|Gene:||eutD||Accession Numbers: G7288 (MetaCyc), b2458, ECK2453|
Synonyms: ypfA, eutI
Species: Escherichia coli K-12 substr. MG1655
Subunit composition of
phosphate acetyltransferase = [EutD]2
phosphate acetyltransferase monomer = EutD
The EutD protein has phosphotransacetylase activity. Expression of EutD - either from a plasmid or by inducing expression of the eut operon with ethanolamine and vitamin B12 - can rescue the growth defect of a pta acs mutant for growth on acetate as the sole source of carbon [Bologna10].
eutD shows differential codon adaptation, resulting in differential translation efficiency signatures, in thermophilic microbes. It was therefore predicted to play a role in the heat shock response. A eutD deletion mutant was shown to be more sensitive than wild-type specifically to heat shock, but not other stresses [Kri14].
|Map Position: [2,570,511 <- 2,571,527]|
Molecular Weight of Polypeptide: 36.067 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0008097 , EchoBASE:EB3940 , EcoGene:EG14188 , EcoliWiki:b2458 , ModBase:P77218 , OU-Microarray:b2458 , PortEco:eutD , PR:PRO_000022538 , Protein Model Portal:P77218 , RefSeq:NP_416953 , RegulonDB:G7288 , SMR:P77218 , String:511145.b2458 , UniProt:P77218
|Biological Process:||GO:0009408 - response to heat
GO:0008152 - metabolic process [GOA01]
GO:0046336 - ethanolamine catabolic process [UniProtGOA12]
|Molecular Function:||GO:0008959 - phosphate acetyltransferase activity
GO:0042803 - protein homodimerization activity [Bologna10]
GO:0016407 - acetyltransferase activity [GOA01]
GO:0016740 - transferase activity [UniProtGOA11]
GO:0016746 - transferase activity, transferring acyl groups [UniProtGOA11, GOA01]
|Cellular Component:||GO:0005829 - cytosol [DiazMejia09]|
|MultiFun Terms:||metabolism → carbon utilization|
Enzymatic reaction of: phosphate acetyltransferase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.
This reaction is reversible. [Bologna10]
In Pathways: superpathway of acetate utilization and formation , pyruvate fermentation to acetate IV , superpathway of N-acetylneuraminate degradation , superpathway of lysine degradation , superpathway of taurine degradation , acetate formation from acetyl-CoA I , mixed acid fermentation
The acetyl-phosphate forming reaction is catalyzed at nearly 4-times lower rates that the acetyl-CoA forming reaction; however, differences in Km result in similar catalytic efficiency in both directions [Bologna10].
pH(opt): 7.8 [Bologna10]
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.
Bologna10: Bologna FP, Campos-Bermudez VA, Saavedra DD, Andreo CS, Drincovich MF (2010). "Characterization of Escherichia coli EutD: a phosphotransacetylase of the ethanolamine operon." J Microbiol 48(5);629-36. PMID: 21046341
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Wang14: Wang JF, Meng HL, Xiong ZQ, Zhang SL, Wang Y (2014). "Identification of novel knockout and up-regulated targets for improving isoprenoid production in E. coli." Biotechnol Lett 36(5);1021-7. PMID: 24658737
©2014 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493