|Gene:||NAD-SDH||Accession Number: G-9104 (MetaCyc)|
Species: Malus domestica
Following the purification procedure used to isolate the apple dimeric D-sorbitol dehydrogenase [Yamaguchi94], a second protein of lower molecular weight (42 kDa compared to 62 kDa was identified as well [Yamada98a]. This 42 kDa protein was used to isolate a cDNA from a cDNA library, allowing for the characterization of the Malus domestica NAD-SDH gene. The gene was expressed in Escherichia coli and the recombinant protein was confirmed to have sorbitol dehydrogenase activity [Yamada98a]. Further substrate specificity was not studied.
Although the gene was barely expressed in young apple fruits, its expression was markedly increased in the matured fruit.
Molecular Weight of Polypeptide: 42 kD (experimental) [Yamada98a ]
Relationship Links: Entrez-Nucleotide:PART-OF:AB016256 , InterPro:IN-FAMILY:IPR002085 , InterPro:IN-FAMILY:IPR002328 , InterPro:IN-FAMILY:IPR011032 , InterPro:IN-FAMILY:IPR013149 , InterPro:IN-FAMILY:IPR013154 , InterPro:IN-FAMILY:IPR016040 , Panther:IN-FAMILY:PTHR11695 , Pfam:IN-FAMILY:PF00107 , Pfam:IN-FAMILY:PF08240 , Prosite:IN-FAMILY:PS00059
Enzymatic reaction of: D-sorbitol dehydrogenase
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
This reaction is reversible.
In Pathways: D-sorbitol degradation I
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