|Gene:||garK||Accession Numbers: EG11175 (MetaCyc), b3124, ECK3112|
Species: Escherichia coli K-12 substr. MG1655
Glycerate kinase I (GKI), encoded by garK, catalyzes the formation of 2-phosphoglycerate from D-glycerate [Hubbard98].
There are two glycerate kinases, known as GKI and GKII, in E. coli. GKI is more thermostable and has a broader pH optimum than GKII, and GKII activity is only induced by growth on glycolate [Ornston69a].
|Map Position: [3,268,647 <- 3,269,873]|
Molecular Weight of Polypeptide: 42.097 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0010271 , DIP:DIP-28063N , EchoBASE:EB1162 , EcoGene:EG11175 , EcoliWiki:b3124 , ModBase:P23524 , OU-Microarray:b3124 , PortEco:garK , PR:PRO_000022751 , Pride:P23524 , Protein Model Portal:P23524 , RefSeq:NP_417593 , RegulonDB:EG11175 , SMR:P23524 , String:511145.b3124 , UniProt:P23524
|Biological Process:||GO:0042838 - D-glucarate catabolic process
GO:0046392 - galactarate catabolic process [Ornston69a]
GO:0016310 - phosphorylation [UniProtGOA11a]
GO:0031388 - organic acid phosphorylation [GOA01a]
|Molecular Function:||GO:0016301 - kinase activity
GO:0000166 - nucleotide binding [UniProtGOA11a]
GO:0005524 - ATP binding [UniProtGOA11a]
GO:0008887 - glycerate kinase activity [GOA01a]
GO:0016740 - transferase activity [UniProtGOA11a]
|Cellular Component:||GO:0005829 - cytosol [DiazMejia09]|
|MultiFun Terms:||metabolism → carbon utilization → carbon compounds|
Enzymatic reaction of: glycerate kinase
Synonyms: ATP:glycerate 2-phosphotransferase
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.
The reaction is physiologically favored in the direction shown.
In Pathways: superpathway of D-glucarate and D-galactarate degradation , superpathway of microbial D-galacturonate and D-glucuronate degradation , D-galactarate degradation I , D-glucarate degradation I , 2-O-α-mannosyl-D-glycerate degradation
pH(opt): 7.3-7.7 [Ornston69a]
10/20/97 Gene b3124 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11175; confirmed by SwissProt match.
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Hubbard98: Hubbard BK, Koch M, Palmer DR, Babbitt PC, Gerlt JA (1998). "Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli." Biochemistry 1998;37(41);14369-75. PMID: 9772162
Monterrubio00: Monterrubio R, Baldoma L, Obradors N, Aguilar J, Badia J (2000). "A common regulator for the operons encoding the enzymes involved in D-galactarate, D-glucarate, and D-glycerate utilization in Escherichia coli." J Bacteriol 2000;182(9);2672-4. PMID: 10762278
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