Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
twitter

MetaCyc Polypeptide: mannose PTS permease - ManY subunit

Gene: manY Accession Numbers: EG10568 (MetaCyc), b1818, ECK1816

Synonyms: pel, ptsM, ptsP, ptsX, II-Pman, II-Man, IICMan, Enzyme IICman

Species: Escherichia coli K-12 substr. MG1655

Component of: mannose PTS permease (extended summary available)

Summary:
ManY is a hydrophobic integral membrane protein which together with ManZ forms the transmembrane channel of the E. coli ManXYZ mannose permease [Erni87]. ManY contains a PTS Enzyme IIC domain. The ManX and ManY proteins alone are sufficient for penetration of λ phage DNA [Erni87]. The C-terminus of ManY is predicted to be on the cytoplasmic side of the membrane [Rhiel94].

Citations: [Mao95]

Locations: inner membrane

Map Position: [1,901,106 -> 1,901,906]

Molecular Weight of Polypeptide: 27.636 kD (from nucleotide sequence), 25.0 kD (experimental) [Erni87 ]

Unification Links: ASAP:ABE-0006056 , CGSC:18175 , EchoBASE:EB0563 , EcoGene:EG10568 , EcoliWiki:b1818 , OU-Microarray:b1818 , PortEco:manY , PR:PRO_000023162 , Protein Model Portal:P69801 , RefSeq:NP_416332 , RegulonDB:EG10568 , String:511145.b1818 , UniProt:P69801

Relationship Links: InterPro:IN-FAMILY:IPR004700 , InterPro:IN-FAMILY:IPR018404 , Pfam:IN-FAMILY:PF03609 , Prosite:IN-FAMILY:PS51106

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01, Erni87]
GO:0015761 - mannose transport Inferred from experiment [Erni87, Kundig71]
GO:0015764 - N-acetylglucosamine transport Inferred from experiment [JonesMortimer80]
GO:0032445 - fructose import Inferred from experiment [Ferenci74, JonesMortimer74]
GO:0061490 - glucose import into cell Inferred from experiment [Kundig71]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0022870 - protein-N(PI)-phosphohistidine-mannose phosphotransferase system transporter activity Inferred from experiment [Erni87]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred from experiment [Erni87]
GO:0016020 - membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Lasserre06]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01]

MultiFun Terms: cell structure membrane
metabolism carbon utilization carbon compounds
transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Subunit of: mannose PTS permease

Synonyms: EIIman, Enzyme II mannose

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of mannose PTS permease = [ManZ]2[ManY][ManX]2
         mannose PTS permease - ManZ subunit = ManZ (summary available)
         mannose PTS permease - ManY subunit = ManY (summary available)
         mannose PTS permease - ManX subunit = ManX (extended summary available)

Summary:
ManXYZ, the mannose PTS permease, belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates its sugar substrates in a process called group translocation. ManXYZ takes up exogenous hexoses (mannose, glucose, glucosamine, fructose, N-acetylglucosamine, etc.), releasing the phosphate esters into the cell cytoplasm in preparation for metabolism, primarily via glycolysis (reviewed in [Postma93]). The mannose transporter also transports and phosphorylates the non-metabolizable analogues 2-deoxyglucose and mannosamine [Curtis75]. The mannose transporter acts as a host factor required for the infection of E. coli by λ phage [Elliott78, Williams86]. ManX and ManY alone are sufficient for λ phage infection [Erni85, Erni87].

ManXYZ, also known as the Enzyme IIMan complex, possesses four domains in three polypeptide chains, ManX=IIABMan, ManY=IICMan and ManZ=IIDMan. They are members of the PTS Mannose-Fructose-Sorbose Family (Man Family), the "splinter group", which is not homologous to most other PTS permeases [Saier14]. The IIAB subunit (ManX) forms a homodimer that is localized to the cytoplasmic side of the membrane [Stolz93]. ManY and ManZ are integral membrane proteins with six and one transmembrane α-helical spanner(s), respectively [Huber96]. The 3-dimensional structure of IIAMan and the secondary structure of IIBMan have been determined [Nunn96, Seip97]. The subunit ratio of E.coli mannose permease is 2ManX:ManY:2ManZ [Rhiel94].

The inner membrane components of the mannose PTS permease also catalyse sugar phosphate:14C sugar transphosphorylation [Saier77] and vectorial transphosphorylation [Saier77a]. Mannose-6-phosphate and glucose-6-phosphate are effective donors for transphosphorylation of labelled mannose in washed membrane preparations in vitro. Optimal rates of transphosphorylation require low sugar and high sugar-phosphate concentrations [Saier77].| The plasmid encoded, His-tagged ManXY complex is oligomeric within the membrane and monomeric in its soluble form in the cytoplasm. Both forms exhibit PEP-dependent sugar phosphorylation and sugar phosphate-dependent transphosphorylation activities, though the kinetic properties of these reactions differ between the two forms of the protein [Aboulwafa04].

