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discounted EARLY registration ends Dec 31, 2014
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Enzyme: patchoulene synthase / patchoulol synthase

Gene: PatTps177 Accession Number: G-11325 (MetaCyc)

Species: Pogostemon cablin

Summary:
A cDNA clone was isolated from Pogostemon cablin. The cDNA was recombinantly expressed in E.coli and the expressed protein was purified by affinity chromatography and characterized. The protein was shown to catalyze the conversion of (2E,6E)-farnesyl diphosphate into (-)-patchoulol [Deguerry06]. The purified, multifunctional patchoulol synthase also converted (2E,6E)-farnesyl diphosphate into 13 other sesquiterpenenes but the main products (about 60%) were (-)-patchoulol, α-patchoulene, β-patchoulene, γ-patchoulene and seychellene.

Molecular Weight of Polypeptide: 64.0 kD (from nucleotide sequence), 55.0 kD (experimental) [Deguerry06 ]

Unification Links: Entrez-Nucleotide:DQ355151 , Entrez:ABC87816 , Protein Model Portal:Q49SP3 , UniProt:Q49SP3

Relationship Links: InterPro:IN-FAMILY:IPR001906 , InterPro:IN-FAMILY:IPR005630 , InterPro:IN-FAMILY:IPR008930 , InterPro:IN-FAMILY:IPR008949 , Pfam:IN-FAMILY:PF01397 , Pfam:IN-FAMILY:PF03936

Gene-Reaction Schematic: ?

Credits:
Created 27-May-2009 by Pujar A , Boyce Thompson Institute
Revised 29-Jun-2012 by Foerster H , Boyce Thompson Institute


Enzymatic reaction of: patchoulol synthase

EC Number: 4.2.3.-

(2E,6E)-farnesyl diphosphate <=> seychellene + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: patchoulol biosynthesis


Enzymatic reaction of: patchoulol synthase

EC Number: 4.2.3.-

(2E,6E)-farnesyl diphosphate <=> α-patchoulene + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: patchoulol biosynthesis


Enzymatic reaction of: patchoulene synthase

EC Number: 4.2.3.-

(2E,6E)-farnesyl diphosphate <=> γ-patchoulene + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: patchoulol biosynthesis


Enzymatic reaction of: patchoulene synthase

EC Number: 4.2.3.-

(2E,6E)-farnesyl diphosphate <=> β-patchoulene + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: patchoulol biosynthesis


Enzymatic reaction of: patchoulol synthase

EC Number: 4.2.3.70

(2E,6E)-farnesyl diphosphate + H2O <=> (-)-patchoulol + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: patchoulol biosynthesis

Cofactors or Prosthetic Groups: Mg2+ [Deguerry06], Mn2+ [Deguerry06]

Kinetic Parameters:

Substrate
Km (μM)
kcat (sec-1)
kcat/Km (sec-1 μM-1)
Citations
(2E,6E)-farnesyl diphosphate
4.45
4.3e-4
[Deguerry06]

T(opt): 40 °C [Deguerry06]

pH(opt): 7-7.5 [Deguerry06]


References

Deguerry06: Deguerry F, Pastore L, Wu S, Clark A, Chappell J, Schalk M (2006). "The diverse sesquiterpene profile of patchouli, Pogostemon cablin, is correlated with a limited number of sesquiterpene synthases." Arch Biochem Biophys 454(2);123-36. PMID: 16970904


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC14B.