MetaCyc Enzyme: serine hydroxymethyltransferase

Gene: glyA Accession Number: G-3267 (MetaCyc)

Synonyms: SHMT

Species: Methylobacterium extorquens AM1

The serine hydroxymethyltransferase from M. extorquens has not been purified, and little is known about it. The gene glyA was cloned, and mutants which were disruppted in this gene were created. The mutants failed to grow on methanol, methylamine, ethanol, and ethylamine. Introduction of a plasmid with the cloned gene into the mutants restored wild type phenotype. The subunit structure of the enzyme or the size of its components were not studied.

Locations: cytosol

Molecular Weight of Polypeptide: 46.3 kD (from nucleotide sequence)

pI: 6.0 [Chistoserdova94]

Relationship Links: Entrez-Nucleotide:PART-OF:L33463

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Cellular Component: GO:0005829 - cytosol

MultiFun Terms: metabolism carbon utilization carbon compounds

Enzymatic reaction of: serine hydroxymethyltransferase

EC Number:

L-serine + a tetrahydrofolate <=> glycine + a 5,10-methylene-tetrahydrofolate + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

This reaction is reversible.

In Pathways: formaldehyde assimilation I (serine pathway) , folate transformations I

Serine hydroxymethyltransferase catalyzes the reversible conversion of serine to glycine and tetrahydrofolate to 5,10-methylene tetrahydrofolate.

Citations: [Trivedi02]


Chistoserdova94: Chistoserdova LV, Lidstrom ME (1994). "Genetics of the serine cycle in Methylobacterium extorquens AM1: cloning, sequence, mutation, and physiological effect of glyA, the gene for serine hydroxymethyltransferase." J Bacteriol 176(21);6759-62. PMID: 7961431

Trivedi02: Trivedi V, Gupta A, Jala VR, Saravanan P, Rao GS, Rao NA, Savithri HS, Subramanya HS (2002). "Crystal structure of binary and ternary complexes of serine hydroxymethyltransferase from Bacillus stearothermophilus: insights into the catalytic mechanism." J Biol Chem 277(19);17161-9. PMID: 11877399

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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