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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Enzyme: 3-hydroxyindolin-2-one monooxygenase

Gene: Bx4 Accession Number: G-7863 (MetaCyc)

Synonyms: cyp71c1

Species: Zea mays

Summary:
The maize Bx4 gene encodes a cytochrome P-450-dependent monooxygenase [Glawischnig97] with 3-hydroxyindolin-2-one monooxygenase activity (BX4). The activity of this protein was tested using microsomal fractions isolated from transgenic yeast strains expressing maize Bx4 [Frey97]. The conversion of 3-hydroxyindolin-2-one into HBOA requires an unusual ring expansion whose formation mechanism is yet unknown. To date this protein has not been purified to homogeneity.

Gene Citations: [Frey95]

Molecular Weight of Polypeptide: 59.7 kD (from nucleotide sequence)

Unification Links: Entrez:CAA57421 , Pride:Q43250 , Protein Model Portal:Q43250 , UniProt:Q43250

Relationship Links: Entrez-Nucleotide:PART-OF:X81828 , InterPro:IN-FAMILY:IPR001128 , InterPro:IN-FAMILY:IPR002401 , InterPro:IN-FAMILY:IPR017972 , Pfam:IN-FAMILY:PF00067 , Prints:IN-FAMILY:PR00385 , Prints:IN-FAMILY:PR00463 , Prosite:IN-FAMILY:PS00086

Gene-Reaction Schematic: ?

Credits:
Revised 04-Apr-2011 by Pujar A , Boyce Thompson Institute


Enzymatic reaction of: 3-hydroxyindolin-2-one monooxygenase

EC Number: 1.14.13.139

3-hydroxyindolin-2-one + NAD(P)H + oxygen + H+ <=> HBOA + NAD(P)+ + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: superpathway of benzoxazinoid glucosides biosynthesis , DIBOA-glucoside biosynthesis


References

Frey95: Frey M, Kliem R, Saedler H, Gierl A (1995). "Expression of a cytochrome P450 gene family in maize." Mol Gen Genet 246(1);100-9. PMID: 7823905

Frey97: Frey M, Chomet P, Glawischnig E, Stettner C, Grun S, Winklmair A, Eisenreich W, Bacher A, Meeley RB, Briggs SP, Simcox K, Gierl A (1997). "Analysis of a chemical plant defense mechanism in grasses." Science 277(5326);696-9. PMID: 9235894

Glawischnig97: Glawischnig, E., Eisenreich W., Bacher A., Frey M., Gierl A. (1997). "Biosynthetic origin of oxygen atoms in DIMBOA from maize: NMR studies of 18O2." Phytochemistry 45(4):715-718.


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Dec 21, 2014, BIOCYC13B.