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MetaCyc Polypeptide: 30S ribosomal subunit protein S8

Gene: rpsH Accession Numbers: EG10907 (MetaCyc), b3306, ECK3293

Species: Escherichia coli K-12 substr. MG1655

Component of:
30S ribosomal subunit
ribosome

Summary:
The S8 protein is a component of the 30S subunit of the ribosome and also functions in the post-transcriptional regulation of the ribosomal protein genes encoded in the spc operon.

The S8 protein binds to 16S rRNA [Schaup73] in the absence of other ribosomal proteins [Schaup70]. S8 binding is not absolutely required for assembly of the 30S ribosomal subunit platform, but it influences the organization of the 16S rRNA central domain [Jagannathan03]. Sites within 16S rRNA where S8 interacts have been determined [Wower83, Gregory84, Gregory86, Wiener88, Mougel93, Wu94], and the secondary and tertiary structure of the binding region has been investigated [Mougel87, Allmang94, Kalurachchi97, Kalurachchi98]. The unique cysteine residue present in S8 is essential for 16S rRNA binding [Mougel86], although it may not be involved in RNA recognition [Wu93a]. Other sequence determinants important for 16S rRNA binding have been investigated [Wower92, Wu93a, Moine97]. The affinity of S8 for 16S rRNA and thus the stability of the complex is correlated with the optimal growth temperature of various organisms [Gruber03].

In addition to its function in the ribosome, the ribosomal protein S8 binds to a site overlapping and sequestering the L5 initiation codon in the spc operon mRNA. It thereby represses initiation of L5 (rplE) translation [Thomas87a, Dean81, Yates80], and translational coupling ensures the coordinated regulation of genes downstream of rplE [Mattheakis88]. However, it is not known whether S8 also regulates the translation of the last two open reading frames of the spc operon, secY and rpmJ. Expression of the first two genes of the spc operon, encoding ribosomal proteins L14 and L24, is regulated by the same interaction of S8 with spc operon mRNA via "retroregulation" [Mattheakis89]. Processing of spc mRNA was shown to be stimulated by overproduction of S8 [Mattheakis88], and this effect is dependent on polynucleotide phosphorylase and RNase II [Mattheakis89].

The target site on mRNA for S8 feedback regulation shows structural similarity to the region of 16S rRNA that binds S8 [Olins81, Gregory88, Cerretti88]. The affinity of S8 for its mRNA binding site is approximately 5-fold lower than for its rRNA binding site due to differences in the secondary structure of the sites [Wu94]. A crystal structure of the complex between S8 and spc operon mRNA has been determined at 2.8 Å resolution and shows similarity to the structure of the S8/helix 21 complex in the small ribosomal subunit [Merianos04].

Locations: cytosol, ribosome

Map Position: [3,444,175 <- 3,444,567]

Molecular Weight of Polypeptide: 15.8 kD (experimental) [Tindall81 ]

Unification Links: ASAP:ABE-0010829 , CGSC:223 , DIP:DIP-47901N , EchoBASE:EB0900 , EcoGene:EG10907 , EcoliWiki:b3306 , Mint:MINT-1318565 , ModBase:P0A7W7 , OU-Microarray:b3306 , PortEco:b3306 , PR:PRO_000023861 , Pride:P0A7W7 , Protein Model Portal:P0A7W7 , RefSeq:NP_417765 , RegulonDB:EG10907 , SMR:P0A7W7 , String:511145.b3306 , UniProt:P0A7W7

