|Gene:||AtCSLC4||Accession Number: G-10686 (MetaCyc)|
Species: Arabidopsis thaliana
Heterologous expression of AtCSLC4 gene in the yeast Komagataella pastoris resulted in the production of a β-1,4 glucan. Surprisingly, only the line coexpressing AtCSLC4 and AtXT1 showed strong accumulation of β-linked glucose. However no detectable level of D-xylose was observed in the transgenic line most likly due to the non production of D-xylose in Komagataella pastoris [Cocuron07], as is the case in Saccharomyces cerevisiae [BarPeled01].
BarPeled01: Bar-Peled M, Griffith CL, Doering TL (2001). "Functional cloning and characterization of a UDP- glucuronic acid decarboxylase: the pathogenic fungus Cryptococcus neoformans elucidates UDP-xylose synthesis." Proc Natl Acad Sci U S A 98(21);12003-8. PMID: 11593010
Cocuron07: Cocuron JC, Lerouxel O, Drakakaki G, Alonso AP, Liepman AH, Keegstra K, Raikhel N, Wilkerson CG (2007). "A gene from the cellulose synthase-like C family encodes a beta-1,4 glucan synthase." Proc Natl Acad Sci U S A 104(20);8550-5. PMID: 17488821
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