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MetaCyc Enzyme: ginsenoside β- glucosidase

Species: Panax ginseng

Summary:
An enzyme was isolated from roots of Panax ginseng. The protein was purified using ion exchange chromatography and biochemically characterized. The enzyme catalyzed the conversion of (20S)-ginsenoside Rg3 to (20S)-ginsenoside Rh2, it hydrolyzes the β- (1- 2)-glucoside bond of (20S)-ginsenoside Rg3. It also exhibited considerable functional variation from other beta glucosidases [Zhang01c].

Molecular Weight of Polypeptide: 59.0 kD (experimental) [Zhang01c ]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Credits:
Created 07-Jan-2010 by Pujar A , Boyce Thompson Institute


Enzymatic reaction of: ginsenoside β- glucosidase

(20S)-ginsenoside Rg3 + H2O <=> (20S)-ginsenoside Rh2 + D-glucopyranose

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: ginsenoside degradation III

T(opt): 60 °C [Zhang01c]

pH(opt): 5 [Zhang01c]


References

Zhang01c: Zhang C, Yu H, Bao Y, An L, Jin F (2001). "Purification and characterization of ginsenoside-beta-glucosidase from ginseng." Chem Pharm Bull (Tokyo) 49(7);795-8. PMID: 11456082


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Thu Aug 27, 2015, biocyc14.