Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: D-glucarate dehydratase

Gene: gudD Accession Number: G-11994 (MetaCyc)

Species: Acinetobacter sp. ADP1

Subunit composition of D-glucarate dehydratase = [GudD]4
         D-glucarate dehydratase subunit = GudD

Summary:
The enzyme from Acinetobacter sp. ADP1 (previously known as Acinetobacter baylyi ADP1) was reported to be a homotetramer based on gel filtration data, but the native apparent molecular mass was not given [Aghaie08].

Recombinant enzyme was overexpressed in Escherichia coli and purified [Aghaie08].

The crystal structure of D-glucarate dehydratase from Pseudomonas putida has been determined [Gulick98].
Note that [Aghaie08] provided a subunit apparent molecular mass of 38 kDa as determined by SDS-PAGE, along with a calculated molecular mass of 34.96 kDa. However, these numbers do not agree with the computed molecular mass of 49.027 kDa found for this entry in the UniProt link below, dated 2004.

Map Position: [132,209 -> 133,543]

Molecular Weight of Polypeptide: 34.96 kD (from nucleotide sequence), 38.0 kD (experimental) [Aghaie08 ]

Unification Links: Entrez-gene:2879250 , Protein Model Portal:Q6FFQ2 , SMR:Q6FFQ2 , String:62977.ACIAD0128 , UniProt:Q6FFQ2

Relationship Links: InterPro:IN-FAMILY:IPR001354 , InterPro:IN-FAMILY:IPR013342 , InterPro:IN-FAMILY:IPR017653 , Panther:IN-FAMILY:PTHR13794 , Pfam:IN-FAMILY:PF01188 , Smart:IN-FAMILY:SM00922

Gene-Reaction Schematic: ?

Credits:
Created 26-Apr-2010 by Fulcher CA , SRI International


Enzymatic reaction of: D-glucarate dehydratase

EC Number: 4.2.1.40

D-glucarate <=> 5-dehydro-4-deoxy-D-glucarate + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of microbial D-galacturonate and D-glucuronate degradation , D-glucarate degradation II

Kinetic Parameters:

Substrate
Km (μM)
Citations
D-glucarate
101.0
[Aghaie08]


References

Aghaie08: Aghaie A, Lechaplais C, Sirven P, Tricot S, Besnard-Gonnet M, Muselet D, de Berardinis V, Kreimeyer A, Gyapay G, Salanoubat M, Perret A (2008). "New insights into the alternative D-glucarate degradation pathway." J Biol Chem 283(23);15638-46. PMID: 18364348

Gulick98: Gulick AM, Palmer DR, Babbitt PC, Gerlt JA, Rayment I (1998). "Evolution of enzymatic activities in the enolase superfamily: crystal structure of (D)-glucarate dehydratase from Pseudomonas putida." Biochemistry 37(41);14358-68. PMID: 9772161


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC13A.