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MetaCyc Polypeptide: monomethylamine corrinoid protein

Gene: mtmC Accession Number: G-9439 (MetaCyc)

Species: Methanosarcina barkeri

Summary:
In vitro reconstitution of CoM methylation with methylamine was achieved with three purified proteins: a monomethylamine corrinoid protein (MMCP), the "A" isozyme of methylated [methylamine-specific corrinoid protein]:coenzyme M methyltransferase (MT2-A), and methylamine--corrinoid protein Co-methyltransferase (MMAMT). MMAMT is a 170-kDa protein with 52-kDa subunits, which is rate-limiting for methyl transfer until at a 2-fold molar excess over MMCP.

MMCP and MMAMT formed a complex detectable by size exclusion high pressure liquid chromatography [Burke97].

Molecular Weight of Polypeptide: 23.067 kD (from nucleotide sequence), 29 kD (experimental) [Burke95 ]

Unification Links: Protein Model Portal:O30641 , UniProt:O30641

Relationship Links: Entrez-Nucleotide:RELATED-TO:AF013713 , InterPro:IN-FAMILY:IPR003759 , InterPro:IN-FAMILY:IPR006158 , InterPro:IN-FAMILY:IPR012741 , PDB:Structure:3EZX , Pfam:IN-FAMILY:PF02310 , Pfam:IN-FAMILY:PF02607 , Prosite:IN-FAMILY:PS51332 , Prosite:IN-FAMILY:PS51337 , Smart:IN-FAMILY:SM01018

Reactions known to consume the compound:

methanogenesis from methylamine :
methylamine + a [Co(I) methylamine-specific corrinoid protein] → a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonium

Reactions known to produce the compound:

methanogenesis from methylamine :
a [methyl-Co(III) methylamine-specific corrinoid protein] + coenzyme M → a [Co(I) methylamine-specific corrinoid protein] + methyl-CoM

In Reactions of unknown directionality:

Not in pathways:
a [Co(II) methylamine-specific corrinoid protein] + ATP + an unknown reduced electron acceptor = a [Co(I) methylamine-specific corrinoid protein] + ADP + an unknown oxidized electron acceptor + phosphate

Credits:
Created 25-May-2006 by Caspi R , SRI International


References

Burke95: Burke SA, Krzycki JA (1995). "Involvement of the "A" isozyme of methyltransferase II and the 29-kilodalton corrinoid protein in methanogenesis from monomethylamine." J Bacteriol 177(15);4410-6. PMID: 7635826

Burke97: Burke SA, Krzycki JA (1997). "Reconstitution of Monomethylamine:Coenzyme M methyl transfer with a corrinoid protein and two methyltransferases purified from Methanosarcina barkeri." J Biol Chem 272(26);16570-7. PMID: 9195968

Burke98: Burke SA, Lo SL, Krzycki JA (1998). "Clustered genes encoding the methyltransferases of methanogenesis from monomethylamine." J Bacteriol 180(13);3432-40. PMID: 9642198


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sat Apr 25, 2015, biocyc13.