD - either from a plasmid or by inducing expression of the eut operon with ethanolamine and vitamin B12 - can rescue the growth defect of a pta acs mutant for growth on acetate as the sole source of carbon [Bologna10].

eutD shows differential codon adaptation, resulting in differential translation efficiency signatures, in thermophilic microbes. It was therefore predicted to play a role in the heat shock response. A eutD deletion mutant was shown to be more sensitive than wild-type specifically to heat shock, but not other stresses [Krisko14].

Citations: [Wang14a]

Locations: cytosol

Map Position: [2,570,511 <- 2,571,527]

Molecular Weight of Polypeptide: 36.067 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008097 , EchoBASE:EB3940 , EcoGene:EG14188 , EcoliWiki:b2458 , ModBase:P77218 , OU-Microarray:b2458 , PortEco:eutD , PR:PRO_000022538 , Protein Model Portal:P77218 , RefSeq:NP_416953 , RegulonDB:G7288 , SMR:P77218 , String:511145.b2458 , UniProt:P77218

Relationship Links: InterPro:IN-FAMILY:IPR002505 , InterPro:IN-FAMILY:IPR004614 , InterPro:IN-FAMILY:IPR012147 , PDB:Structure:1VMI , Pfam:IN-FAMILY:PF01515

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0009408 - response to heat Inferred from experiment [Krisko14]
GO:0008152 - metabolic process Inferred by computational analysis [GOA01]
GO:0046336 - ethanolamine catabolic process Inferred by computational analysis [UniProtGOA12]
Molecular Function: GO:0008959 - phosphate acetyltransferase activity Inferred from experiment [Bologna10]
GO:0042803 - protein homodimerization activity Inferred from experiment [Bologna10]
GO:0016407 - acetyltransferase activity Inferred by computational analysis [GOA01]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
GO:0016746 - transferase activity, transferring acyl groups Inferred by computational analysis [UniProtGOA11, GOA01]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism carbon utilization

Credits:
Created in EcoCyc 01-Dec-2010 by Keseler I , SRI International
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: phosphate acetyltransferase

Synonyms: phosphotransacetylase

EC Number: 2.3.1.8

acetyl-CoA + phosphate <=> acetyl phosphate + coenzyme A

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible. [Bologna10]

In Pathways: superpathway of acetate utilization and formation , pyruvate fermentation to acetate IV , superpathway of N-acetylneuraminate degradation , superpathway of lysine degradation , superpathway of taurine degradation , acetate formation from acetyl-CoA I , mixed acid fermentation

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International

Summary:
The acetyl-phosphate forming reaction is catalyzed at nearly 4-times lower rates that the acetyl-CoA forming reaction; however, differences in Km result in similar catalytic efficiency in both directions [Bologna10].

Activators (Unknown Mechanism): pyruvate [Bologna10]

Kinetic Parameters:

Substrate
Km (μM)
Citations
acetyl-CoA
9.5
[Bologna10]
coenzyme A
32.7
[Bologna10]
acetyl phosphate
311.7
[Bologna10]

pH(opt): 7.8 [Bologna10]

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Bologna10: Bologna FP, Campos-Bermudez VA, Saavedra DD, Andreo CS, Drincovich MF (2010). "Characterization of Escherichia coli EutD: a phosphotransacetylase of the ethanolamine operon." J Microbiol 48(5);629-36. PMID: 21046341

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Krisko14: Kri Ko A, Copi T, Gabaldon T, Lehner B, Supek F (2014). "Inferring gene function from evolutionary change in signatures of translation efficiency." Genome Biol 15(3);R44. PMID: 24580753

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Wang14a: Wang JF, Meng HL, Xiong ZQ, Zhang SL, Wang Y (2014). "Identification of novel knockout and up-regulated targets for improving isoprenoid production in E. coli." Biotechnol Lett 36(5);1021-7. PMID: 24658737


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, BIOCYC14B.