The overall PTS-mediated phosphoryl transfer reaction, requiring the two general energy coupling proteins of the PTS, Enzyme I (PtsI) and HPr (PtsH), as well as the four domains of the Enzyme IImannose complex can be represented as:

PEP → Enzyme I-Phis189 → HPr-Phis15 → Enzyme IIA-Phis10 → Enzyme IIB-Phis175-(Enzyme IICD) → hexose-6-P.

ManXYZ transports mannose with micromolar affinity.

The manXYZ operon is either constitutively expressed or inducibly expressed in response to extracellular sugar substrates depending on the E. coli strain examined. Regulation of the mannose PTS is controlled by the cyclic AMP receptor protein (CRP) - cyclic AMP complex (CRP-cAMP), the Mlc transcriptional repressor [Plumbridge98b] and the SgrS small regulatory RNA [Rice12].

Reviews: [JonesMortimer80, Erni87].

Citations: [GarciaAlles02, Gutknecht99]

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from experiment [Kundig71, Curtis75]
GO:0015761 - mannose transport Inferred from experiment [Kundig71]
GO:0015764 - N-acetylglucosamine transport Inferred from experiment [JonesMortimer80]
GO:0032445 - fructose import Inferred from experiment [Ferenci74]
GO:0061490 - glucose import into cell Inferred from experiment [Kundig71]
GO:0075733 - intracellular transport of virus Inferred from experiment [Elliott78]
Molecular Function: GO:0022855 - protein-N(PI)-phosphohistidine-glucose phosphotransferase system transporter activity Inferred from experiment [Kundig71]
GO:0022870 - protein-N(PI)-phosphohistidine-mannose phosphotransferase system transporter activity Inferred from experiment [Kundig71]
GO:0022877 - protein-N(PI)-phosphohistidine-fructose phosphotransferase system transporter activity Inferred from experiment [Ferenci74]
GO:0022880 - protein-N(PI)-phosphohistidine-N-acetylglucosamine phosphotransferase system transporter activity Inferred from experiment [JonesMortimer80]
GO:0090590 - protein-N(PI)-phosphohistidine-D-glucosamine phosphotransferase system transporter activity Inferred from experiment [JonesMortimer80, Curtis75]
GO:0090591 - protein-N(PI)-phosphohistidine-N-acetyl-mannosamine phosphotransferase system transporter activity Inferred from experiment [Plumbridge99]

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: 2-deoxy-D-glucose PTS permease (mannose PTS permease)

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: D-glucosamine PTS permease (mannose PTS permease)

Synonyms: Transport of glucosamine

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: β-D-glucose PTS permease (mannose PTS permease)

Synonyms: Transport of β-D-glucose

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International

Kinetic Parameters:

Substrate
Km (μM)
Citations
β-D-glucose
30.0
[Mao95]


Sequence Features

Feature Class Location Citations Comment
Formylation-Modification 1
[UniProt10a]
UniProt: N-formylmethionine; Non-Experimental Qualifier: probably;
Conserved-Region 1 -> 237
[UniProt09]
UniProt: PTS EIIC type-4;
Transmembrane-Region 5 -> 24
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probably;
Transmembrane-Region 50 -> 69
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probably;
Transmembrane-Region 84 -> 102
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probably;
Transmembrane-Region 106 -> 124
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probably;
Transmembrane-Region 151 -> 170
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probably;
Transmembrane-Region 182 -> 201
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probably;

History:
10/20/97 Gene b1818 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10568; confirmed by SwissProt match.


References

Aboulwafa04: Aboulwafa M, Saier MH (2004). "Characterization of soluble enzyme II complexes of the Escherichia coli phosphotransferase system." J Bacteriol 186(24);8453-62. PMID: 15576795

Curtis75: Curtis SJ, Epstein W (1975). "Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase." J Bacteriol 122(3);1189-99. PMID: 1097393

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Dietz71: Dietz GW, Heppel LA (1971). "Studies on the uptake of hexose phosphates. I. 2-Deoxyglucose and 2-deoxyglucose 6-phosphate." J Biol Chem 246(9);2881-4. PMID: 4928893

Elliott78: Elliott J, Arber W (1978). "E. coli K-12 pel mutants, which block phage lambda DNA injection, coincide with ptsM, which determines a component of a sugar transport system." Mol Gen Genet 161(1);1-8. PMID: 353494

Erni85: Erni B, Zanolari B (1985). "The mannose-permease of the bacterial phosphotransferase system. Gene cloning and purification of the enzyme IIMan/IIIMan complex of Escherichia coli." J Biol Chem 260(29);15495-503. PMID: 2999119

Erni87: Erni B, Zanolari B, Kocher HP (1987). "The mannose permease of Escherichia coli consists of three different proteins. Amino acid sequence and function in sugar transport, sugar phosphorylation, and penetration of phage lambda DNA." J Biol Chem 262(11);5238-47. PMID: 2951378

Ferenci74: Ferenci T, Kornberg HL (1974). "The role of phosphotransferase-mediated syntheses of fructose 1-phosphate and fructose 6-phosphate in the growth of Escherichia coli on fructose." Proc R Soc Lond B Biol Sci 187(1087);105-19. PMID: 4153999