Relationship Links: InterPro:IN-FAMILY:IPR000630 , Panther:IN-FAMILY:PTHR11758 , PDB:Structure:1EG0 , PDB:Structure:1M5G , PDB:Structure:1P6G , PDB:Structure:1P87 , PDB:Structure:1S03 , PDB:Structure:1VS5 , PDB:Structure:1VS7 , PDB:Structure:2AVY , PDB:Structure:2AW7 , PDB:Structure:2GY9 , PDB:Structure:2GYB , PDB:Structure:2I2P , PDB:Structure:2I2U , PDB:Structure:2QAL , PDB:Structure:2QAN , PDB:Structure:2QB9 , PDB:Structure:2QBB , PDB:Structure:2QBD , PDB:Structure:2QBF , PDB:Structure:2QBH , PDB:Structure:2QBJ , PDB:Structure:2QOU , PDB:Structure:2QOW , PDB:Structure:2QOY , PDB:Structure:2QP0 , PDB:Structure:2VHO , PDB:Structure:2VHP , PDB:Structure:2WWL , PDB:Structure:2YKR , PDB:Structure:2Z4K , PDB:Structure:2Z4M , PDB:Structure:3DF1 , PDB:Structure:3DF3 , PDB:Structure:3E1A , PDB:Structure:3E1C , PDB:Structure:3FIH , PDB:Structure:3I1M , PDB:Structure:3I1O , PDB:Structure:3I1Q , PDB:Structure:3I1S , PDB:Structure:3I1Z , PDB:Structure:3I21 , PDB:Structure:3IZV , PDB:Structure:3IZW , PDB:Structure:3J00 , PDB:Structure:3J0U , PDB:Structure:3J0V , PDB:Structure:3J0X , PDB:Structure:3J0Z , PDB:Structure:3J10 , PDB:Structure:3J13 , PDB:Structure:3J18 , PDB:Structure:3J36 , PDB:Structure:3KC4 , PDB:Structure:3OAQ , PDB:Structure:3OAR , PDB:Structure:3OFA , PDB:Structure:3OFB , PDB:Structure:3OFO , PDB:Structure:3OFP , PDB:Structure:3OFX , PDB:Structure:3OFY , PDB:Structure:3OR9 , PDB:Structure:3ORA , PDB:Structure:3SFS , PDB:Structure:3UOQ , PDB:Structure:4A2I , PDB:Structure:4ADV , PDB:Structure:4GAQ , PDB:Structure:4GAS , PDB:Structure:4GD1 , PDB:Structure:4GD2 , PDB:Structure:4KIY , PDB:Structure:4KJ0 , PDB:Structure:4KJ2 , PDB:Structure:4KJ4 , PDB:Structure:4KJ6 , PDB:Structure:4KJ8 , PDB:Structure:4KJA , PDB:Structure:4KJC , Pfam:IN-FAMILY:PF00410 , Prosite:IN-FAMILY:PS00053

Ortholog Links: Ecol199310Cyc (Escherichia coli CFT073):C4070 , EcoO157Cyc (Escherichia coli O157:H7 str. EDL933):RPSH , ShigellaCyc (Shigella flexneri 2a str. 2457T):RPSH

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0043488 - regulation of mRNA stability Inferred from experiment [Mattheakis89]
GO:0006412 - translation Inferred by computational analysis [GOA01a]
GO:0006417 - regulation of translation Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0019843 - rRNA binding Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Mizushima70]
GO:0003723 - RNA binding Inferred by computational analysis [UniProtGOA11a]
GO:0003735 - structural constituent of ribosome Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08]
GO:0022627 - cytosolic small ribosomal subunit Inferred from experiment [Hindennach71, WittmannLiebold73]
GO:0005840 - ribosome Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0030529 - ribonucleoprotein complex Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell structure ribosomes
information transfer protein related ribosomal proteins
information transfer protein related translation
regulation type of regulation posttranscriptional

