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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Enzyme: glycerate kinase I

Gene: garK Accession Numbers: EG11175 (MetaCyc), b3124, ECK3112

Synonyms: yhaD

Species: Escherichia coli K-12 substr. MG1655

Summary:
Glycerate kinase I (GKI), encoded by garK, catalyzes the formation of 2-phosphoglycerate from D-glycerate [Hubbard98].

There are two glycerate kinases, known as GKI and GKII, in E. coli. GKI is more thermostable and has a broader pH optimum than GKII, and GKII activity is only induced by growth on glycolate [Ornston69a].

Glycerate kinase I is induced by growth on glycolate, glucarate, glycerate, and galactarate as the carbon source and is not sensitive to catabolite repression [Ornston69a, Monterrubio00].

Locations: cytosol

Map Position: [3,268,647 <- 3,269,873]

Molecular Weight of Polypeptide: 42.097 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010271 , DIP:DIP-28063N , EchoBASE:EB1162 , EcoGene:EG11175 , EcoliWiki:b3124 , ModBase:P23524 , OU-Microarray:b3124 , PortEco:garK , PR:PRO_000022751 , Pride:P23524 , Protein Model Portal:P23524 , RefSeq:NP_417593 , RegulonDB:EG11175 , SMR:P23524 , String:511145.b3124 , UniProt:P23524

Relationship Links: InterPro:IN-FAMILY:IPR004381 , InterPro:IN-FAMILY:IPR018193 , Panther:IN-FAMILY:PTHR21599 , Pfam:IN-FAMILY:PF02595

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0042838 - D-glucarate catabolic process Inferred from experiment [Ornston69a]
GO:0046392 - galactarate catabolic process Inferred from experiment [Ornston69a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11]
GO:0031388 - organic acid phosphorylation Inferred by computational analysis [GOA01]
Molecular Function: GO:0016301 - kinase activity Inferred from experiment Inferred by computational analysis [UniProtGOA11, Hubbard98]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11]
GO:0008887 - glycerate kinase activity Inferred by computational analysis [GOA01]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism carbon utilization carbon compounds

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: glycerate kinase

Synonyms: ATP:glycerate 2-phosphotransferase

EC Number: 2.7.1.165

D-glycerate + ATP <=> 2-phospho-D-glycerate + ADP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown.

In Pathways: superpathway of D-glucarate and D-galactarate degradation , superpathway of microbial D-galacturonate and D-glucuronate degradation , D-galactarate degradation I , D-glucarate degradation I , 2-O-α-mannosyl-D-glycerate degradation

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International

Kinetic Parameters:

Substrate
Km (μM)
kcat (sec-1)
kcat/Km (sec-1 μM-1)
Citations
ATP
61.0
2.5
[Hubbard98, BRENDA14]

pH(opt): 7.3-7.7 [Ornston69a]

History:
10/20/97 Gene b3124 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11175; confirmed by SwissProt match.


References

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hubbard98: Hubbard BK, Koch M, Palmer DR, Babbitt PC, Gerlt JA (1998). "Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli." Biochemistry 1998;37(41);14369-75. PMID: 9772162

Monterrubio00: Monterrubio R, Baldoma L, Obradors N, Aguilar J, Badia J (2000). "A common regulator for the operons encoding the enzymes involved in D-galactarate, D-glucarate, and D-glycerate utilization in Escherichia coli." J Bacteriol 2000;182(9);2672-4. PMID: 10762278

Ornston69a: Ornston MK, Ornston LN (1969). "Two forms of D-glycerate kinase in Escherichia coli." J Bacteriol 1969;97(3);1227-33. PMID: 4887503

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc13.