|Gene:||mtbC||Accession Number: G-9441 (MetaCyc)|
Species: Methanosarcina barkeri
Methyl transfer from dimethylamine to coenzyme M was reconstituted in vitro using three Methanosarcina barkeri proteins, which included a dimethylamine corrinoid protein MtbC, methylated [methylamine-specific corrinoid protein]:coenzyme M methyltransferase (the isoform of MT2 specific for methanogenesis from methylamines), and dimethylamine--corrinoid protein Co-methyltransferase.
dimethylamine corrinoid protein, which binds 1.0 mol of corrinoid cofactor/mol of 24-kDa polypeptide, copurified with methylated [methylamine-specific corrinoid protein]:coenzyme M methyltransferase. This complex was used to assay and purify the dimethylamine--corrinoid protein Co-methyltransferase, MtbB1. MtbB1 is a 230-kDa protein composed of 51-kDa subunits. An MtbB1:MtbC ratio of 1 was optimal for coenzyme M methylation with dimethylamine.
Purified MtbB1, MtbC, and MtbA were the sole protein requirements for in vitro dimethylamine:coenzyme M methyl transfer. MtbB1 methylated either corrinoid bound to MtbC or free cob(I)alamin with dimethylamine [Ferguson00].
Gene Citations: [Ferguson97]
Molecular Weight of Polypeptide: 22.373 kD (from nucleotide sequence), 24 kD (experimental) [Ferguson00 ]
Relationship Links: Entrez-Nucleotide:RELATED-TO:AF102623 , InterPro:IN-FAMILY:IPR003759 , InterPro:IN-FAMILY:IPR006158 , InterPro:IN-FAMILY:IPR012741 , Pfam:IN-FAMILY:PF02310 , Pfam:IN-FAMILY:PF02607 , Prosite:IN-FAMILY:PS51332 , Prosite:IN-FAMILY:PS51337 , Smart:IN-FAMILY:SM01018
Reactions known to consume the compound:
Reactions known to produce the compound:
In Reactions of unknown directionality:
Not in pathways:
a [Co(II) dimethylamine-specific corrinoid protein] + a reduced ferredoxin + ATP + H2O = a [Co(I) dimethylamine-specific corrinoid protein] + an oxidized ferredoxin + ADP + phosphate + H+
Ferguson00: Ferguson DJ, Gorlatova N, Grahame DA, Krzycki JA (2000). "Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri." J Biol Chem 275(37);29053-60. PMID: 10852929
Ferguson97: Ferguson DJ, Krzycki JA (1997). "Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri." J Bacteriol 179(3);846-52. PMID: 9006042
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