Credits:
Last-Curated ? 14-Mar-2006 by Keseler I , SRI International


Subunit of: 30S ribosomal subunit

Synonyms: ribosome, small subunit

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of 30S ribosomal subunit = [RpsA][RpsB][RpsC][RpsD][RpsE][RpsF][RpsG][RpsH][RpsI][RpsJ][RpsK][RpsL][RpsM][RpsN][RpsO][RpsP][RpsQ][RpsR][RpsS][RpsT][RpsU][Sra][16S ribosomal RNA (rrsA)]
         30S ribosomal subunit protein S1 = RpsA (extended summary available)
         30S ribosomal subunit protein S2 = RpsB (summary available)
         30S ribosomal subunit protein S3 = RpsC (summary available)
         30S ribosomal subunit protein S4 = RpsD (extended summary available)
         30S ribosomal subunit protein S5 = RpsE (extended summary available)
         30S ribosomal subunit protein S6 = RpsF (extended summary available)
         30S ribosomal subunit protein S7 = RpsG (extended summary available)
         30S ribosomal subunit protein S8 = RpsH (extended summary available)
         30S ribosomal subunit protein S9 = RpsI (extended summary available)
         30S ribosomal subunit protein S10 = RpsJ (extended summary available)
         30S ribosomal subunit protein S11 = RpsK (summary available)
         30S ribosomal subunit protein S12 = RpsL (extended summary available)
         30S ribosomal subunit protein S13 = RpsM (extended summary available)
         30S ribosomal subunit protein S14 = RpsN (summary available)
         30S ribosomal subunit protein S15 = RpsO (extended summary available)
         30S ribosomal subunit protein S16 = RpsP (summary available)
         30S ribosomal subunit protein S17 = RpsQ (summary available)
         30S ribosomal subunit protein S18 = RpsR (extended summary available)
         30S ribosomal subunit protein S19 = RpsS (summary available)
         30S ribosomal subunit protein S20 = RpsT (extended summary available)
         30S ribosomal subunit protein S21 = RpsU (summary available)
         30S ribosomal subunit protein S22 = Sra (summary available)

Component of: ribosome

Enzymes activated by 30S ribosomal subunit, sorted by the type of activation, are:

Activator (Mechanism unknown) of: GTPase [Daigle04, Himeno04]

