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MetaCyc Enzyme: sulfolactate dehydrogenase

Gene: slcD Accession Numbers: G-12225 (MetaCyc), ISM_13310

Species: Roseovarius nubinhibens ISM

Summary:
SlcD catalyzes a sulfolactate dehydrogenase activity in cell extracts of Roseovarius nubinhibens ISM. Activity was dependent on an artificail electron acceptor (ferricyanide), and could not be restored with NADP+, NAD+, FAD, FMN or cytochrome c [Denger09].

The enzyme was found in both the soluble and particulate fractions of the cell extract, but activity in the particulate fraction was much higher, indicating that the enzyme is membrane-bound. Activity with (S)-lactate was about 10% of the activity with (RS)-3-sulfolactate.

The enzyme was partially (10-fold) purified, but purity was not sufficient to obtain amino acid sequence. However, the enzyme is believed to be encoded by the slcD gene, since it is present in the same operon as other enzymes involved in sulfolactate degradation, and encodes a membrane-bound 2-hydroxyacid dehydrogenase. In addition, the gene is transcribed inducibly during growth on sulfolactate [Denger09].

Molecular Weight of Polypeptide: 41.384 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:A3SN06 , UniProt:A3SN06

Relationship Links: InterPro:IN-FAMILY:IPR000262 , InterPro:IN-FAMILY:IPR012133 , InterPro:IN-FAMILY:IPR013785 , Pfam:IN-FAMILY:PF01070 , Prosite:IN-FAMILY:PS51349

Gene-Reaction Schematic: ?

Credits:
Created 05-Oct-2010 by Caspi R , SRI International


Enzymatic reaction of: sulfolactate dehydrogenase

EC Number: 1.1.1.-

(RS)-3-sulfolactate + an oxidized electron acceptor <=> 3-sulfopyruvate + a reduced electron acceptor

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

Alternative Substrates for (RS)-3-sulfolactate: (S)-lactate [Denger09 ]

In Pathways: superpathway of sulfolactate degradation , sulfolactate degradation II , sulfolactate degradation III

Cofactors or Prosthetic Groups: FMN [Denger09]


References

Denger09: Denger K, Mayer J, Buhmann M, Weinitschke S, Smits TH, Cook AM (2009). "Bifurcated degradative pathway of 3-sulfolactate in Roseovarius nubinhibens ISM via sulfoacetaldehyde acetyltransferase and (S)-cysteate sulfolyase." J Bacteriol 191(18);5648-56. PMID: 19581363


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC14B.