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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: α pyrone polyketide synthase

Gene: PKS Accession Number: G-11468 (MetaCyc)

Species: Plumbago indica

Summary:
A cDNA clone was isolated from the roots of plumbagin biosynthesis. The cDNA encoded a polyketide synthase, which was recombinantly expressed in E.coli and functionally characterized. The translated protein could catalyze the condensation of acetyl-CoA with 5 malonyl-CoA and produce an alpha pyrone. This novel PKS is involved in the biosynthesis of naphthoquinones [Springob07].

Molecular Weight of Polypeptide: 43.5 kD (from nucleotide sequence), 43.5 kD (experimental) [Springob07 ]

Unification Links: Entrez-Nucleotide:AB259100 , Entrez:BAF44539 , Protein Model Portal:A1IHK4 , SMR:A1IHK4 , UniProt:A1IHK4

Relationship Links: InterPro:IN-FAMILY:IPR001099 , InterPro:IN-FAMILY:IPR011141 , InterPro:IN-FAMILY:IPR012328 , InterPro:IN-FAMILY:IPR016038 , InterPro:IN-FAMILY:IPR016039 , InterPro:IN-FAMILY:IPR018088 , Pfam:IN-FAMILY:PF00195 , Pfam:IN-FAMILY:PF02797 , Prosite:IN-FAMILY:PS00441

Gene-Reaction Schematic: ?

Credits:
Created 11-Aug-2009 by Pujar A , Boyce Thompson Institute


Enzymatic reaction of: hexaketide pyrone synthase (α pyrone polyketide synthase)

EC Number: 2.3.1.-

acetyl-CoA + 5 malonyl-CoA + 2 NADPH + 6 H+ + oxygen <=> hexaketide pyrone + 5 CO2 + 6 coenzyme A + 2 NADP+ + 3 H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.

In Pathways: plumbagin biosynthesis


Enzymatic reaction of: naphthylisoquinoline alkaloid precursor synthase (α pyrone polyketide synthase)

EC Number: 2.3.1.-

acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 8 H+ + oxygen <=> naphthylisoquinoline alkaloid precursor + 6 CO2 + 6 coenzyme A + 3 NADP+ + 4 H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: plumbagin biosynthesis


References

Springob07: Springob K, Samappito S, Jindaprasert A, Schmidt J, Page JE, De-Eknamkul W, Kutchan TM (2007). "A polyketide synthase of Plumbago indica that catalyzes the formation of hexaketide pyrones." FEBS J 274(2);406-17. PMID: 17229146


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc14.