Credits:
Created 28-Mar-2006 by Keseler I , SRI International


Subunit of: ribosome

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of ribosome = [(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)(16S ribosomal RNA (rrsA))][(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplJ)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)(5S ribosomal RNA (rrfA))(23S ribosomal RNA (rrlA))]
         30S ribosomal subunit = (RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)(16S ribosomal RNA (rrsA))
                 30S ribosomal subunit protein S1 = RpsA (extended summary available)
                 30S ribosomal subunit protein S2 = RpsB (summary available)
                 30S ribosomal subunit protein S3 = RpsC (summary available)
                 30S ribosomal subunit protein S4 = RpsD (extended summary available)
                 30S ribosomal subunit protein S5 = RpsE (extended summary available)
                 30S ribosomal subunit protein S6 = RpsF (extended summary available)
                 30S ribosomal subunit protein S7 = RpsG (extended summary available)
                 30S ribosomal subunit protein S8 = RpsH (extended summary available)
                 30S ribosomal subunit protein S9 = RpsI (extended summary available)
                 30S ribosomal subunit protein S10 = RpsJ (extended summary available)
                 30S ribosomal subunit protein S11 = RpsK (summary available)
                 30S ribosomal subunit protein S12 = RpsL (extended summary available)
                 30S ribosomal subunit protein S13 = RpsM (extended summary available)
                 30S ribosomal subunit protein S14 = RpsN (summary available)
                 30S ribosomal subunit protein S15 = RpsO (extended summary available)
                 30S ribosomal subunit protein S16 = RpsP (summary available)
                 30S ribosomal subunit protein S17 = RpsQ (summary available)
                 30S ribosomal subunit protein S18 = RpsR (extended summary available)
                 30S ribosomal subunit protein S19 = RpsS (summary available)
                 30S ribosomal subunit protein S20 = RpsT (extended summary available)
                 30S ribosomal subunit protein S21 = RpsU (summary available)
                 30S ribosomal subunit protein S22 = Sra (summary available)
         50S ribosomal subunit = (RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplJ)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)(5S ribosomal RNA (rrfA))(23S ribosomal RNA (rrlA))
                 50S ribosomal subunit protein L1 = RplA (extended summary available)
                 50S ribosomal subunit protein L2 = RplB (summary available)
                 50S ribosomal subunit protein L3 = RplC (summary available)
                 50S ribosomal subunit protein L4 = RplD (extended summary available)
                 50S ribosomal subunit protein L5 = RplE (summary available)
                 50S ribosomal subunit protein L6 = RplF (summary available)
                 50S ribosomal protein complex L8 = (RplJ)([RplL]2)2
                         50S ribosomal subunit protein L10 = RplJ (extended summary available)
                         50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                                 50S ribosomal subunit protein L12 = RplL
                 50S ribosomal subunit protein L9 = RplI (summary available)
                 50S ribosomal subunit protein L10 = RplJ (extended summary available)
                 50S ribosomal subunit protein L11 = RplK (extended summary available)
                 50S ribosomal subunit protein L13 = RplM (extended summary available)
                 50S ribosomal subunit protein L14 = RplN (extended summary available)
                 50S ribosomal subunit protein L15 = RplO (summary available)
                 50S ribosomal subunit protein L16 = RplP (extended summary available)
                 50S ribosomal subunit protein L17 = RplQ (summary available)
                 50S ribosomal subunit protein L18 = RplR (extended summary available)
                 50S ribosomal subunit protein L19 = RplS (extended summary available)
                 50S ribosomal subunit protein L20 = RplT (extended summary available)
                 50S ribosomal subunit protein L21 = RplU (summary available)
                 50S ribosomal subunit protein L22 = RplV (extended summary available)
                 50S ribosomal subunit protein L23 = RplW (extended summary available)
                 50S ribosomal subunit protein L24 = RplX (summary available)
                 50S ribosomal subunit protein L25 = RplY (summary available)
                 50S ribosomal subunit protein L27 = RpmA (extended summary available)
                 50S ribosomal subunit protein L28 = RpmB (summary available)
                 50S ribosomal subunit protein L29 = RpmC (summary available)
                 50S ribosomal subunit protein L30 = RpmD (summary available)
                 50S ribosomal subunit protein L31 = RpmE (summary available)
                 50S ribosomal subunit protein L32 = RpmF (summary available)
                 50S ribosomal subunit protein L33 = RpmG (summary available)
                 50S ribosomal subunit protein L34 = RpmH (summary available)
                 50S ribosomal subunit protein L35 = RpmI (summary available)
                 50S ribosomal subunit protein L36 = RpmJ (summary available)

Credits:
Created 15-Jun-2006 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Cleavage-of-Initial-Methionine 1
[Allen78, UniProt11a]
UniProt: Removed.
Chain 2 -> 130
[UniProt09]
UniProt: 30S ribosomal protein S8;
Sequence-Conflict 91
[Cerretti83, UniProt10a]
Alternate sequence: Q; UniProt: (in Ref. 1; CAA25719);

History:
10/20/97 Gene b3306 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10907; confirmed by SwissProt match.


References

Allen78: Allen G, Wittmann-Liebold B (1978). "The amino acid sequence of the ribosomal protein S8 of Escherichia coli." Hoppe Seylers Z Physiol Chem 359(11);1509-25. PMID: 365698

Allmang94: Allmang C, Mougel M, Westhof E, Ehresmann B, Ehresmann C (1994). "Role of conserved nucleotides in building the 16S rRNA binding site of E. coli ribosomal protein S8." Nucleic Acids Res 22(18);3708-14. PMID: 7937081

Cerretti83: Cerretti DP, Dean D, Davis GR, Bedwell DM, Nomura M (1983). "The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene." Nucleic Acids Res 11(9);2599-616. PMID: 6222285

Cerretti88: Cerretti DP, Mattheakis LC, Kearney KR, Vu L, Nomura M (1988). "Translational regulation of the spc operon in Escherichia coli. Identification and structural analysis of the target site for S8 repressor protein." J Mol Biol 204(2);309-29. PMID: 2464692

Daigle04: Daigle DM, Brown ED (2004). "Studies of the interaction of Escherichia coli YjeQ with the ribosome in vitro." J Bacteriol 186(5);1381-7. PMID: 14973029

