|Gene:||Bx4||Accession Number: G-7863 (MetaCyc)|
Species: Zea mays
The maize Bx4 gene encodes a cytochrome P-450-dependent monooxygenase [Glawischnig97] with 3-hydroxyindolin-2-one monooxygenase activity (BX4). The activity of this protein was tested using microsomal fractions isolated from transgenic yeast strains expressing maize Bx4 [Frey97]. The conversion of 3-hydroxyindolin-2-one into HBOA requires an unusual ring expansion whose formation mechanism is yet unknown. To date this protein has not been purified to homogeneity.
Gene Citations: [Frey95]
Molecular Weight of Polypeptide: 59.7 kD (from nucleotide sequence)
Relationship Links: Entrez-Nucleotide:PART-OF:X81828 , InterPro:IN-FAMILY:IPR001128 , InterPro:IN-FAMILY:IPR002401 , InterPro:IN-FAMILY:IPR017972 , Pfam:IN-FAMILY:PF00067 , Prints:IN-FAMILY:PR00385 , Prints:IN-FAMILY:PR00463 , Prosite:IN-FAMILY:PS00086
Enzymatic reaction of: 3-hydroxyindolin-2-one monooxygenase
EC Number: 126.96.36.199
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is physiologically favored in the direction shown.
Frey97: Frey M, Chomet P, Glawischnig E, Stettner C, Grun S, Winklmair A, Eisenreich W, Bacher A, Meeley RB, Briggs SP, Simcox K, Gierl A (1997). "Analysis of a chemical plant defense mechanism in grasses." Science 277(5326);696-9. PMID: 9235894
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