MetaCyc Enzyme: adenosine kinase

Gene: ADK Accession Number: HS08097 (MetaCyc)

Synonyms: adenosine 5'-phosphotransferase, AK

Species: Homo sapiens

Adenosine kinase is an abundant enzyme in mammalian tissues that catalyzes the transfer of the γ-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides.

cDNAs encoding the human enzyme has been cloned [Spychala96, McNally97]. The cDNA was expressed in Escherichia coli and produced soluble active enzyme that catalyzed the phosphorylation of adenosine with high specific activity in vitro [McNally97].

There are 2 ADK transcripts that encode a 345-amino acid form and a 362-amino acid form of the enzyme. These 2 alternately spliced forms differ only at the 5' end, where the first four encoded residues of the short form are replaced by 21 residues in the long form. When expressed, both isoforms of the enzyme phosphorylated adenosine with identical kinetics and both required Mg2+ for activity [McNally97].

Map Position: [75,014,439 -> 75,572,175]

Molecular Weight of Polypeptide: 40.545 kD (from nucleotide sequence)

Unification Links: ArrayExpress:P55263 , Entrez-gene:132 , Mint:MINT-5001097 , PhosphoSite:P55263 , PhylomeDB:P55263 , Pride:P55263 , Protein Model Portal:P55263 , SMR:P55263 , String:9606.ENSP00000286621 , UniProt:P55263

Relationship Links: InterPro:IN-FAMILY:IPR001805 , InterPro:IN-FAMILY:IPR002173 , InterPro:IN-FAMILY:IPR011611 , InterPro:IN-FAMILY:IPR029056 , Panther:IN-FAMILY:PTHR10584:SF24 , PDB:Structure:1BX4 , PDB:Structure:2I6A , PDB:Structure:2I6B , Pfam:IN-FAMILY:PF00294 , Prints:IN-FAMILY:PR00989 , Prosite:IN-FAMILY:PS00584

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 16-Sep-2010 by Caspi R , SRI International

Enzymatic reaction of: adenosine kinase

Synonyms: ATP:adenosine 5'-phosphotransferase

EC Number:

adenosine + ATP <=> AMP + ADP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of purine nucleotide salvage , adenine and adenosine salvage VI

Cofactors or Prosthetic Groups: Mg2+ [Mathews98]


Schematic showing introns, exons and/or isoforms of ADK


Mathews98: Mathews II, Erion MD, Ealick SE (1998). "Structure of human adenosine kinase at 1.5 A resolution." Biochemistry 37(45);15607-20. PMID: 9843365

McNally97: McNally T, Helfrich RJ, Cowart M, Dorwin SA, Meuth JL, Idler KB, Klute KA, Simmer RL, Kowaluk EA, Halbert DN (1997). "Cloning and expression of the adenosine kinase gene from rat and human tissues." Biochem Biophys Res Commun 231(3);645-50. PMID: 9070863

Spychala96: Spychala J, Datta NS, Takabayashi K, Datta M, Fox IH, Gribbin T, Mitchell BS (1996). "Cloning of human adenosine kinase cDNA: sequence similarity to microbial ribokinases and fructokinases." Proc Natl Acad Sci U S A 93(3);1232-7. PMID: 8577746

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sun Oct 4, 2015, biocyc13.