MetaCyc Enzyme: CMP-legionaminic acid synthase

Gene: legF Accession Number: G-12623 (MetaCyc)

Synonyms: Cj1331, NeuA3, PtmB

Species: Campylobacter jejuni jejuni NCTC 11168 = ATCC 700819

The subunit structure of this enzyme has not been reported.

Recombinant, C-terminal His6-tagged polypeptide was purified and characterized [Schoenhofen09].

In the food-borne pathogen Campylobacter jejuni this enzyme participates in the biosynthesis of CMP-N,N'-diacetyllegionaminate (CMP-legionaminic acid), a virulence-associated, cell surface sialic acid-like derivative (see pathway CMP-legionaminate biosynthesis I). Leg F catalyzes the synthesis of CMP-N,N'-diacetyllegionaminate (CMP-activated legionaminic acid) and is a homolog of Escherichia coli NeuA (see cytidine 5'-monophosphate N-acetylneuraminate synthetase) [Schoenhofen09].

Map Position: [1,258,212 -> 1,258,919]

Molecular Weight of Polypeptide: 26.661 kD (from nucleotide sequence)

Unification Links: Entrez-gene:905623 , Protein Model Portal:Q0P8S7 , String:192222.Cj1331 , UniProt:Q0P8S7

Relationship Links: InterPro:IN-FAMILY:IPR003329 , InterPro:IN-FAMILY:IPR029044 , Pfam:IN-FAMILY:PF02348

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 16-Mar-2011 by Fulcher CA , SRI International

Enzymatic reaction of: CMP-legionaminate synthase (CMP-legionaminic acid synthase)

EC Number:

CTP + N, N'-diacetyllegionaminate <=> CMP-N,N'-diacetyllegionaminate + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: CMP-legionaminate biosynthesis I

This enzyme could efficiently catalyze production of CMP-N,N'-diacetyllegionaminate (CMP-legionaminic acid). Metabolites were purified and identified by capillary electrophoresis/mass spectrometry analysis and NMR spectroscopy [Schoenhofen09].


Schoenhofen09: Schoenhofen IC, Vinogradov E, Whitfield DM, Brisson JR, Logan SM (2009). "The CMP-legionaminic acid pathway in Campylobacter: biosynthesis involving novel GDP-linked precursors." Glycobiology 19(7);715-25. PMID: 19282391

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Thu Oct 8, 2015, biocyc13.