Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

MetaCyc Enzyme: glycerate kinase I

Gene: garK Accession Numbers: EG11175 (MetaCyc), b3124, ECK3112

Synonyms: yhaD

Species: Escherichia coli K-12 substr. MG1655

Summary:
Glycerate kinase I (GKI), encoded by garK, catalyzes the formation of 2-phosphoglycerate from D-glycerate [Hubbard98].

There are two glycerate kinases, known as GKI and GKII, in E. coli. GKI is more thermostable and has a broader pH optimum than GKII, and GKII activity is only induced by growth on glycolate [Ornston69a].

Glycerate kinase I is induced by growth on glycolate, glucarate, glycerate, and galactarate as the carbon source and is not sensitive to catabolite repression [Ornston69a, Monterrubio00].

Locations: cytosol

Map Position: [3,268,647 <- 3,269,873]

Molecular Weight of Polypeptide: 42.097 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010271 , DIP:DIP-28063N , EchoBASE:EB1162 , EcoGene:EG11175 , EcoliWiki:b3124 , ModBase:P23524 , OU-Microarray:b3124 , PortEco:garK , PR:PRO_000022751 , Pride:P23524 , Protein Model Portal:P23524 , RefSeq:NP_417593 , RegulonDB:EG11175 , SMR:P23524 , String:511145.b3124 , UniProt:P23524

Relationship Links: InterPro:IN-FAMILY:IPR004381 , InterPro:IN-FAMILY:IPR018193 , Panther:IN-FAMILY:PTHR21599 , Pfam:IN-FAMILY:PF02595

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0042838 - D-glucarate catabolic process Inferred from experiment [Ornston69a]
GO:0046392 - galactarate catabolic process Inferred from experiment [Ornston69a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a]
GO:0031388 - organic acid phosphorylation Inferred by computational analysis [GOA01a]
Molecular Function: GO:0016301 - kinase activity Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Hubbard98]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a]
GO:0008887 - glycerate kinase activity Inferred by computational analysis [GOA01a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism carbon utilization carbon compounds

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: glycerate kinase

Synonyms: ATP:glycerate 2-phosphotransferase

EC Number: 2.7.1.165

D-glycerate + ATP <=> 2-phospho-D-glycerate + ADP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown.

In Pathways: superpathway of D-glucarate and D-galactarate degradation , superpathway of microbial D-galacturonate and D-glucuronate degradation , D-galactarate degradation I , D-glucarate degradation I , 2-O-α-mannosyl-D-glycerate degradation

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International

Kinetic Parameters:

Substrate
Km (μM)
kcat (sec-1)
kcat/Km (sec-1 μM-1)
Citations
ATP
61.0
2.5
[Hubbard98, BRENDA14]

pH(opt): 7.3-7.7 [Ornston69a]

History:
10/20/97 Gene b3124 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11175; confirmed by SwissProt match.


References

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hubbard98: Hubbard BK, Koch M, Palmer DR, Babbitt PC, Gerlt JA (1998). "Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli." Biochemistry 1998;37(41);14369-75. PMID: 9772162

Monterrubio00: Monterrubio R, Baldoma L, Obradors N, Aguilar J, Badia J (2000). "A common regulator for the operons encoding the enzymes involved in D-galactarate, D-glucarate, and D-glycerate utilization in Escherichia coli." J Bacteriol 2000;182(9);2672-4. PMID: 10762278

Ornston69a: Ornston MK, Ornston LN (1969). "Two forms of D-glycerate kinase in Escherichia coli." J Bacteriol 1969;97(3);1227-33. PMID: 4887503

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, biocyc14.