Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
twitter

MetaCyc Enzyme: GDP-glucosamine N-acetyltransferase

Gene: glmU Accession Number: G-12615 (MetaCyc)

Synonyms: Cj0821

Species: Campylobacter jejuni jejuni NCTC 11168 = ATCC 700819

Summary:
The subunit structure of this enzyme has not been reported.

In the food-borne pathogen Campylobacter jejuni this enzyme was proposed to participate in the biosynthesis of CMP-N,N'-diacetyllegionaminate (CMP-legionaminic acid), a virulence-associated, cell surface sialic acid-like derivative (see pathway CMP-legionaminate biosynthesis I). Although GlmU was proposed to catalyze the acetylation of GDP-D-glucosamine, the reaction did not go to completion as compared with its known substrate D-glucosamine 1-phosphate, using capillary electrophoresis/mass spectrometry based assays. Other putative N-acetyltransferases from the Campylobacter jejuni jejuni NCTC 11168 = ATCC 700819 flagellar glycosylation locus that can efficiently catalyze this reaction are also being screened. In Escherichia coli GlmU is a bifunctional enzyme responsible for both the acetylation of D-glucosamine 1-phosphate and uridylation of N-acetyl-α-D-glucosamine 1-phosphate (see fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase) [Schoenhofen09].

Recombinant, N-terminal His6-tagged polypeptide was purified and characterized [Schoenhofen09].

Map Position: [768,906 -> 770,195]

Molecular Weight of Polypeptide: 47.906 kD (from nucleotide sequence)

Unification Links: Entrez-gene:905125 , Protein Model Portal:Q9PPA2 , String:192222.Cj0821 , UniProt:Q9PPA2

Relationship Links: InterPro:IN-FAMILY:IPR001451 , InterPro:IN-FAMILY:IPR005882 , InterPro:IN-FAMILY:IPR011004 , InterPro:IN-FAMILY:IPR025877 , Panther:IN-FAMILY:PTHR22572:SF17 , Pfam:IN-FAMILY:PF00132 , Pfam:IN-FAMILY:PF12804 , Prosite:IN-FAMILY:PS00101

Gene-Reaction Schematic: ?

Credits:
Created 16-Mar-2011 by Fulcher CA , SRI International


Enzymatic reaction of: GDP-glucosamine N-acetyltransferase

EC Number: 2.3.1.-

GDP-D-glucosamine + acetyl-CoA <=> GDP-N-acetyl-D-glucosamine + coenzyme A + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

Alternative Substrates for GDP-D-glucosamine: D-glucosamine 1-phosphate [Schoenhofen09 ]

In Pathways: CMP-legionaminate biosynthesis I


References

Schoenhofen09: Schoenhofen IC, Vinogradov E, Whitfield DM, Brisson JR, Logan SM (2009). "The CMP-legionaminic acid pathway in Campylobacter: biosynthesis involving novel GDP-linked precursors." Glycobiology 19(7);715-25. PMID: 19282391


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Feb 27, 2015, BIOCYC14A.