|Gene:||ACIAD0131||Accession Number: G-11996 (MetaCyc)|
Species: Acinetobacter sp. ADP1
The enzyme from Acinetobacter sp. ADP1 (Acinetobacter baylyi ADP1) was reported to be a homodimer based on gel filtration data, but the native apparent molecular mass was not given [Aghaie08].
Recombinant enzyme was overexpressed in Escherichia coli and purified [Aghaie08].
Note that [Aghaie08] provided a subunit apparent molecular mass of 62 kDa as determined by SDS-PAGE, along with a calculated molecular mass of 58.48 kDa. A computed molecular mass of 56.145 kDa was found for this entry in the UniProt link below dated 2004.
The gene is designated here by its locus tag.
|Map Position: [135,198 -> 136,778]|
Molecular Weight of Polypeptide: 58.48 kD (from nucleotide sequence), 62.0 kD (experimental) [Aghaie08 ]
Relationship Links: InterPro:IN-FAMILY:IPR015590 , InterPro:IN-FAMILY:IPR016161 , InterPro:IN-FAMILY:IPR016162 , InterPro:IN-FAMILY:IPR016163 , Pfam:IN-FAMILY:PF00171 , Prosite:IN-FAMILY:PS00070 , Prosite:IN-FAMILY:PS00687
Enzymatic reaction of: α-ketoglutarate semialdehyde dehydrogenase
Synonyms: 2-ketoglutarate semialdehyde dehydrogenase
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
Depending upon the assay, the kinetics of the reaction were either sigmoidal, or hyperbolic. In contrast, a Pseudomonas putida α-ketoglutarate semialdehyde dehydrogenase isozyme encoded by locus tag PP3602 showed only hyperbolic kinetics. Although the enzyme was active with both NAD+ and NADP+, it was 58 times more active with NADP+. The Km value shown here was determined from a hyperbolic Vmax model with NADP+. The enzyme was relatively substrate-specific [Aghaie08].
Aghaie08: Aghaie A, Lechaplais C, Sirven P, Tricot S, Besnard-Gonnet M, Muselet D, de Berardinis V, Kreimeyer A, Gyapay G, Salanoubat M, Perret A (2008). "New insights into the alternative D-glucarate degradation pathway." J Biol Chem 283(23);15638-46. PMID: 18364348
©2014 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493