|Gene:||BOT2||Accession Number: G-11372 (MetaCyc)|
Species: Botrytis cinerea
A cDNA clone was isolated from the fungus Botrytis cinerea, followed by gene inactivation and complementation by protoplast transformaion. The cDNA was recombinantly expressed in E.coli and functionally characterized. The enzyme was shown to catalyze the conversion of (2E,6E)-farnesyl diphosphate into the presilphiperfolan-8β-ol, the parent sesquiterpene of the botrydial biosynthesis pathway [Pinedo08] .
Enzymatic reaction of: presilphiperfolan-8β-ol synthase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: botrydial biosynthesis
Pinedo08: Pinedo C, Wang CM, Pradier JM, Dalmais B, Choquer M, Le Pecheur P, Morgant G, Collado IG, Cane DE, Viaud M (2008). "Sesquiterpene synthase from the botrydial biosynthetic gene cluster of the phytopathogen Botrytis cinerea." ACS Chem Biol 3(12);791-801. PMID: 19035644
©2014 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493