|Gene:||thiD||Accession Numbers: G7135 (MetaCyc), b2103, ECK2096|
Synonyms: thiN, thiA, thiJ, hydroxymethylpyrimidine kinase 2
Species: Escherichia coli K-12 substr. MG1655
Subunit composition of hydroxymethylpyrimidine kinase / phosphohydroxymethylpyrimidine kinase = [ThiD]4
Hydroxymethylpyrimidine (HMP) kinase is involved in the biosynthesis of thiamine phosphate. This enzyme catalyzes the conversion of hydroxymethylpyrimidine to its monophosphate (HMP-P) in the presence of ATP. Subsequently, this enzyme catalyzes the conversion of HMP-P to hydroxymethylpyrimidine pyrophosphate (HMP-PP). Therefore, HMP kinase is a bifunctional enzyme catalyzing both HMP kinase and HMP-P kinase reactions. [Mizote89, Mizote99, Reddick98]
There are two HMP kinases in E. coli, the HMP kinase described above is encoded by the thiD gene and the other HMP kinase, also referred to as pyridoxal or pyridoxamine kinase (PdxK), is encoded by the pdxK gene product. Mutations in both genes in the same cell resulted in the complete loss of HMP kinase activity [Mizote96].
Under anaerobiosis, FNR represses thiD gene expression, but it is not known if this regulation is direct or indirect [Salmon03].
|Map Position: [2,181,738 <- 2,182,538]|
Molecular Weight of Polypeptide: 28.634 kD (from nucleotide sequence), 43.0 kD (experimental) [Mizote89 ]
Unification Links: ASAP:ABE-0006958 , DIP:DIP-6867N , EchoBASE:EB3821 , EcoGene:EG14068 , EcoGene:EG20225 , EcoliWiki:b2103 , ModBase:P76422 , OU-Microarray:b2103 , PortEco:thiD , PR:PRO_000024053 , Pride:P76422 , Protein Model Portal:P76422 , RefSeq:NP_416606 , RegulonDB:G7135 , SMR:P76422 , String:511145.b2103 , Swiss-Model:P76422 , UniProt:P76422
|Biological Process:||GO:0009228 - thiamine biosynthetic process
[UniProtGOA11a, GOA01, Mizote99]
GO:0009229 - thiamine diphosphate biosynthetic process [UniProtGOA12, Mizote99]
GO:0016310 - phosphorylation [UniProtGOA11a, Mizote89]
|Molecular Function:||GO:0008902 - hydroxymethylpyrimidine kinase activity
GO:0000166 - nucleotide binding [UniProtGOA11a]
GO:0005524 - ATP binding [UniProtGOA11a]
GO:0008972 - phosphomethylpyrimidine kinase activity [GOA01a, GOA01]
GO:0016301 - kinase activity [UniProtGOA11a]
GO:0016740 - transferase activity [UniProtGOA11a]
|Cellular Component:||GO:0005829 - cytosol [DiazMejia09, Ishihama08, LopezCampistrou05]|
|MultiFun Terms:||metabolism → biosynthesis of building blocks → cofactors, small molecule carriers → thiamin|
Enzymatic reaction of: hydroxymethylpyrimidine kinase
Synonyms: ATP:4-amino-5-hydroxymethyl-2-methylpyrimidine 5-phosphotransferase, HMP kinase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is favored in the direction shown.
pH(opt): 6.0 [Mizote89]
Enzymatic reaction of: phosphohydroxymethylpyrimidine kinase
Synonyms: ATP:4-amino-2methyl-5-phosphomethylpyrimidine phosphotransferase, 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase, HMP-P kinase
EC Number: 22.214.171.124
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
3/2/1998 (pkarp) Merged genes G193/EG20225 and G7135/thiD
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532
Mizote96: Mizote T, Tsuda M, Nakazawa T, Nakayama H (1996). "The thiJ locus and its relation to phosphorylation of hydroxymethylpyrimidine in Escherichia coli." Microbiology 1996;142 ( Pt 10);2969-74. PMID: 8885414
Mizote99: Mizote T, Tsuda M, Smith DD, Nakayama H, Nakazawa T (1999). "Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase." Microbiology 1999;145 ( Pt 2);495-501. PMID: 10075431
Reddick98: Reddick JJ, Kinsland C, Nicewonger R, Christian T, Downs DM, Winkler ME, Begley TP "Overexpression, purification and characterization of two pyrimidine kinases involved in the biosynthesis of thiamin: 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase and 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase." Tetrahedron 54:15983-15991 (1998).
Salmon03: Salmon K, Hung SP, Mekjian K, Baldi P, Hatfield GW, Gunsalus RP (2003). "Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR." J Biol Chem 278(32);29837-55. PMID: 12754220
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