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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: D-sorbitol dehydrogenase

Gene: NAD-SDH Accession Number: G-9104 (MetaCyc)

Species: Malus domestica

Summary:
Following the purification procedure used to isolate the apple dimeric D-sorbitol dehydrogenase [Yamaguchi94], a second protein of lower molecular weight (42 kDa compared to 62 kDa was identified as well [Yamada98]. This 42 kDa protein was used to isolate a cDNA from a cDNA library, allowing for the characterization of the Malus domestica NAD-SDH gene. The gene was expressed in Escherichia coli and the recombinant protein was confirmed to have sorbitol dehydrogenase activity [Yamada98]. Further substrate specificity was not studied.

Although the gene was barely expressed in young apple fruits, its expression was markedly increased in the matured fruit.

Molecular Weight of Polypeptide: 42 kD (experimental) [Yamada98 ]

Unification Links: Entrez:BAA36481 , ModBase:Q9ZR22 , Protein Model Portal:Q9ZR22 , Swiss-Model:Q9ZR22 , UniProt:Q9ZR22

Relationship Links: Entrez-Nucleotide:PART-OF:AB016256 , InterPro:IN-FAMILY:IPR002085 , InterPro:IN-FAMILY:IPR002328 , InterPro:IN-FAMILY:IPR011032 , InterPro:IN-FAMILY:IPR013149 , InterPro:IN-FAMILY:IPR013154 , InterPro:IN-FAMILY:IPR016040 , Panther:IN-FAMILY:PTHR11695 , Pfam:IN-FAMILY:PF00107 , Pfam:IN-FAMILY:PF08240 , Prosite:IN-FAMILY:PS00059

Gene-Reaction Schematic: ?

Credits:
Revised 03-Nov-2010 by Caspi R , SRI International


Enzymatic reaction of: D-sorbitol dehydrogenase

D-sorbitol + NAD+ <=> keto-D-fructose + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

This reaction is reversible.

In Pathways: D-sorbitol degradation I

Kinetic Parameters:

Substrate
Km (μM)
Citations
D-sorbitol
247000.0
[Yamada98]


References

Yamada98: Yamada K, Oura Y, Mori H, Yamaki S (1998). "Cloning of NAD-dependent sorbitol dehydrogenase from apple fruit and gene expression." Plant Cell Physiol 39(12);1375-9. PMID: 10050321

Yamaguchi94: Yamaguchi H., Kanayama Y., Yamaki S. (1994). "Purification and properties of NAD-dependent sorbitol dehydrogenase from apple fruit." Plant Cell Physiol. 35(6):887-892.


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, biocyc12.