MetaCyc Enzyme: deacetylcolchicine N-acetyltransferase

Species: Colchicum autumnale

The microsomal fraction of immature seeds of Colchicum autumnale was obtained by high-speed centrifugation. Incubation of the microsomal fraction with 14C- (S)-autumnaline, NADPH, [C3H3]S-adenosyl-L-methionine and unlabeled acetyl-CoA yielded colchicine and demecolcine. Besides the initial steps in the biosynthetic route this reaction comprises the demethylation of demecolcine and the subsequent acetylation to colchicine. The absolute requirement for NADPH and oxygen underlines the involvement of cytochrome P-450 system(s). However, the specific membrane-bound and/or associated enzymes/enzyme complexes remain to be demonstrated [Rueffer98].

Gene-Reaction Schematic

Gene-Reaction Schematic

GO Terms:
Molecular Function:
Inferred from experimentGO:0008080 - N-acetyltransferase activity [Rueffer98]

Created 11-Mar-2008 by Pujar A, Cornell University
Revised 24-Jul-2012 by Foerster H, Boyce Thompson Institute

Enzymatic reaction of: deacetylcolchicine N-acetyltransferase

Inferred from experiment

EC Number: 2.3.1.-

deacetylcolchicine + acetyl-CoA → colchicine + coenzyme A + H+

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: colchicine biosynthesis


Rueffer98: Rueffer, M, Zenk, M.H (1998). "Microsome-mediated transformation of O-methylandrocymbine to demecolcine and colchicine." FEBS letters, 438, 111-113.

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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