Dean81: Dean D, Yates JL, Nomura M (1981). "Escherichia coli ribosomal protein S8 feedback regulates part of spc operon." Nature 289(5793);89-91. PMID: 6450328

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Gregory84: Gregory RJ, Zeller ML, Thurlow DL, Gourse RL, Stark MJ, Dahlberg AE, Zimmermann RA (1984). "Interaction of ribosomal proteins S6, S8, S15 and S18 with the central domain of 16 S ribosomal RNA from Escherichia coli." J Mol Biol 178(2);287-302. PMID: 6208366

Gregory86: Gregory RJ, Zimmermann RA (1986). "Site-directed mutagenesis of the binding site for ribosomal protein S8 within 16S ribosomal RNA from Escherichia coli." Nucleic Acids Res 14(14);5761-76. PMID: 3016664

Gregory88: Gregory RJ, Cahill PB, Thurlow DL, Zimmermann RA (1988). "Interaction of Escherichia coli ribosomal protein S8 with its binding sites in ribosomal RNA and messenger RNA." J Mol Biol 204(2);295-307. PMID: 2464691

Gruber03: Gruber T, Kohrer C, Lung B, Shcherbakov D, Piendl W (2003). "Affinity of ribosomal protein S8 from mesophilic and (hyper)thermophilic archaea and bacteria for 16S rRNA correlates with the growth temperatures of the organisms." FEBS Lett 549(1-3);123-8. PMID: 12914937

Himeno04: Himeno H, Hanawa-Suetsugu K, Kimura T, Takagi K, Sugiyama W, Shirata S, Mikami T, Odagiri F, Osanai Y, Watanabe D, Goto S, Kalachnyuk L, Ushida C, Muto A (2004). "A novel GTPase activated by the small subunit of ribosome." Nucleic Acids Res 32(17);5303-9. PMID: 15466596

Hindennach71: Hindennach I, Stoffler G, Wittmann HG (1971). "Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli." Eur J Biochem 23(1);7-11. PMID: 4942549

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Jagannathan03: Jagannathan I, Culver GM (2003). "Assembly of the central domain of the 30S ribosomal subunit: roles for the primary binding ribosomal proteins S15 and S8." J Mol Biol 330(2);373-83. PMID: 12823975

Kalurachchi97: Kalurachchi K, Uma K, Zimmermann RA, Nikonowicz EP (1997). "Structural features of the binding site for ribosomal protein S8 in Escherichia coli 16S rRNA defined using NMR spectroscopy." Proc Natl Acad Sci U S A 94(6);2139-44. PMID: 9122161

Kalurachchi98: Kalurachchi K, Nikonowicz EP (1998). "NMR structure determination of the binding site for ribosomal protein S8 from Escherichia coli 16 S rRNA." J Mol Biol 280(4);639-54. PMID: 9677294

Mattheakis88: Mattheakis LC, Nomura M (1988). "Feedback regulation of the spc operon in Escherichia coli: translational coupling and mRNA processing." J Bacteriol 170(10);4484-92. PMID: 3049533

Mattheakis89: Mattheakis L, Vu L, Sor F, Nomura M (1989). "Retroregulation of the synthesis of ribosomal proteins L14 and L24 by feedback repressor S8 in Escherichia coli." Proc Natl Acad Sci U S A 86(2);448-52. PMID: 2643112

Merianos04: Merianos HJ, Wang J, Moore PB (2004). "The structure of a ribosomal protein S8/spc operon mRNA complex." RNA 10(6);954-64. PMID: 15146079

Mizushima70: Mizushima S, Nomura M (1970). "Assembly mapping of 30S ribosomal proteins from E. coli." Nature 226(5252);1214. PMID: 4912319

Moine97: Moine H, Cachia C, Westhof E, Ehresmann B, Ehresmann C (1997). "The RNA binding site of S8 ribosomal protein of Escherichia coli: Selex and hydroxyl radical probing studies." RNA 3(3);255-68. PMID: 9056763

