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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Enzyme: predicted ring 1,2-epoxyphenylacetyl-CoA isomerase (oxepin-CoA forming)

Gene: paaG Accession Numbers: G6715 (MetaCyc), b1394, ECK1391

Synonyms: ydbT

Species: Escherichia coli K-12 substr. MG1655

Summary:
PaaG is the predicted oxepin-CoA-forming ring 1,2-epoxyphenylacetyl-CoA isomerase involved in phenylacetate catabolism. The enzyme from Pseudomonas sp. strain Y2 has been biochemically characterized [Teufel10].

A paaG mutant exhibits a defect in utilization of phenylacetate as a source of carbon [Ismail03].

PaaG: "phenylacetic acid degradation" [Ferrandez98]

Citations: [Park04]

Locations: cytosol

Map Position: [1,456,288 -> 1,457,076]

Molecular Weight of Polypeptide: 28.405 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0004661 , EchoBASE:EB3504 , EcoGene:EG13741 , EcoliWiki:b1394 , ModBase:P77467 , OU-Microarray:b1394 , PortEco:paaG , Pride:P77467 , Protein Model Portal:P77467 , RefSeq:NP_415912 , RegulonDB:G6715 , SMR:P77467 , String:511145.b1394 , UniProt:P77467

Relationship Links: InterPro:IN-FAMILY:IPR001753 , InterPro:IN-FAMILY:IPR011968 , InterPro:IN-FAMILY:IPR014748 , PDB:Structure:4FZW , Pfam:IN-FAMILY:PF00378 , Prosite:IN-FAMILY:PS00166

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0010124 - phenylacetate catabolic process Inferred from experiment Inferred by computational analysis [UniProtGOA12, GOA01, Ismail03]
GO:0008152 - metabolic process Inferred by computational analysis [GOA01]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Grishin12]
GO:0016853 - isomerase activity Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Teufel10]
GO:0042802 - identical protein binding Inferred from experiment [Grishin12]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01]
GO:0016829 - lyase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism carbon utilization carbon compounds


Enzymatic reaction of: ring 1,2-epoxyphenylacetyl-CoA isomerase (oxepin-CoA forming)

EC Number: 5.3.3.18

2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA <=> 2-oxepin-2(3H)-ylideneacetyl-CoA

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

In Pathways: superpathway of phenylethylamine degradation , phenylacetate degradation I (aerobic)

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Sequence Features

Feature Class Location Citations Comment
Extrinsic-Sequence-Variant 121
[UniProt10]
Alternate sequence: C; UniProt: (in strain: W);

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Ferrandez98: Ferrandez A, Minambres B, Garcia B, Olivera ER, Luengo JM, Garcia JL, Diaz E (1998). "Catabolism of phenylacetic acid in Escherichia coli. Characterization of a new aerobic hybrid pathway." J Biol Chem 1998;273(40);25974-86. PMID: 9748275

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Grishin12: Grishin AM, Ajamian E, Zhang L, Rouiller I, Bostina M, Cygler M (2012). "Protein-protein interactions in the β-oxidation part of the phenylacetate utilization pathway: crystal structure of the PaaF-PaaG hydratase-isomerase complex." J Biol Chem 287(45);37986-96. PMID: 22961985

Ismail03: Ismail W, El-Said Mohamed M, Wanner BL, Datsenko KA, Eisenreich W, Rohdich F, Bacher A, Fuchs G (2003). "Functional genomics by NMR spectroscopy. Phenylacetate catabolism in Escherichia coli." Eur J Biochem 270(14);3047-54. PMID: 12846838

Park04: Park SJ, Yup Lee S (2004). "New FadB homologous enzymes and their use in enhanced biosynthesis of medium-chain-length polyhydroxyalkanoates in FadB mutant Escherichia coli." Biotechnol Bioeng 86(6);681-6. PMID: 15137080

Teufel10: Teufel R, Mascaraque V, Ismail W, Voss M, Perera J, Eisenreich W, Haehnel W, Fuchs G (2010). "Bacterial phenylalanine and phenylacetate catabolic pathway revealed." Proc Natl Acad Sci U S A 107(32):14390-5. PMID: 20660314

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc14.