Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: presilphiperfolan-8β-ol synthase

Gene: BOT2 Accession Number: G-11372 (MetaCyc)

Species: Botrytis cinerea

Summary:
A cDNA clone was isolated from the fungus Botrytis cinerea, followed by gene inactivation and complementation by protoplast transformaion. The cDNA was recombinantly expressed in E.coli and functionally characterized. The enzyme was shown to catalyze the conversion of (2E,6E)-farnesyl diphosphate into the presilphiperfolan-8β-ol, the parent sesquiterpene of the botrydial biosynthesis pathway [Pinedo08] .

Gene-Reaction Schematic: ?

Credits:
Created 22-Jun-2009 by Pujar A , Boyce Thompson Institute


Enzymatic reaction of: presilphiperfolan-8β-ol synthase

EC Number: 4.2.3.74

(2E,6E)-farnesyl diphosphate + H2O <=> presilphiperfolan-8β-ol + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: botrydial biosynthesis

Kinetic Parameters:

Substrate
Km (μM)
Citations
(2E,6E)-farnesyl diphosphate
6.04
[Pinedo08]


References

Pinedo08: Pinedo C, Wang CM, Pradier JM, Dalmais B, Choquer M, Le Pecheur P, Morgant G, Collado IG, Cane DE, Viaud M (2008). "Sesquiterpene synthase from the botrydial biosynthetic gene cluster of the phytopathogen Botrytis cinerea." ACS Chem Biol 3(12);791-801. PMID: 19035644


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, BIOCYC14B.