|Gene:||pqqE||Accession Number: G-11753 (MetaCyc)|
Species: Klebsiella pneumoniae
PqqE is a member of the radical SAM proteins, and is thus proposed to catalyze the C-C bond formation between the L-glutamate and L-tyrosine residues of the pyrroloquinoline quinone biosynthesis protein A. According to the model, the enzyme, using its 4Fe-4S center, produces a radical SAM molecule, which abstracts a hydrogen from the L-tyrosine residue, creating a tyrosine radical which reacts with the C9 carbon of the L-glutamate, leading to cyclization [Puehringer08].
Gene Citations: [Meulenberg92]
Molecular Weight of Polypeptide: 42.914 kD (from nucleotide sequence), 40.0 kD (experimental) [Magnusson04 ]
pI: 5.7 [Puehringer08]
Relationship Links: Entrez-Nucleotide:PART-OF:X58778 , InterPro:IN-FAMILY:IPR000385 , InterPro:IN-FAMILY:IPR006638 , InterPro:IN-FAMILY:IPR007197 , InterPro:IN-FAMILY:IPR011843 , InterPro:IN-FAMILY:IPR013785 , InterPro:IN-FAMILY:IPR017200 , InterPro:IN-FAMILY:IPR023885 , Pfam:IN-FAMILY:PF04055 , Pfam:IN-FAMILY:PF13186 , Prosite:IN-FAMILY:PS01305 , Smart:IN-FAMILY:SM00729
Enzymatic reaction of: glutamate-tyrosine ligase
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is favored in the direction shown.
In Pathways: pyrroloquinoline quinone biosynthesis
Magnusson04: Magnusson OT, Toyama H, Saeki M, Rojas A, Reed JC, Liddington RC, Klinman JP, Schwarzenbacher R (2004). "Quinone biogenesis: Structure and mechanism of PqqC, the final catalyst in the production of pyrroloquinoline quinone." Proc Natl Acad Sci U S A 101(21);7913-8. PMID: 15148379
Velterop95: Velterop JS, Sellink E, Meulenberg JJ, David S, Bulder I, Postma PW (1995). "Synthesis of pyrroloquinoline quinone in vivo and in vitro and detection of an intermediate in the biosynthetic pathway." J Bacteriol 177(17);5088-98. PMID: 7665488
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