|Gene:||AT9||Accession Number: G-12810 (MetaCyc)|
Species: Actinidia eriantha
A cDNA clone encoding an acetyl-CoA:alcohol O-acyltransferase was isolated from A. deliciosa tissue and recombinantly expressed in yeast. The gene was named AT9.
The recombinant protein was characterized for its substrate specificity, and exhibited alcohol acyl transferase activity (AAT) with a broad range of alcohol and acyl-CoA substrates leading to the formation of volatile esters commonly found in kiwifruit [Gunther11].
Transcript accumulation increased by several folds in ripe versus unripe fruit.
Molecular Weight of Polypeptide: 47.67 kD (from nucleotide sequence)
Unification Links: Entrez-Nucleotide:HO772637
Enzymatic reaction of: acetyl-CoA:butanol O-acyltransferase (acetyl-CoA:alcohol O-acyltransferase)
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: volatile esters biosynthesis (during fruit ripening)
Gunther11: Gunther CS, Chervin C, Marsh KB, Newcomb RD, Souleyre EJ (2011). "Characterisation of two alcohol acyltransferases from kiwifruit (Actinidia spp.) reveals distinct substrate preferences." Phytochemistry 72(8);700-10. PMID: 21450321
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