GarciaAlles02: Garcia-Alles LF, Zahn A, Erni B (2002). "Sugar recognition by the glucose and mannose permeases of Escherichia coli. Steady-state kinetics and inhibition studies." Biochemistry 41(31);10077-86. PMID: 12146972

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Gutknecht99: Gutknecht R, Flukiger K, Lanz R, Erni B (1999). "Mechanism of phosphoryl transfer in the dimeric IIABMan subunit of the Escherichia coli mannose transporter." J Biol Chem 274(10);6091-6. PMID: 10037691

Huber96: Huber F, Erni B (1996). "Membrane topology of the mannose transporter of Escherichia coli K12." Eur J Biochem 239(3);810-7. PMID: 8774730

JonesMortimer74: Jones-Mortimer MC, Kornberg HL (1974). "Genetical analysis of fructose utilization by Escherichia coli." Proc R Soc Lond B Biol Sci 187(1087);121-31. PMID: 4154035

JonesMortimer80: Jones-Mortimer MC, Kornberg HL (1980). "Amino-sugar transport systems of Escherichia coli K12." J Gen Microbiol 117(2);369-76. PMID: 6252281

Kundig71: Kundig W, Roseman S (1971). "Sugar transport. II. Characterization of constitutive membrane-bound enzymes II of the Escherichia coli phosphotransferase system." J Biol Chem 246(5);1407-18. PMID: 5545083

Lasserre06: Lasserre JP, Beyne E, Pyndiah S, Lapaillerie D, Claverol S, Bonneu M (2006). "A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis." Electrophoresis 27(16);3306-21. PMID: 16858726

Mao95: Mao Q, Schunk T, Flukiger K, Erni B (1995). "Functional reconstitution of the purified mannose phosphotransferase system of Escherichia coli into phospholipid vesicles." J Biol Chem 270(10);5258-65. PMID: 7890636

Nunn96: Nunn RS, Markovic-Housley Z, Genovesio-Taverne JC, Flukiger K, Rizkallah PJ, Jansonius JN, Schirmer T, Erni B (1996). "Structure of the IIA domain of the mannose transporter from Escherichia coli at 1.7 angstroms resolution." J Mol Biol 259(3);502-11. PMID: 8676384

Plumbridge98b: Plumbridge J (1998). "Control of the expression of the manXYZ operon in Escherichia coli: Mlc is a negative regulator of the mannose PTS." Mol Microbiol 1998;27(2);369-80. PMID: 9484892

Plumbridge99: Plumbridge J, Vimr E (1999). "Convergent pathways for utilization of the amino sugars N-acetylglucosamine, N-acetylmannosamine, and N-acetylneuraminic acid by Escherichia coli." J Bacteriol 1999;181(1);47-54. PMID: 9864311

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Rhiel94: Rhiel E, Flukiger K, Wehrli C, Erni B (1994). "The mannose transporter of Escherichia coli K12: oligomeric structure, and function of two conserved cysteines." Biol Chem Hoppe Seyler 375(8);551-9. PMID: 7811395

Rice12: Rice JB, Balasubramanian D, Vanderpool CK (2012). "Small RNA binding-site multiplicity involved in translational regulation of a polycistronic mRNA." Proc Natl Acad Sci U S A 109(40);E2691-8. PMID: 22988087

Saier14: Saier MH, Reddy VS, Tamang DG, Vastermark A (2014). "The transporter classification database." Nucleic Acids Res 42(Database issue);D251-8. PMID: 24225317

Saier77: Saier MH, Feucht BU, Mora WK (1977). "Sugar phosphate: sugar transphosphorylation and exchange group translocation catalyzed by the enzyme 11 complexes of the bacterial phosphoenolpyruvate: sugar phosphotransferase system." J Biol Chem 252(24);8899-907. PMID: 336623

Saier77a: Saier MH, Cox DF, Moczydlowski EG (1977). "Sugar phosphate:sugar transphosphorylation coupled to exchange group translocation catalyzed by the enzyme II complexes of the phosphoenolpyruvate:sugar phosphotransferase system in membrane vesicles of Escherichia coli." J Biol Chem 252(24);8908-16. PMID: 336624

Seip97: Seip S, Lanz R, Gutknecht R, Flukiger K, Erni B (1997). "The fructose transporter of Bacillus subtilis encoded by the lev operon: backbone assignment and secondary structure of the IIB(Lev) subunit." Eur J Biochem 243(1-2);306-14. PMID: 9030753

Stolz93: Stolz B, Huber M, Markovic-Housley Z, Erni B (1993). "The mannose transporter of Escherichia coli. Structure and function of the IIABMan subunit." J Biol Chem 1993;268(36);27094-9. PMID: 8262947

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Williams86: Williams N, Fox DK, Shea C, Roseman S (1986). "Pel, the protein that permits lambda DNA penetration of Escherichia coli, is encoded by a gene in ptsM and is required for mannose utilization by the phosphotransferase system." Proc Natl Acad Sci U S A 83(23);8934-8. PMID: 2947241


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, BIOCYC14B.