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MetaCyc Enzyme: α pyrone polyketide synthase

Gene: PKS Accession Number: G-11468 (MetaCyc)

Species: Plumbago indica

Summary:
A cDNA clone was isolated from the roots of plumbagin biosynthesis. The cDNA encoded a polyketide synthase, which was recombinantly expressed in E.coli and functionally characterized. The translated protein could catalyze the condensation of acetyl-CoA with 5 malonyl-CoA and produce an alpha pyrone. This novel PKS is involved in the biosynthesis of naphthoquinones [Springob07].

Molecular Weight of Polypeptide: 43.5 kD (from nucleotide sequence), 43.5 kD (experimental) [Springob07 ]

Unification Links: Entrez-Nucleotide:AB259100 , Entrez:BAF44539 , Protein Model Portal:A1IHK4 , SMR:A1IHK4 , UniProt:A1IHK4

Relationship Links: InterPro:IN-FAMILY:IPR001099 , InterPro:IN-FAMILY:IPR011141 , InterPro:IN-FAMILY:IPR012328 , InterPro:IN-FAMILY:IPR016038 , InterPro:IN-FAMILY:IPR016039 , InterPro:IN-FAMILY:IPR018088 , Pfam:IN-FAMILY:PF00195 , Pfam:IN-FAMILY:PF02797 , Prosite:IN-FAMILY:PS00441

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Credits:
Created 11-Aug-2009 by Pujar A , Boyce Thompson Institute


Enzymatic reaction of: hexaketide pyrone synthase (α pyrone polyketide synthase)

EC Number: 2.3.1.-

acetyl-CoA + 5 malonyl-CoA + 2 NADPH + 6 H+ + oxygen <=> hexaketide pyrone + 5 CO2 + 6 coenzyme A + 2 NADP+ + 3 H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.

In Pathways: plumbagin biosynthesis


Enzymatic reaction of: naphthylisoquinoline alkaloid precursor synthase (α pyrone polyketide synthase)

EC Number: 2.3.1.-

acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 8 H+ + oxygen <=> naphthylisoquinoline alkaloid precursor + 6 CO2 + 6 coenzyme A + 3 NADP+ + 4 H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: plumbagin biosynthesis


References

Springob07: Springob K, Samappito S, Jindaprasert A, Schmidt J, Page JE, De-Eknamkul W, Kutchan TM (2007). "A polyketide synthase of Plumbago indica that catalyzes the formation of hexaketide pyrones." FEBS J 274(2);406-17. PMID: 17229146


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Mon May 25, 2015, biocyc14.