|Gene:||pbsA1||Accession Number: G-10574 (MetaCyc)|
Synonyms: HO1, sll1184
Species: Synechocystis sp. PCC 6803
In animals, heme oxygenase is a part of a degradative pathway, catalyzing the rate limiting step of opening the tetrapyrrole macrocycle of protoheme, in the degradation of heme to bilirubin, a process that ensures iron recycling. The enzyme, which uses heme as both a substrate and a cofactor, produces biliverdin, iron, and carbon monoxide.
In cyanobacteria, the enzyme is involved in a biosynthetic pathway. The phytobilin chromophores of phycobiliproteins and phytochromes are biosynthesized from heme in a pathway that begins with the conversion of protoheme to biliverdin IXa.
The enzyme from Synechocystis sp. PCC 6803 is encoded by the pbsA1 gene, and was identified by its similarity to animal macrosomal heme oxidases [Cornejo98]. The gene was cloned and expressed in Escherichia coli. The coli cells expressing the gene became green colored due to the accumulation of biliverdin IXa. Activity was verified in cell extracts using mesoheme as the substrate and reduced ferredoxin [Cornejo98]. When the gene was transformed to an Synechocystis sp. PCC 6803 mutant deficient in phytochrome responses (HY1), the phenotype was restored to normal [Willows00].
Molecular Weight of Polypeptide: 27.051 kD (from nucleotide sequence), 30.0 kD (experimental) [Cornejo98 ]
Relationship Links: InterPro:IN-FAMILY:IPR002051 , InterPro:IN-FAMILY:IPR016053 , InterPro:IN-FAMILY:IPR016084 , InterPro:IN-FAMILY:IPR018207 , Panther:IN-FAMILY:PTHR10720 , PDB:Structure:1WE1 , Pfam:IN-FAMILY:PF01126 , Prints:IN-FAMILY:PR00088 , Prosite:IN-FAMILY:PS00593
Enzymatic reaction of: heme oxygenase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is physiologically favored in the direction shown.
In Pathways: phycocyanobilin biosynthesis
Cornejo98: Cornejo J, Willows RD, Beale SI (1998). "Phytobilin biosynthesis: cloning and expression of a gene encoding soluble ferredoxin-dependent heme oxygenase from Synechocystis sp. PCC 6803." Plant J 15(1);99-107. PMID: 9744099
Willows00: Willows RD, Mayer SM, Foulk MS, DeLong A, Hanson K, Chory J, Beale SI (2000). "Phytobilin biosynthesis: the Synechocystis sp. PCC 6803 heme oxygenase-encoding ho1 gene complements a phytochrome-deficient Arabidopsis thalianna hy1 mutant." Plant Mol Biol 43(1);113-20. PMID: 10949378
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