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MetaCyc Enzyme: nicotinamide riboside kinase

Gene: NRK1 Accession Number: G-10407 (MetaCyc)

Synonyms: YNL129W

Species: Saccharomyces cerevisiae

Summary:
nicotinamide riboside kinase catalyzes the synthesis of β-nicotinamide D-ribonucleotide from 1-(β-D ribofuranosyl)nicotinamide. The Saccharomyces cerevisiae NRK1 gene, which encodes the enzyme, was identified by large scale screening of purified GST-fused yeast reading frames. The gene was then expressed in Escherichia coli and purified. A null mutant was also created, confirming the proposed role of the enzyme [Bieganowski04].

Molecular Weight of Polypeptide: 27.69 kD (from nucleotide sequence)

Unification Links: DIP:DIP-8168N , Entrez-gene:855594 , Mint:MINT-4499900 , Protein Model Portal:P53915 , SGD:S000005073 , SMR:P53915 , String:4932.YNL129W , UniProt:P53915

Relationship Links: InterPro:IN-FAMILY:IPR026681 , InterPro:IN-FAMILY:IPR027417 , Panther:IN-FAMILY:PTHR23404:SF1 , Pfam:IN-FAMILY:PF00485

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Credits:
Created 03-Jan-2008 by Caspi R , SRI International


Enzymatic reaction of: nicotinate riboside kinase (nicotinamide riboside kinase)

EC Number: 2.7.1.173

β-D-ribosylnicotinate + ATP <=> β-nicotinate D-ribonucleotide + ADP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.


Enzymatic reaction of: nicotinamide riboside kinase

EC Number: 2.7.1.22

1-(β-D ribofuranosyl)nicotinamide + ATP <=> β-nicotinamide D-ribonucleotide + ADP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: NAD salvage pathway III


References

Bieganowski04: Bieganowski P, Brenner C (2004). "Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans." Cell 117(4);495-502. PMID: 15137942


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sat Apr 18, 2015, BIOCYC14B.