|Gene:||ADK||Accession Number: HS08097 (MetaCyc)|
Synonyms: adenosine 5'-phosphotransferase, AK
Species: Homo sapiens
Adenosine kinase is an abundant enzyme in mammalian tissues that catalyzes the transfer of the γ-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides.
cDNAs encoding the human enzyme has been cloned [Spychala96, McNally97]. The cDNA was expressed in Escherichia coli and produced soluble active enzyme that catalyzed the phosphorylation of adenosine with high specific activity in vitro [McNally97].
There are 2 ADK transcripts that encode a 345-amino acid form and a 362-amino acid form of the enzyme. These 2 alternately spliced forms differ only at the 5' end, where the first four encoded residues of the short form are replaced by 21 residues in the long form. When expressed, both isoforms of the enzyme phosphorylated adenosine with identical kinetics and both required Mg2+ for activity [McNally97].
|Map Position: [75,014,439 -> 75,572,175]|
Molecular Weight of Polypeptide: 40.545 kD (from nucleotide sequence)
Unification Links: ArrayExpress:P55263 , Entrez-gene:132 , Mint:MINT-5001097 , PhosphoSite:P55263 , PhylomeDB:P55263 , Pride:P55263 , Protein Model Portal:P55263 , SMR:P55263 , String:9606.ENSP00000286621 , UniProt:P55263
Relationship Links: InterPro:IN-FAMILY:IPR001805 , InterPro:IN-FAMILY:IPR002173 , InterPro:IN-FAMILY:IPR011611 , InterPro:IN-FAMILY:IPR029056 , Panther:IN-FAMILY:PTHR10584:SF24 , PDB:Structure:1BX4 , PDB:Structure:2I6A , PDB:Structure:2I6B , Pfam:IN-FAMILY:PF00294 , Prints:IN-FAMILY:PR00989 , Prosite:IN-FAMILY:PS00584
Enzymatic reaction of: adenosine kinase
Synonyms: ATP:adenosine 5'-phosphotransferase
EC Number: 126.96.36.199
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
McNally97: McNally T, Helfrich RJ, Cowart M, Dorwin SA, Meuth JL, Idler KB, Klute KA, Simmer RL, Kowaluk EA, Halbert DN (1997). "Cloning and expression of the adenosine kinase gene from rat and human tissues." Biochem Biophys Res Commun 231(3);645-50. PMID: 9070863
Spychala96: Spychala J, Datta NS, Takabayashi K, Datta M, Fox IH, Gribbin T, Mitchell BS (1996). "Cloning of human adenosine kinase cDNA: sequence similarity to microbial ribokinases and fructokinases." Proc Natl Acad Sci U S A 93(3);1232-7. PMID: 8577746
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