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discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: 2-keto-3-deoxyxylonate dehydratase

Gene: HVO_B0027 Accession Number: G-12646 (MetaCyc)

Species: Haloferax volcanii

Summary:
The subunit structure of this enzyme has not been reported.

This dehydratase was shown to be essential for α-D-xylopyranose (D-xylose) degradation in the halophilic archaeon Haloferax volcanii. Orthologs with 63% and 56% amino acid sequence identity were found in Haloarcula marismortui and Halorubrum lacusprofundi, respectively. A characterized ortholog in the archaeon Sulfolobus solfataricus was 40% identical and a putative ortholog in the bacterium Caulobacter crescentus CB15 was 18% identical. These enzymes belong to the fumaroylacetoacetate hydrolase superfamily [Johnsen09].

An in-frame deletion mutant of the gene encoding this enzyme resulted in inability to grow on α-D-xylopyranose, while growth on D-glucose was unaffected [Johnsen09].

DNA microarray analyses showed that the transcription of this gene was highly up-regulated by growth on α-D-xylopyranose. It formed part of a transcription unit with HVO_B0028 encoding D-xylose dehydrogenase [Johnsen09].

The genome of this organism is composed of a main chromosome of 2.848 Mb, three smaller chromosomes (pHV4, pHV3 and pHV1) and a plasmid pHV2 [Hartman10]. This gene is located on pHV3.

Map Position: [29,918 -> 30,787]

Molecular Weight of Polypeptide: 31.862 kD (from nucleotide sequence)

Unification Links: Entrez-gene:8919100 , Protein Model Portal:D4GP28 , UniProt:D4GP28

Relationship Links: InterPro:IN-FAMILY:IPR002529 , InterPro:IN-FAMILY:IPR011234 , Pfam:IN-FAMILY:PF01557

Gene-Reaction Schematic: ?

Credits:
Created 18-Mar-2011 by Fulcher CA , SRI International


Enzymatic reaction of: 2-keto-3-deoxyxylonate dehydratase

EC Number: 4.2.1.141

2-dehydro-3-deoxy-D-arabinonate <=> 2,5-dioxopentanoate + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of pentose and pentitol degradation , xylose degradation III


References

Hartman10: Hartman AL, Norais C, Badger JH, Delmas S, Haldenby S, Madupu R, Robinson J, Khouri H, Ren Q, Lowe TM, Maupin-Furlow J, Pohlschroder M, Daniels C, Pfeiffer F, Allers T, Eisen JA (2010). "The complete genome sequence of Haloferax volcanii DS2, a model archaeon." PLoS One 5(3);e9605. PMID: 20333302

Johnsen09: Johnsen U, Dambeck M, Zaiss H, Fuhrer T, Soppa J, Sauer U, Schonheit P (2009). "D-xylose degradation pathway in the halophilic archaeon Haloferax volcanii." J Biol Chem 284(40);27290-303. PMID: 19584053


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc13.