Mougel86: Mougel M, Ehresmann B, Ehresmann C (1986). "Binding of Escherichia coli ribosomal protein S8 to 16S rRNA: kinetic and thermodynamic characterization." Biochemistry 25(10);2756-65. PMID: 3521721

Mougel87: Mougel M, Eyermann F, Westhof E, Romby P, Expert-Bezancon A, Ebel JP, Ehresmann B, Ehresmann C (1987). "Binding of Escherichia coli ribosomal protein S8 to 16 S rRNA. A model for the interaction and the tertiary structure of the RNA binding site." J Mol Biol 198(1);91-107. PMID: 3323531

Mougel93: Mougel M, Allmang C, Eyermann F, Cachia C, Ehresmann B, Ehresmann C (1993). "Minimal 16S rRNA binding site and role of conserved nucleotides in Escherichia coli ribosomal protein S8 recognition." Eur J Biochem 215(3);787-92. PMID: 7689052

Olins81: Olins PO, Nomura M (1981). "Translational regulation by ribosomal protein S8 in Escherichia coli: structural homology between rRNA binding site and feedback target on mRNA." Nucleic Acids Res 9(7);1757-64. PMID: 6262737

Schaup70: Schaup HW, Green M, Kurland CG (1970). "Molecular interactions of ribosomal components. I. Identification of RNA binding sites for individual 30S ribosomal proteins." Mol Gen Genet 109(3);193-205. PMID: 4937571

Schaup73: Schaup HW, Sogin ML, Kurland CG, Woese CR (1973). "Localization of a binding site for ribosomal protein S8 within the 16S ribosomal ribonucleic acid of Escherichia coli." J Bacteriol 115(1);82-7. PMID: 4577755

Thomas87a: Thomas MS, Bedwell DM, Nomura M (1987). "Regulation of alpha operon gene expression in Escherichia coli. A novel form of translational coupling." J Mol Biol 196(2);333-45. PMID: 3309351

Tindall81: Tindall SH, Aune KC (1981). "Assessment by sedimentation equilibrium analysis of a heterologous macromolecular interaction in the presence of self-association: interaction of S5 with S8." Biochemistry 20(17);4861-6. PMID: 7028095

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt11a: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wiener88: Wiener L, Schuler D, Brimacombe R (1988). "Protein binding sites on Escherichia coli 16S ribosomal RNA; RNA regions that are protected by proteins S7, S9 and S19, and by proteins S8, S15 and S17." Nucleic Acids Res 16(4);1233-50. PMID: 3279390

WittmannLiebold73: Wittmann-Liebold B (1973). "Studies on the primary structure of 20 proteins from Escherichia coli ribosomes by means of an improved protein sequenator." FEBS Lett 36(3);247-9. PMID: 4587209

Wower83: Wower I, Brimacombe R (1983). "The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane." Nucleic Acids Res 11(5);1419-37. PMID: 6338481

Wower92: Wower I, Kowaleski MP, Sears LE, Zimmermann RA (1992). "Mutagenesis of ribosomal protein S8 from Escherichia coli: defects in regulation of the spc operon." J Bacteriol 174(4);1213-21. PMID: 1735715

Wu93a: Wu H, Wower I, Zimmermann RA (1993). "Mutagenesis of ribosomal protein S8 from Escherichia coli: expression, stability, and RNA-binding properties of S8 mutants." Biochemistry 32(18);4761-8. PMID: 8490021

Wu94: Wu H, Jiang L, Zimmermann RA (1994). "The binding site for ribosomal protein S8 in 16S rRNA and spc mRNA from Escherichia coli: minimum structural requirements and the effects of single bulged bases on S8-RNA interaction." Nucleic Acids Res 22(9);1687-95. PMID: 7515489

Yates80: Yates JL, Arfsten AE, Nomura M (1980). "In vitro expression of Escherichia coli ribosomal protein genes: autogenous inhibition of translation." Proc Natl Acad Sci U S A 77(4);1837-41. PMID: 6445562


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC13A.