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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Enzyme: glycerate kinase II

Gene: glxK Accession Numbers: G6283 (MetaCyc), b0514, ECK0507

Synonyms: glxB5, ybbZ

Species: Escherichia coli K-12 substr. MG1655

Summary:
Glycerate kinase II (GKII), encoded by glxK, catalyzes the formation of 3-phosphoglycerate from D-glycerate [Doughty66].

There are two glycerate kinases, known as GKI and GKII, in E. coli. GKII is less thermostable and has a narrower pH optimum than GKI [Ornston69a].

Glycerate kinase II is induced by growth on glycolate as the carbon source [Ornston69a, Doughty66].

A glxK mutant is unable to utilize glyoxylate [Cusa99].

Locations: cytosol

Map Position: [541,112 -> 542,257]

Molecular Weight of Polypeptide: 38.734 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001773 , EchoBASE:EB3386 , EcoGene:EG13621 , EcoliWiki:b0514 , ModBase:P77364 , OU-Microarray:b0514 , PortEco:glxK , PR:PRO_000022815 , Pride:P77364 , Protein Model Portal:P77364 , RefSeq:NP_415047 , RegulonDB:G6283 , SMR:P77364 , String:511145.b0514 , UniProt:P77364

Relationship Links: InterPro:IN-FAMILY:IPR004381 , InterPro:IN-FAMILY:IPR018193 , Panther:IN-FAMILY:PTHR21599 , Pfam:IN-FAMILY:PF02595

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0009436 - glyoxylate catabolic process Inferred from experiment [Cusa99]
GO:0046296 - glycolate catabolic process Inferred from experiment Inferred by computational analysis [UniProtGOA12, Ornston69a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11]
GO:0031388 - organic acid phosphorylation Inferred by computational analysis [GOA01]
Molecular Function: GO:0008887 - glycerate kinase activity Inferred from experiment Inferred by computational analysis [GOA01a, GOA01, Doughty66]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism carbon utilization carbon compounds
metabolism central intermediary metabolism glycolate metabolism
metabolism central intermediary metabolism glyoxylate degradation

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: glycerate kinase

Synonyms: D-glycerate 3-kinase, D-glycerate kinase, ATP:glycerate 3-phosphotransferase

EC Number: 2.7.1.31

D-glycerate + ATP <=> 3-phospho-D-glycerate + ADP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown.

Alternative Substrates [Comment 1]:

In Pathways: superpathway of glycol metabolism and degradation , glycolate and glyoxylate degradation I

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International

Summary:
Purification of the enzyme and identification of 3-phosphoglycerate as the product of the reaction was done with E. coli Crook's strain [Doughty66].

Cofactors or Prosthetic Groups [Comment 2]: Mg2+ [Doughty66]

Alternative Cofactors for Mg2+: Co2+ , Mn2+ , Fe2+ , Ca2+

Inhibitors (Unknown Mechanism): EDTA [Doughty66] , p-hydroxymercuribenzoate [Doughty66] , iodoacetate [Doughty66]

Kinetic Parameters:

Substrate
Km (μM)
Citations
D-glycerate
70.0
[Ornston69a]
ATP
50.0
[Ornston69a]

pH(opt): 7 [BRENDA14, Doughty66], 7.5 [Ornston69a]

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

Cusa99: Cusa E, Obradors N, Baldoma L, Badia J, Aguilar J (1999). "Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli." J Bacteriol 1999;181(24);7479-84. PMID: 10601204

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Doughty66: Doughty CC, Hayashi JA, Guenther HL (1966). "Purification and properties of D-glycerate 3-kinase from Escherichia coli." J Biol Chem 1966;241(3);568-72. PMID: 5325263

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Ornston69a: Ornston MK, Ornston LN (1969). "Two forms of D-glycerate kinase in Escherichia coli." J Bacteriol 1969;97(3);1227-33. PMID: 4887503

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Dec 21, 2014, BIOCYC13A.