Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: salicylyl-CoA synthase

Gene: sdgB Accession Number: G-12234 (MetaCyc)

Species: Streptomyces sp. WA46

Summary:
The subunit structure of this enzyme has not been reported.

Streptomyces sp. WA46 was isolated from soil and shown to degrade salicylate via a novel pathway involving its coenzyme A derivative (see pathway salicylate degradation IV). A plasmid containing a gene cluster sdgABCDEFGHR from Streptomyces sp. WA46 was constructed and shown to be sufficient for the complete degradation of salicylate. Derivatives disrupted in each gene were created and transformed into the heterologous host Streptomyces lividans TK64. salicylate degradation was blocked by disruption of genes sdgA and sdgC and gentisate accumulation resulted from disruption of sdgD [Ishiyama04].

Expression of individual genes in Escherichia coli and assay of enzyme activities in cell extracts suggested their function. Gene sdgA was required for the conversion of salicylate to salicyloyl-CoA with cofactors ATP and coenzyme A via a postulated salicylate-adenylate intermediate. The product of gene sdgB had amino acid sequence similarity to acetyl-CoA synthetases. It was hypothesized to be involved in coenzyme A addition [Ishiyama04].

Molecular Weight of Polypeptide: 70.197 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:Q7X280 , UniProt:Q7X280

Relationship Links: Entrez-Nucleotide:PART-OF:AB112586 , InterPro:IN-FAMILY:IPR003781 , InterPro:IN-FAMILY:IPR011761 , InterPro:IN-FAMILY:IPR016040 , InterPro:IN-FAMILY:IPR016102 , Pfam:IN-FAMILY:PF13380 , Prosite:IN-FAMILY:PS50975 , Smart:IN-FAMILY:SM00881

Gene-Reaction Schematic: ?

Credits:
Created 06-Oct-2010 by Fulcher CA , SRI International


Enzymatic reaction of: salicylyl-CoA synthase

Synonyms: salicyl-CoA synthetase

salicylate-adenylate + coenzyme A <=> salicyloyl-CoA + AMP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: salicylate degradation IV

Summary:
This reaction shows the conversion of the postulated salicylate-adenylate intermediate to salicyloyl-CoA. The product of gene sdgB was proposed to be involved in coenzyme A addition in this reaction although this was not demonstrated. salicyloyl-CoA was produced by SdgA in the presence or absence of SdgB [Ishiyama04].


References

Ishiyama04: Ishiyama D, Vujaklija D, Davies J (2004). "Novel pathway of salicylate degradation by Streptomyces sp. strain WA46." Appl Environ Microbiol 70(3);1297-306. PMID: 15006746


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, BIOCYC14A.